FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2166138466> ?p ?o ?g. }

• W2166138466 endingPage "144" @default.
• W2166138466 startingPage "135" @default.
• W2166138466 abstract "In the effort to produce proteins coded by diverse genomes, structural genomics projects often must express genes containing codons that are rare in the production strain. To address this problem, genes expressing tRNAs corresponding to those codons are typically coexpressed from a second plasmid in the host strain, or from genes incorporated into production plasmids. Here we describe the modification of a series of LIC pMCSG vectors currently used in the high-throughput (HTP) production of proteins to include crucial tRNA genes covering rare codons for Arg (AGG/AGA) and Ile (AUA). We also present variants of these new vectors that allow analysis of ligand binding or co-expression of multiple proteins introduced through two independent LIC steps. Additionally, to accommodate the cloning of multiple large proteins, the size of the plasmids was reduced by approximately one kilobase through the removal of non-essential DNA from the base vector. Production of proteins from core vectors of this series validated the desired enhanced capabilities: higher yields of proteins expressed from genes with rare codons occurred in most cases, biotinylated derivatives enabled detailed automated ligand binding analysis, and multiple proteins introduced by dual LIC cloning were expressed successfully and in near balanced stoichiometry, allowing tandem purification of interacting proteins." @default.
• W2166138466 created "2016-06-24" @default.
• W2166138466 creator A5015893701 @default.
• W2166138466 creator A5030976943 @default.
• W2166138466 creator A5053548487 @default.
• W2166138466 creator A5053702486 @default.
• W2166138466 creator A5085730573 @default.
• W2166138466 creator A5087916879 @default.
• W2166138466 date "2013-09-22" @default.
• W2166138466 modified "2023-10-14" @default.
• W2166138466 title "New LIC vectors for production of proteins from genes containing rare codons" @default.
• W2166138466 cites W1508587432 @default.
• W2166138466 cites W1509638340 @default.
• W2166138466 cites W1602519127 @default.
• W2166138466 cites W161956619 @default.
• W2166138466 cites W1799140735 @default.
• W2166138466 cites W1869516088 @default.
• W2166138466 cites W1966379061 @default.
• W2166138466 cites W1969243420 @default.
• W2166138466 cites W1970386045 @default.
• W2166138466 cites W1987868713 @default.
• W2166138466 cites W1993970200 @default.
• W2166138466 cites W1995134802 @default.
• W2166138466 cites W1998776029 @default.
• W2166138466 cites W2004275197 @default.
• W2166138466 cites W2019410656 @default.
• W2166138466 cites W2021422325 @default.
• W2166138466 cites W2025665147 @default.
• W2166138466 cites W2039377812 @default.
• W2166138466 cites W2039707307 @default.
• W2166138466 cites W2041602177 @default.
• W2166138466 cites W2044886732 @default.
• W2166138466 cites W2045808714 @default.
• W2166138466 cites W204911951 @default.
• W2166138466 cites W2051671456 @default.
• W2166138466 cites W2066076157 @default.
• W2166138466 cites W2069709525 @default.
• W2166138466 cites W2070781050 @default.
• W2166138466 cites W2071235304 @default.
• W2166138466 cites W2085541428 @default.
• W2166138466 cites W2105591215 @default.
• W2166138466 cites W2116595386 @default.
• W2166138466 cites W2122308238 @default.
• W2166138466 cites W2122609893 @default.
• W2166138466 cites W2123595241 @default.
• W2166138466 cites W2125569700 @default.
• W2166138466 cites W2127811150 @default.
• W2166138466 cites W2131864088 @default.
• W2166138466 cites W2142377280 @default.
• W2166138466 cites W2144146027 @default.
• W2166138466 cites W2160898059 @default.
• W2166138466 cites W2165217024 @default.
• W2166138466 cites W2165319386 @default.
• W2166138466 cites W2171892411 @default.
• W2166138466 cites W2407721199 @default.
• W2166138466 doi "https://doi.org/10.1007/s10969-013-9163-9" @default.
• W2166138466 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3933008" @default.
• W2166138466 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24057978" @default.
• W2166138466 hasPublicationYear "2013" @default.
• W2166138466 type Work @default.
• W2166138466 sameAs 2166138466 @default.
• W2166138466 citedByCount "51" @default.
• W2166138466 countsByYear W21661384662014 @default.
• W2166138466 countsByYear W21661384662015 @default.
• W2166138466 countsByYear W21661384662016 @default.
• W2166138466 countsByYear W21661384662017 @default.
• W2166138466 countsByYear W21661384662018 @default.
• W2166138466 countsByYear W21661384662019 @default.
• W2166138466 countsByYear W21661384662020 @default.
• W2166138466 countsByYear W21661384662021 @default.
• W2166138466 countsByYear W21661384662022 @default.
• W2166138466 countsByYear W21661384662023 @default.
• W2166138466 crossrefType "journal-article" @default.
• W2166138466 hasAuthorship W2166138466A5015893701 @default.
• W2166138466 hasAuthorship W2166138466A5030976943 @default.
• W2166138466 hasAuthorship W2166138466A5053548487 @default.
• W2166138466 hasAuthorship W2166138466A5053702486 @default.
• W2166138466 hasAuthorship W2166138466A5085730573 @default.
• W2166138466 hasAuthorship W2166138466A5087916879 @default.
• W2166138466 hasBestOaLocation W21661384662 @default.
• W2166138466 hasConcept C104317684 @default.
• W2166138466 hasConcept C107618985 @default.
• W2166138466 hasConcept C121050878 @default.
• W2166138466 hasConcept C141231307 @default.
• W2166138466 hasConcept C199360897 @default.
• W2166138466 hasConcept C22744801 @default.
• W2166138466 hasConcept C33161422 @default.
• W2166138466 hasConcept C40767141 @default.
• W2166138466 hasConcept C41008148 @default.
• W2166138466 hasConcept C54355233 @default.
• W2166138466 hasConcept C70721500 @default.
• W2166138466 hasConcept C86803240 @default.
• W2166138466 hasConceptScore W2166138466C104317684 @default.
• W2166138466 hasConceptScore W2166138466C107618985 @default.
• W2166138466 hasConceptScore W2166138466C121050878 @default.
• W2166138466 hasConceptScore W2166138466C141231307 @default.
• W2166138466 hasConceptScore W2166138466C199360897 @default.
• W2166138466 hasConceptScore W2166138466C22744801 @default.

• next