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- W2166283261 abstract "Cdk1 drives both mitotic entry and the metaphase-to-anaphase transition. Past work has shown that Wee1 inhibition of Cdk1 blocks mitotic entry. Here we show that the budding yeast Wee1 kinase, Swe1, also restrains the metaphase-to-anaphase transition by preventing Cdk1 phosphorylation and activation of the mitotic form of the anaphase-promoting complex/cyclosome (APCCdc20). Deletion of SWE1 or its opposing phosphatase MIH1 (the budding yeast cdc25+) altered the timing of anaphase onset, and activation of the Swe1-dependent morphogenesis checkpoint or overexpression of Swe1 blocked cells in metaphase with reduced APC activity in vivo and in vitro. The morphogenesis checkpoint also depended on Cdc55, a regulatory subunit of protein phosphatase 2A (PP2A). cdc55Δ checkpoint defects were rescued by mutating 12 Cdk1 phosphorylation sites on the APC, demonstrating that the APC is a target of this checkpoint. These data suggest a model in which stepwise activation of Cdk1 and inhibition of PP2ACdc55 triggers anaphase onset." @default.
- W2166283261 created "2016-06-24" @default.
- W2166283261 creator A5023802834 @default.
- W2166283261 creator A5030499014 @default.
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- W2166283261 creator A5070218585 @default.
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- W2166283261 creator A5085476804 @default.
- W2166283261 creator A5091372116 @default.
- W2166283261 date "2013-06-10" @default.
- W2166283261 modified "2023-09-26" @default.
- W2166283261 title "A Wee1 checkpoint inhibits anaphase onset" @default.
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