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• W2165371657 abstract "Sequence differences in the noncoding region of the human papillomavirus type 16 (HPV-16) genome were displayed using single-stranded conformational polymorphism (SSCP) analysis of polymerase chain reaction (PCR)-amplified material. Two variants accounted for 50%–70% of all HPV-16 variants from 3 cohorts in Seattle. Seventy subjects who were repeatedly HPV-16 DNA-positive over 2–8 4-monthly visits showed an identical SSCP pattern at every visit. Only 10%–20% of the specimens showed evidence of infection by multiple variants when assessed by SSCP. However, cloning and sequencing of the PCR products revealed a substantially higher proportion of specimens with > 1 variant. Sequencing many clones from each specimen confirmed that 1 major variant seemed to predominate over time, whereas minor variants appeared more transient. These results suggest that HPV-16 establishes a persistent infection in which a single variant predominates: coinfection with additional HPV-16 variants results in a minor population of HPV-16 genomes." @default.
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• W2165371657 date "1995-09-01" @default.
• W2165371657 modified "2023-10-18" @default.
• W2165371657 title "Analysis of Human Papillomavirus Type 16 Variants Indicates Establishment of Persistent Infection" @default.
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• W2165371657 doi "https://doi.org/10.1093/infdis/172.3.747" @default.
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