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• W2165706180 startingPage "984" @default.
• W2165706180 abstract "Summary The three protozoan species C ryptosporidium parvum , C . meleagridis and C . hominis (phylum A picomplexa ) are enteric pathogens of humans. The former two species are zoonotic and the latter is thought to infect only humans. To better characterize the structure and transmission of natural and laboratory‐propagated isolates, we analyzed a collection of archived human and animal isolates of these three species by deep‐sequencing polymerase chain reaction products amplified from a polymorphic sequence on chromosome 1. Thousands of screened 200‐nucleotide sequences were analyzed to compare the diversity among samples, to assess the impact of laboratory propagation on population complexity and to identify taxonomically mixed isolates. Contrary to our expectation, repeated propagation in animals did not reduce intra‐isolate diversity nor was diversity associated with host species. Significantly, in most samples, sequences characteristic of a different species were identified. The presence of C . hominis alleles in C . parvum and C . meleagridis isolates confirms earlier reports of mixed isolates and raises the possibility that the host range of C . hominis is broader than typically assumed. In a genetically divergent isolate of C . parvum , a majority of sequences was found to be recombinant, suggesting that this genotype originated from a C . parvum × C . hominis recombination event." @default.
• W2165706180 created "2016-06-24" @default.
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• W2165706180 date "2014-04-02" @default.
• W2165706180 modified "2023-09-27" @default.
• W2165706180 title "Population structure of natural and propagated isolates of<i>C</i><i>ryptosporidium parvum</i>,<i>C</i>. <i>hominis</i>and<i>C</i>. <i>meleagridis</i>" @default.
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• W2165706180 doi "https://doi.org/10.1111/1462-2920.12447" @default.
• W2165706180 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24593863" @default.
• W2165706180 hasPublicationYear "2014" @default.
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