FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2165573190> ?p ?o ?g. }

• W2165573190 endingPage "9" @default.
• W2165573190 startingPage "6663" @default.
• W2165573190 abstract "Single-chain Fv antibody fragments from the CD20-specific murine monoclonal antibody B9E9 were genetically engineered as streptavidin fusions [single-chain Fv-streptavidin (scFvSA) fusion protein] for use in pretargeted radioimmunotherapy. The scFvSA constructs were expressed as soluble, tetrameric species in the periplasm of Escherichia coli. Expression levels were affected by the order of the variable regions and the length and composition of the single-chain Fv linker. The best expressor was obtained with the variable regions in the heavy chain-light chain configuration separated by a 25-mer Gly4Ser linker. This construct produced 250-300 mg of soluble, tetrameric fusion protein per liter of fermentor culture. The fusion protein (Mr 173,600) was purified from crude lysates by iminobiotin affinity chromatography with an overall yield of about 50% and was analyzed for functionality both in vitro and in vivo. Immunoreactivity of the scFvSA fusion protein and its nanomolar affinity to CD20-positive Ramos cells were comparable with the B9E9 monoclonal antibody. The fusion protein had a biotin dissociation rate identical to recombinant streptavidin and bound an average of 3.6 biotins/molecule of a possible 4 biotins/molecule. Labeled fusion protein cleared from the blood of BALB/c mice with a P half-life of about 16 h. In nude mice bearing Ramos xenografts, the fusion protein demonstrated sufficient tumor localization of functional streptavidin to enable efficient, tumor-specific targeting of a subsequently administered radionuclide-chelate/biotin molecule. These results suggest that large quantities of functional scFvSA can be produced for clinical testing as a therapy for non-Hodgkin's lymphoma." @default.
• W2165573190 created "2016-06-24" @default.
• W2165573190 creator A5000747443 @default.
• W2165573190 creator A5035987398 @default.
• W2165573190 creator A5048985521 @default.
• W2165573190 creator A5053467199 @default.
• W2165573190 creator A5063667737 @default.
• W2165573190 creator A5065600983 @default.
• W2165573190 creator A5076111835 @default.
• W2165573190 creator A5083041616 @default.
• W2165573190 date "2000-12-01" @default.
• W2165573190 modified "2023-09-23" @default.
• W2165573190 title "A tetravalent single-chain antibody-streptavidin fusion protein for pretargeted lymphoma therapy." @default.
• W2165573190 cites W1484092314 @default.
• W2165573190 cites W1512221515 @default.
• W2165573190 cites W1815017480 @default.
• W2165573190 cites W1858303686 @default.
• W2165573190 cites W1875396998 @default.
• W2165573190 cites W1963523261 @default.
• W2165573190 cites W1979223946 @default.
• W2165573190 cites W1988287443 @default.
• W2165573190 cites W1990229681 @default.
• W2165573190 cites W1996635945 @default.
• W2165573190 cites W1998622293 @default.
• W2165573190 cites W2004224153 @default.
• W2165573190 cites W2005986251 @default.
• W2165573190 cites W2006327509 @default.
• W2165573190 cites W2006968970 @default.
• W2165573190 cites W2007252162 @default.
• W2165573190 cites W2007734777 @default.
• W2165573190 cites W2009834607 @default.
• W2165573190 cites W2026036045 @default.
• W2165573190 cites W2058426435 @default.
• W2165573190 cites W2065307898 @default.
• W2165573190 cites W2078662180 @default.
• W2165573190 cites W2079876729 @default.
• W2165573190 cites W2086649261 @default.
• W2165573190 cites W2101108802 @default.
• W2165573190 cites W2135157531 @default.
• W2165573190 cites W2146102054 @default.
• W2165573190 cites W2320751441 @default.
• W2165573190 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11118050" @default.
• W2165573190 hasPublicationYear "2000" @default.
• W2165573190 type Work @default.
• W2165573190 sameAs 2165573190 @default.
• W2165573190 citedByCount "33" @default.
• W2165573190 countsByYear W21655731902012 @default.
• W2165573190 countsByYear W21655731902013 @default.
• W2165573190 countsByYear W21655731902014 @default.
• W2165573190 countsByYear W21655731902015 @default.
• W2165573190 countsByYear W21655731902016 @default.
• W2165573190 countsByYear W21655731902018 @default.
• W2165573190 countsByYear W21655731902019 @default.
• W2165573190 countsByYear W21655731902020 @default.
• W2165573190 countsByYear W21655731902022 @default.
• W2165573190 crossrefType "journal-article" @default.
• W2165573190 hasAuthorship W2165573190A5000747443 @default.
• W2165573190 hasAuthorship W2165573190A5035987398 @default.
• W2165573190 hasAuthorship W2165573190A5048985521 @default.
• W2165573190 hasAuthorship W2165573190A5053467199 @default.
• W2165573190 hasAuthorship W2165573190A5063667737 @default.
• W2165573190 hasAuthorship W2165573190A5065600983 @default.
• W2165573190 hasAuthorship W2165573190A5076111835 @default.
• W2165573190 hasAuthorship W2165573190A5083041616 @default.
• W2165573190 hasConcept C104317684 @default.
• W2165573190 hasConcept C111919701 @default.
• W2165573190 hasConcept C123894998 @default.
• W2165573190 hasConcept C145508813 @default.
• W2165573190 hasConcept C153911025 @default.
• W2165573190 hasConcept C159654299 @default.
• W2165573190 hasConcept C185592680 @default.
• W2165573190 hasConcept C203014093 @default.
• W2165573190 hasConcept C204628709 @default.
• W2165573190 hasConcept C2776146153 @default.
• W2165573190 hasConcept C2776190903 @default.
• W2165573190 hasConcept C2778204606 @default.
• W2165573190 hasConcept C2780557392 @default.
• W2165573190 hasConcept C36394416 @default.
• W2165573190 hasConcept C40767141 @default.
• W2165573190 hasConcept C41008148 @default.
• W2165573190 hasConcept C542903549 @default.
• W2165573190 hasConcept C55493867 @default.
• W2165573190 hasConcept C86803240 @default.
• W2165573190 hasConceptScore W2165573190C104317684 @default.
• W2165573190 hasConceptScore W2165573190C111919701 @default.
• W2165573190 hasConceptScore W2165573190C123894998 @default.
• W2165573190 hasConceptScore W2165573190C145508813 @default.
• W2165573190 hasConceptScore W2165573190C153911025 @default.
• W2165573190 hasConceptScore W2165573190C159654299 @default.
• W2165573190 hasConceptScore W2165573190C185592680 @default.
• W2165573190 hasConceptScore W2165573190C203014093 @default.
• W2165573190 hasConceptScore W2165573190C204628709 @default.
• W2165573190 hasConceptScore W2165573190C2776146153 @default.
• W2165573190 hasConceptScore W2165573190C2776190903 @default.
• W2165573190 hasConceptScore W2165573190C2778204606 @default.
• W2165573190 hasConceptScore W2165573190C2780557392 @default.
• W2165573190 hasConceptScore W2165573190C36394416 @default.
• W2165573190 hasConceptScore W2165573190C40767141 @default.

• next