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- W2166012083 abstract "Two simple, sensitive and accurate spectrofluorimetric and spectrophotometric methods have been developed for the determination of sparfloxacin in bulk and pharmaceutical preparations. The proposed methods were based on oxidation of sparfloxacin with Ce (IV) in acidic medium. The spectrophotometric method involved the measurement of unconsumed Ce (IV) concentration at 315 nm. The spectrofluorimetric method based on the measurement of reduced fluorescent Ce (III) at 352 nm after excitation at 250 nm. Different variables affecting the reaction such as concentration and volume of cerium (IV), type and concentration of acidic medium, heating temperature and time were carefully studied and optimized. Under the optimum conditions, linear relationship in the range of 0.02-0.2 μg mL<sup>-1</sup> and 0.02-0.1 μg mL<sup>-1</sup> were obtained using spectrophotometric and spectrofluorimetric methods, respectively. No interferences were observed from the common formulations excipients present in the dosage form of the drug. The proposed methods were successfully applied to the analysis of the investigated drug in pure and pharmaceutical formulations with good accuracy and precision. The recovery percentage ranged from 93-102 ± 1.73-2.66%. The precision of the methods were good; RSD ≤ 2.55%." @default.
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- W2166012083 date "2017-10-12" @default.
- W2166012083 modified "2023-09-26" @default.
- W2166012083 title "Sensitive spectrofluorimetric and spectrophotometric methods for determination of sparfloxacin in pharmaceuticals" @default.
- W2166012083 doi "https://doi.org/10.29356/jmcs.v56i2.306" @default.
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