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• W2164927499 abstract "ClC chloride channels, which are ubiquitously expressed in mammals, have a unique double-barreled structure, in which each monomer forms its own pore. Identification of pore-lining elements is important for understanding the conduction properties and unusual gating mechanisms of these channels. Structures of prokaryotic ClC transporters do not show an open pore, and so may not accurately represent the open state of the eukaryotic ClC channels. In this study we used cysteine-scanning mutagenesis and modification (SCAM) to screen &gt;50 residues in the intracellular vestibule of ClC-0. We identified 14 positions sensitive to the negatively charged thiol-modifying reagents sodium (2-sulfonatoethyl)methanethiosulfonate (MTSES) or sodium 4-acetamido-4'-maleimidylstilbene-2'2-disulfonic acid (AMS) and show that 11 of these alter pore properties when modified. In addition, two MTSES-sensitive residues, on different helices and in close proximity in the prokaryotic structures, can form a disulfide bond in ClC-0. When mapped onto prokaryotic structures, MTSES/AMS-sensitive residues cluster around bound chloride ions, and the correlation is even stronger in the ClC-0 homology model developed by Corry et al. (2004). These results support the hypothesis that both secondary and tertiary structures in the intracellular vestibule are conserved among ClC family members, even in regions of very low sequence similarity." @default.
• W2164927499 created "2016-06-24" @default.
• W2164927499 creator A5081962062 @default.
• W2164927499 creator A5084403209 @default.
• W2164927499 date "2005-05-16" @default.
• W2164927499 modified "2023-10-10" @default.
• W2164927499 title "Cysteine Accessibility in ClC-0 Supports Conservation of the ClC Intracellular Vestibule" @default.
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• W2164927499 cites W1592442520 @default.
• W2164927499 cites W1600283399 @default.
• W2164927499 cites W1969540269 @default.
• W2164927499 cites W1969628201 @default.
• W2164927499 cites W1970251430 @default.
• W2164927499 cites W1976444135 @default.
• W2164927499 cites W1977082660 @default.
• W2164927499 cites W1978453821 @default.
• W2164927499 cites W1979001025 @default.
• W2164927499 cites W1979694517 @default.
• W2164927499 cites W1979963779 @default.
• W2164927499 cites W1986736656 @default.
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• W2164927499 cites W1994088425 @default.
• W2164927499 cites W1994522080 @default.
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• W2164927499 cites W1998401151 @default.
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• W2164927499 cites W2005246577 @default.
• W2164927499 cites W2005501525 @default.
• W2164927499 cites W2005635768 @default.
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• W2164927499 cites W2028127482 @default.
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• W2164927499 cites W2040446606 @default.
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• W2164927499 doi "https://doi.org/10.1085/jgp.200509258" @default.
• W2164927499 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2234078" @default.
• W2164927499 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15897295" @default.
• W2164927499 hasPublicationYear "2005" @default.
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