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• W2165200137 abstract "Objective. To study the effects of progesterone (P) and 18‐methyl levonorgestrel (LNG) on the expression of P receptors (PRA and PRB), c‐fos, c‐jun, and osteocalcin in the human osteosarcoma cell line MG‐63 and human normal osteoblasts in order to understand the mechanism of progestin action on the proliferation and differentiation of osteoblasts. Methods. Cell proliferation was tested by MTT assay. The expression of PR, c‐fos, c‐jun, and osteocalcin was measured by semi‐quantitative RT‐PCR, Western immunoblot or immunocytochemistry. Results. Progesterone and LNG (10−10–10−6 M) stimulated cell proliferation in a dose‐dependent manner. Progesterone and LNG did not affect the expression of PRA and PRB mRNA and protein, but c‐fos and c‐jun mRNA and protein were upregulated in a dose‐dependent manner. The expression of osteocalcin mRNA was also increased in human osteoblasts in a dose and time‐dependent manner with greater effects of LNG than P, while the expression of osteocalcin mRNA in MG‐63 cells was not changed by P or LNG. Conclusion. Progesterone and LNG promote osteocalcin gene transcription by stimulating the expression of c‐fos and c‐jun, and result in osteoblast proliferation and differentiation." @default.
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• W2165200137 date "2003-01-01" @default.
• W2165200137 modified "2023-10-13" @default.
• W2165200137 title "Effects of Progesterone and 18‐Methyl Levonorgestrel on Osteoblastic Cells" @default.
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• W2165200137 doi "https://doi.org/10.1081/erc-120026954" @default.
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