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• W2165514587 abstract "Abstract Aim To evaluate whether and how the transcription factor early growth response gene 1 ( EGR 1) affects the osteogenic differentiation of dental stem cells. Methodology Dental stem cells from apical papilla ( SCAP s) and from the dental follicle ( DFC s) were transfected with EGR 1‐specific si RNA or EGR ‐1 expression plasmid. Gene regulation was verified at protein level by W estern blotting. The expression of the transcription factors distal‐less homeobox 3 ( DLX 3), alkaline phosphatase ( ALP ) and bone morphogenetic protein 2 ( BMP 2), which are all regulators and markers of the osteogenic differentiation in dental stem cells, was determined by quantitative reverse transcription‐polymerase chain reaction (q RT ‐ PCR ). To investigate mineralization, SCAP long‐term cultures were stained with alizarin red after EGR 1 over‐expression. Results EGR 1 was induced in SCAP s during osteogenic differentiation. DLX 3 and bone morphogenetic protein 2 ( BMP 2) were up‐regulated after EGR 1 over‐expression and down‐regulated after EGR 1 depletion. The expression of ALP was also down‐regulated after EGR 1 depletion. The over‐expression of EGR 1 in SCAP s promoted mineralization after osteogenic differentiation. Conclusions EGR 1 supported the osteogenic differentiation of dental stem cells by potentially regulating the expression of DLX 3 and BMP 2." @default.
• W2165514587 created "2016-06-24" @default.
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• W2165514587 date "2014-06-25" @default.
• W2165514587 modified "2023-10-11" @default.
• W2165514587 title "<scp>EGR</scp>1 supports the osteogenic differentiation of dental stem cells" @default.
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• W2165514587 doi "https://doi.org/10.1111/iej.12299" @default.
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