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• W2165465840 abstract "ABSTRACT We describe the development of genetic tools for regulated gene expression, the introduction of chromosomal mutations, and improved plasmid transfer by electroporation in the food-borne pathogen Listeria monocytogenes . pIMK, a kanamycin-resistant, site-specific, integrative listeriophage vector was constructed and then modified for overexpression (pIMK2) or for isopropyl-β- d -thiogalactopyranoside (IPTG)-regulated expression (pIMK3 and pIMK4). The dynamic range of promoters was assessed by determining luciferase activity, P60 secretion, and internalin A-mediated invasion. These analyses demonstrated that pIMK4 and pIMK3 have a stringently controlled dynamic range of 540-fold. Stable gene overexpression was achieved with pIMK2, giving a range of expression for the three vectors of 1,350-fold. The lactococcal pORI280 system was optimized for the generation of chromosomal mutations and used to create five new prfA star mutants. The combination of pIMK4 and pORI280 allowed streamlined creation of “IPTG-dependent” mutants. This was exemplified by creation of a clean deletion mutant with deletion of the universally essential secA gene, and this mutant exhibited a rapid loss of viability upon withdrawal of IPTG. We also improved plasmid transfer by electroporation into three commonly used laboratory strains of L. monocytogenes . A 125-fold increase in transformation efficiency for EGDe compared with the widely used protocol of Park and Stewart (S. F. Park and G. S. Stewart, Gene 94:129-132, 1990) was observed. Maximal transformation efficiencies of 5.7 × 10 6 and 6.7 × 10 6 CFU per μg were achieved for EGDe and 10403S, respectively, with a replicating plasmid. An efficiency of 2 × 10 7 CFU per μg is the highest efficiency reported thus far for L. monocytogenes F2365." @default.
• W2165465840 created "2016-06-24" @default.
• W2165465840 creator A5007896897 @default.
• W2165465840 creator A5017416740 @default.
• W2165465840 creator A5047441756 @default.
• W2165465840 date "2008-07-01" @default.
• W2165465840 modified "2023-10-04" @default.
• W2165465840 title "Tools for Functional Postgenomic Analysis of<i>Listeria monocytogenes</i>" @default.
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• W2165465840 cites W1552307351 @default.
• W2165465840 cites W1581133875 @default.
• W2165465840 cites W1591758727 @default.
• W2165465840 cites W1617668023 @default.
• W2165465840 cites W1821737407 @default.
• W2165465840 cites W1827851367 @default.
• W2165465840 cites W1876341706 @default.
• W2165465840 cites W1966327293 @default.
• W2165465840 cites W1968477104 @default.
• W2165465840 cites W1975165965 @default.
• W2165465840 cites W1978163337 @default.
• W2165465840 cites W1983584781 @default.
• W2165465840 cites W1987838634 @default.
• W2165465840 cites W1991634127 @default.
• W2165465840 cites W1993637980 @default.
• W2165465840 cites W1996827942 @default.
• W2165465840 cites W1997297277 @default.
• W2165465840 cites W2006495190 @default.
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• W2165465840 cites W2035752210 @default.
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• W2165465840 cites W2049431015 @default.
• W2165465840 cites W2054626788 @default.
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• W2165465840 doi "https://doi.org/10.1128/aem.00314-08" @default.
• W2165465840 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2446514" @default.
• W2165465840 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18441118" @default.
• W2165465840 hasPublicationYear "2008" @default.
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