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- W2165420669 abstract "Double fertilization of the egg cell and the central cell by two sperm cells, resulting in the formation of the embryo and the endosperm, respectively, is a defining characteristic of flowering plants. The Arabidopsis thaliana female gametophytic mutant glauce (glc) can exhibit embryo development without any endosperm. Here, we show that in glc mutant embryo sacs one sperm cell successfully fuses with the egg cell but the second sperm cell fails to fuse with the central cell, resulting in single fertilization. Complementation studies using genes from the glc deletion interval identified an unusual genomic locus having homology to BAHD (for BEAT, AHCT, HCBT, and DAT) acyl-transferases with dual transcription units and alternative splicing that could rescue the sterility defect of glc. Expression of these transcripts appears restricted to the central cell, and expression within the central cell is sufficient to restore fertility. We conclude that the central cell actively promotes its own fertilization by the sperm cell through a signaling mechanism involving products of At1g65450. Successful fertilization of the egg cell is not blocked in the glc mutant, suggesting that evolution of double fertilization in flowering plants involved acquisition of specific functions by the central cell to enable its role as a second female gamete." @default.
- W2165420669 created "2016-06-24" @default.
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- W2165420669 date "2012-08-01" @default.
- W2165420669 modified "2023-10-13" @default.
- W2165420669 title "Molecular Characterization of the <i>glauce</i> Mutant: A Central Cell–Specific Function Is Required for Double Fertilization in <i>Arabidopsis</i>" @default.
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- W2165420669 doi "https://doi.org/10.1105/tpc.112.096420" @default.
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