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• W2165277846 abstract "The X-ray crystal structure of an arginase-like protein from the parasitic protozoan Trypanosoma brucei, designated TbARG, is reported at 1.80 and 2.38 Å resolution in its reduced and oxidized forms, respectively. The oxidized form of TbARG is a disulfide-linked hexamer that retains the overall architecture of a dimer of trimers in the reduced form. Intriguingly, TbARG does not contain metal ions in its putative active site, and amino acid sequence comparisons indicate that all but one of the residues required for coordination to the catalytically obligatory binuclear manganese cluster in other arginases are substituted here with residues incapable of metal ion coordination. Therefore, the structure of TbARG is the first of a member of the arginase/deacetylase superfamily that is not a metalloprotein. Although we show that metal binding activity is easily reconstituted in TbARG by site-directed mutagenesis and confirmed in X-ray crystal structures, it is curious that this protein and its parasitic orthologues evolved away from metal binding function. Knockout of the TbARG gene from the genome demonstrated that its function is not essential to cultured bloodstream-form T. brucei, and metabolomics analysis confirmed that the enzyme has no role in the conversion of l-arginine to l-ornithine in these cells. While the molecular function of TbARG remains enigmatic, the fact that the T. brucei genome encodes only this protein and not a functional arginase indicates that the parasite must import l-ornithine from its host to provide a source of substrate for ornithine decarboxylase in the polyamine biosynthetic pathway, an active target for the development of antiparasitic drugs." @default.
• W2165277846 created "2016-06-24" @default.
• W2165277846 creator A5015579596 @default.
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• W2165277846 creator A5073175338 @default.
• W2165277846 date "2014-12-23" @default.
• W2165277846 modified "2023-09-27" @default.
• W2165277846 title "Crystal Structure of an Arginase-like Protein from <i>Trypanosoma brucei</i> That Evolved without a Binuclear Manganese Cluster" @default.
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• W2165277846 doi "https://doi.org/10.1021/bi501366a" @default.
• W2165277846 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4303290" @default.
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• W2165277846 hasPublicationYear "2014" @default.
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