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• W2165639925 abstract "Stimulated endothelial cells (EC) assume an activated phenotype with pro-inflammatory and prothrombotic features, requiring new gene and protein expression. New protein synthesis in activated EC is largely regulated by transcriptional events controlled by a variety of transcription factors. However, post-transcriptional control of gene expression also influences phenotype and allows the cell to alter protein expression in a faster and more direct way than is typically possible with transcriptional mechanisms. We sought to demonstrate that post-transcriptional control of gene expression occurs during EC activation. Using thrombin-activated EC and a high-throughput, microarray-based approach, we identified a number of gene products that may be regulated through post-transcriptional mechanisms, including the AP-1 transcription factor JunB. Using polysome profiling, cytoplasts and other standard cell biologic techniques, JunB is shown to be regulated at a post-transcriptional level during EC activation. In activated EC, the AP-1 transcription factor JunB, is regulated on a post-transcriptional level. Signal-dependent control of translation may regulate transcription factor expression and therefore, subsequent transcriptional events in stimulated EC. J. Cell. Biochem. 114: 1519–1528, 2013. © 2013 Wiley Periodicals, Inc." @default.
• W2165639925 created "2016-06-24" @default.
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• W2165639925 date "2013-05-09" @default.
• W2165639925 modified "2023-09-27" @default.
• W2165639925 title "Translational control of JunB, an AP-1 transcription factor, in activated human endothelial cells" @default.
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• W2165639925 doi "https://doi.org/10.1002/jcb.24493" @default.
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