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• W2165032016 abstract "Our objective was to determine whether the concentration of cooled sperm inseminated influenced sperm transport and intensity of the uterine inflammatory reaction 2, 4 and 24 h after insemination. Experimental subjects were 189 estrous mares with a dominant follicle ≥35 mm in diameter and no bacterial growth or neutrophils detected in uterine smears. Each mare was randomly assigned to receive one of the following intrauterine treatments (volume, 20 mL): insemination with 5 × 106 mL−1 or 25 × 106 mL−1 or 50 × 106 mL−1 sperm diluted in 3 mL seminal plasma (SP) and 17 mL skim milk; seminal plasma or skim milk extender. Mares in a control group received no intrauterine treatment. Mares were slaughtered 2, 4 or 24 h after insemination or infusion. Oviducts were separated from the uterus, and uterus and oviducts were then flushed with phosphate-buffered saline (PBS). After flushing, an endometrial sample was collected for further histopathological examination. The grade of uterine fibrosis and the amount of neutrophils in the stratum compactum were evaluated. A sample of each tubal flushing was examined for sperm count, and a sample of each uterine flushing was examined for PMN count. It was concluded that compounds in the insemination dose provoked a uterine inflammatory response, which was more rapid and intense as sperm concentration increased. In contrast, sperm transport through 4 h after insemination was not influenced by sperm concentration." @default.
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• W2165032016 date "2007-02-01" @default.
• W2165032016 modified "2023-09-30" @default.
• W2165032016 title "Effect of sperm numbers and concentration on sperm transport and uterine inflammatory response in the mare" @default.
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• W2165032016 doi "https://doi.org/10.1016/j.theriogenology.2006.09.005" @default.
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