FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2165589515> ?p ?o ?g. }

• W2165589515 endingPage "2554" @default.
• W2165589515 startingPage "2545" @default.
• W2165589515 abstract "TAP, the human homologue of the yeast protein Mex67p, has been proposed to serve a role in mRNA export in mammalian cells. We have examined the ability of TAP to mediate export of Rev response element (RRE)-containing human immunodeficiency virus (HIV) RNA, a well-characterized export substrate in mammalian cells. To do this, the TAP gene was fused in frame to either RevM10 or RevDelta78-79. These proteins are nonfunctional Rev mutant proteins that can bind to HIV RNA containing the RRE in vivo but are unable to mediate the export of this RNA to the cytoplasm. However, the fusion of TAP to either of these mutant proteins gave rise to chimeric proteins that were able to complement Rev function. Significantly, cotransfection with a vector expressing NXT1 (p15), an NTF2-related cellular factor that binds to TAP, led to dramatic enhancement of the ability of the chimeric proteins to mediate RNA export. Mutant-protein analysis demonstrated that the domain necessary for nuclear export mapped to the C-terminal region of TAP and required the domain that interacts with NXT1, as well as the region that has been shown to interact with nucleoporins. RevM10-TAP function was leptomycin B insensitive. In contrast, the function of this protein was inhibited by DeltaCAN, a protein consisting of part of the FG repeat domain of CAN/Nup214. These results show that TAP can complement Rev nuclear export signal function and redirect the export of intron-containing RNA to a CRM1-independent pathway. These experiments support the role of TAP as an RNA export factor in mammalian cells. In addition, they indicate that NXT1 serves as a crucial cellular cofactor in this process." @default.
• W2165589515 created "2016-06-24" @default.
• W2165589515 creator A5004155478 @default.
• W2165589515 creator A5034062115 @default.
• W2165589515 creator A5035608364 @default.
• W2165589515 creator A5045143510 @default.
• W2165589515 creator A5045703189 @default.
• W2165589515 creator A5047924377 @default.
• W2165589515 creator A5059118293 @default.
• W2165589515 creator A5088847670 @default.
• W2165589515 date "2001-04-01" @default.
• W2165589515 modified "2023-09-30" @default.
• W2165589515 title "NXT1 (p15) Is a Crucial Cellular Cofactor in TAP-Dependent Export of Intron-Containing RNA in Mammalian Cells" @default.
• W2165589515 cites W1510096565 @default.
• W2165589515 cites W1544851733 @default.
• W2165589515 cites W1567396361 @default.
• W2165589515 cites W1659684293 @default.
• W2165589515 cites W1792619367 @default.
• W2165589515 cites W1835940621 @default.
• W2165589515 cites W1867411047 @default.
• W2165589515 cites W1876741505 @default.
• W2165589515 cites W1904099063 @default.
• W2165589515 cites W1935409219 @default.
• W2165589515 cites W1964916693 @default.
• W2165589515 cites W1979763797 @default.
• W2165589515 cites W1981457569 @default.
• W2165589515 cites W1998162329 @default.
• W2165589515 cites W2003969944 @default.
• W2165589515 cites W2005855998 @default.
• W2165589515 cites W2006467763 @default.
• W2165589515 cites W2008922773 @default.
• W2165589515 cites W2008984155 @default.
• W2165589515 cites W2014876277 @default.
• W2165589515 cites W2018232440 @default.
• W2165589515 cites W2021397335 @default.
• W2165589515 cites W2032785100 @default.
• W2165589515 cites W2037534409 @default.
• W2165589515 cites W2040516035 @default.
• W2165589515 cites W2042170550 @default.
• W2165589515 cites W2043455507 @default.
• W2165589515 cites W2045845081 @default.
• W2165589515 cites W2050627047 @default.
• W2165589515 cites W2058054940 @default.
• W2165589515 cites W2060913542 @default.
• W2165589515 cites W2061184182 @default.
• W2165589515 cites W2062692712 @default.
• W2165589515 cites W2069490539 @default.
• W2165589515 cites W2071957053 @default.
• W2165589515 cites W2077430714 @default.
• W2165589515 cites W2084324934 @default.
• W2165589515 cites W2090038275 @default.
• W2165589515 cites W2091856409 @default.
• W2165589515 cites W2097752168 @default.
• W2165589515 cites W2098020712 @default.
• W2165589515 cites W2098441269 @default.
• W2165589515 cites W2099752605 @default.
• W2165589515 cites W2104148336 @default.
• W2165589515 cites W2109324124 @default.
• W2165589515 cites W2116797743 @default.
• W2165589515 cites W2117951174 @default.
• W2165589515 cites W2126182028 @default.
• W2165589515 cites W2127901995 @default.
• W2165589515 cites W2137393600 @default.
• W2165589515 cites W2137990405 @default.
• W2165589515 cites W2143538824 @default.
• W2165589515 cites W2144725211 @default.
• W2165589515 cites W2151298994 @default.
• W2165589515 cites W2153405631 @default.
• W2165589515 cites W2155085170 @default.
• W2165589515 cites W2158063520 @default.
• W2165589515 cites W2159391740 @default.
• W2165589515 cites W2163765191 @default.
• W2165589515 cites W2163828429 @default.
• W2165589515 cites W2170629529 @default.
• W2165589515 cites W2171258458 @default.
• W2165589515 cites W2171305498 @default.
• W2165589515 cites W2306779823 @default.
• W2165589515 cites W894556329 @default.
• W2165589515 doi "https://doi.org/10.1128/mcb.21.7.2545-2554.2001" @default.
• W2165589515 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/86886" @default.
• W2165589515 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11259602" @default.
• W2165589515 hasPublicationYear "2001" @default.
• W2165589515 type Work @default.
• W2165589515 sameAs 2165589515 @default.
• W2165589515 citedByCount "100" @default.
• W2165589515 countsByYear W21655895152012 @default.
• W2165589515 countsByYear W21655895152013 @default.
• W2165589515 countsByYear W21655895152014 @default.
• W2165589515 countsByYear W21655895152015 @default.
• W2165589515 countsByYear W21655895152016 @default.
• W2165589515 countsByYear W21655895152018 @default.
• W2165589515 countsByYear W21655895152019 @default.
• W2165589515 countsByYear W21655895152020 @default.
• W2165589515 countsByYear W21655895152021 @default.
• W2165589515 countsByYear W21655895152022 @default.
• W2165589515 crossrefType "journal-article" @default.
• W2165589515 hasAuthorship W2165589515A5004155478 @default.
• W2165589515 hasAuthorship W2165589515A5034062115 @default.

• next