FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2166212912> ?p ?o ?g. }

• W2166212912 abstract "Retinal ganglion cell (RGC) loss is one of the earliest and most important cellular changes in glaucoma. The DARC (Detection of Apoptosing Retinal Cells) technology enables in vivo real-time non-invasive imaging of single apoptosing retinal cells in animal models of glaucoma and Alzheimer’s disease. To date, apoptosing RGCs imaged using DARC have been counted manually. This is time-consuming, labour-intensive, vulnerable to bias, and has considerable inter- and intra-operator variability. A semi-automated algorithm was developed which enabled automated identification of apoptosing RGCs labeled with fluorescent Annexin-5 on DARC images. Automated analysis included a pre-processing stage involving local-luminance and local-contrast “gain control”, a “blob analysis” step to differentiate between cells, vessels and noise, and a method to exclude non-cell structures using specific combined ‘size’ and ‘aspect’ ratio criteria. Apoptosing retinal cells were counted by 3 masked operators, generating ‘Gold-standard’ mean manual cell counts, and were also counted using the newly developed automated algorithm. Comparison between automated cell counts and the mean manual cell counts on 66 DARC images showed significant correlation between the two methods (Pearson’s correlation coefficient 0.978 (p < 0.001), R Squared = 0.956. The Intraclass correlation coefficient was 0.986 (95% CI 0.977-0.991, p < 0.001), and Cronbach’s alpha measure of consistency = 0.986, confirming excellent correlation and consistency. No significant difference (p = 0.922, 95% CI: −5.53 to 6.10) was detected between the cell counts of the two methods. The novel automated algorithm enabled accurate quantification of apoptosing RGCs that is highly comparable to manual counting, and appears to minimise operator-bias, whilst being both fast and reproducible. This may prove to be a valuable method of quantifying apoptosing retinal cells, with particular relevance to translation in the clinic, where a Phase I clinical trial of DARC in glaucoma patients is due to start shortly." @default.
• W2166212912 created "2016-06-24" @default.
• W2166212912 creator A5002447216 @default.
• W2166212912 creator A5019987116 @default.
• W2166212912 creator A5030736220 @default.
• W2166212912 creator A5037524372 @default.
• W2166212912 creator A5044451478 @default.
• W2166212912 creator A5051169186 @default.
• W2166212912 creator A5053241890 @default.
• W2166212912 creator A5060629377 @default.
• W2166212912 creator A5090014791 @default.
• W2166212912 creator A5091239431 @default.
• W2166212912 date "2014-06-05" @default.
• W2166212912 modified "2023-10-14" @default.
• W2166212912 title "A semi-automated technique for labeling and counting of apoptosing retinal cells" @default.
• W2166212912 cites W1501982791 @default.
• W2166212912 cites W1582288539 @default.
• W2166212912 cites W1853144523 @default.
• W2166212912 cites W1970446480 @default.
• W2166212912 cites W1970548437 @default.
• W2166212912 cites W1971686124 @default.
• W2166212912 cites W1978950608 @default.
• W2166212912 cites W1980468242 @default.
• W2166212912 cites W1990508893 @default.
• W2166212912 cites W2001231402 @default.
• W2166212912 cites W2001457150 @default.
• W2166212912 cites W2008035892 @default.
• W2166212912 cites W2025638475 @default.
• W2166212912 cites W2032440099 @default.
• W2166212912 cites W2032863582 @default.
• W2166212912 cites W2034921833 @default.
• W2166212912 cites W2037760632 @default.
• W2166212912 cites W2039873713 @default.
• W2166212912 cites W2042528850 @default.
• W2166212912 cites W2061994413 @default.
• W2166212912 cites W2065209139 @default.
• W2166212912 cites W2075725245 @default.
• W2166212912 cites W2081358037 @default.
• W2166212912 cites W2083438676 @default.
• W2166212912 cites W2093207055 @default.
• W2166212912 cites W2113143305 @default.
• W2166212912 cites W2114843352 @default.
• W2166212912 cites W2121134942 @default.
• W2166212912 cites W2137386887 @default.
• W2166212912 cites W2140571346 @default.
• W2166212912 cites W2142332605 @default.
• W2166212912 cites W2154917588 @default.
• W2166212912 cites W2156740003 @default.
• W2166212912 cites W2169736040 @default.
• W2166212912 cites W2170926992 @default.
• W2166212912 cites W2792448780 @default.
• W2166212912 cites W4249810852 @default.
• W2166212912 doi "https://doi.org/10.1186/1471-2105-15-169" @default.
• W2166212912 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4063694" @default.
• W2166212912 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24902592" @default.
• W2166212912 hasPublicationYear "2014" @default.
• W2166212912 type Work @default.
• W2166212912 sameAs 2166212912 @default.
• W2166212912 citedByCount "23" @default.
• W2166212912 countsByYear W21662129122014 @default.
• W2166212912 countsByYear W21662129122015 @default.
• W2166212912 countsByYear W21662129122016 @default.
• W2166212912 countsByYear W21662129122017 @default.
• W2166212912 countsByYear W21662129122019 @default.
• W2166212912 countsByYear W21662129122020 @default.
• W2166212912 countsByYear W21662129122022 @default.
• W2166212912 countsByYear W21662129122023 @default.
• W2166212912 crossrefType "journal-article" @default.
• W2166212912 hasAuthorship W2166212912A5002447216 @default.
• W2166212912 hasAuthorship W2166212912A5019987116 @default.
• W2166212912 hasAuthorship W2166212912A5030736220 @default.
• W2166212912 hasAuthorship W2166212912A5037524372 @default.
• W2166212912 hasAuthorship W2166212912A5044451478 @default.
• W2166212912 hasAuthorship W2166212912A5051169186 @default.
• W2166212912 hasAuthorship W2166212912A5053241890 @default.
• W2166212912 hasAuthorship W2166212912A5060629377 @default.
• W2166212912 hasAuthorship W2166212912A5090014791 @default.
• W2166212912 hasAuthorship W2166212912A5091239431 @default.
• W2166212912 hasBestOaLocation W21662129121 @default.
• W2166212912 hasConcept C104709138 @default.
• W2166212912 hasConcept C105795698 @default.
• W2166212912 hasConcept C118487528 @default.
• W2166212912 hasConcept C136229726 @default.
• W2166212912 hasConcept C142724271 @default.
• W2166212912 hasConcept C153180895 @default.
• W2166212912 hasConcept C154945302 @default.
• W2166212912 hasConcept C2777624874 @default.
• W2166212912 hasConcept C2778527774 @default.
• W2166212912 hasConcept C2780827179 @default.
• W2166212912 hasConcept C3019060180 @default.
• W2166212912 hasConcept C33923547 @default.
• W2166212912 hasConcept C40993552 @default.
• W2166212912 hasConcept C41008148 @default.
• W2166212912 hasConcept C55078378 @default.
• W2166212912 hasConcept C71924100 @default.
• W2166212912 hasConcept C9893847 @default.
• W2166212912 hasConceptScore W2166212912C104709138 @default.
• W2166212912 hasConceptScore W2166212912C105795698 @default.
• W2166212912 hasConceptScore W2166212912C118487528 @default.
• W2166212912 hasConceptScore W2166212912C136229726 @default.

• next