FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2166696244> ?p ?o ?g. }

• W2166696244 endingPage "2436" @default.
• W2166696244 startingPage "2429" @default.
• W2166696244 abstract "ABSTRACT Nitrilases are important in the biosphere as participants in synthesis and degradation pathways for naturally occurring, as well as xenobiotically derived, nitriles. Because of their inherent enantioselectivity, nitrilases are also attractive as mild, selective catalysts for setting chiral centers in fine chemical synthesis. Unfortunately, <20 nitrilases have been reported in the scientific and patent literature, and because of stability or specificity shortcomings, their utility has been largely unrealized. In this study, 137 unique nitrilases, discovered from screening of >600 biotope-specific environmental DNA (eDNA) libraries, were characterized. Using culture-independent means, phylogenetically diverse genomes were captured from entire biotopes, and their genes were expressed heterologously in a common cloning host. Nitrilase genes were targeted in a selection-based expression assay of clonal populations numbering 10 6 to 10 10 members per eDNA library. A phylogenetic analysis of the novel sequences discovered revealed the presence of at least five major sequence clades within the nitrilase subfamily. Using three nitrile substrates targeted for their potential in chiral pharmaceutical synthesis, the enzymes were characterized for substrate specificity and stereospecificity. A number of important correlations were found between sequence clades and the selective properties of these nitrilases. These enzymes, discovered using a high-throughput, culture-independent method, provide a catalytic toolbox for enantiospecific synthesis of a variety of carboxylic acid derivatives, as well as an intriguing library for evolutionary and structural analyses." @default.
• W2166696244 created "2016-06-24" @default.
• W2166696244 creator A5000536472 @default.
• W2166696244 creator A5002956075 @default.
• W2166696244 creator A5010080835 @default.
• W2166696244 creator A5012677528 @default.
• W2166696244 creator A5014459612 @default.
• W2166696244 creator A5020000818 @default.
• W2166696244 creator A5025643610 @default.
• W2166696244 creator A5035423122 @default.
• W2166696244 creator A5035660803 @default.
• W2166696244 creator A5040509630 @default.
• W2166696244 creator A5046474284 @default.
• W2166696244 creator A5048218996 @default.
• W2166696244 creator A5054837771 @default.
• W2166696244 creator A5061736733 @default.
• W2166696244 creator A5067855504 @default.
• W2166696244 creator A5069664761 @default.
• W2166696244 creator A5070845405 @default.
• W2166696244 creator A5071066961 @default.
• W2166696244 creator A5072219555 @default.
• W2166696244 creator A5077749430 @default.
• W2166696244 creator A5083555185 @default.
• W2166696244 date "2004-04-01" @default.
• W2166696244 modified "2023-09-26" @default.
• W2166696244 title "Exploring Nitrilase Sequence Space for Enantioselective Catalysis" @default.
• W2166696244 cites W1974470318 @default.
• W2166696244 cites W1997954108 @default.
• W2166696244 cites W2010180565 @default.
• W2166696244 cites W2013776698 @default.
• W2166696244 cites W2025525785 @default.
• W2166696244 cites W2027152448 @default.
• W2166696244 cites W2027168767 @default.
• W2166696244 cites W2028316082 @default.
• W2166696244 cites W2035847135 @default.
• W2166696244 cites W2042896521 @default.
• W2166696244 cites W2052357315 @default.
• W2166696244 cites W2056702887 @default.
• W2166696244 cites W2068687524 @default.
• W2166696244 cites W2069516907 @default.
• W2166696244 cites W2071086763 @default.
• W2166696244 cites W2074122548 @default.
• W2166696244 cites W2094694540 @default.
• W2166696244 cites W2110282379 @default.
• W2166696244 cites W2122084702 @default.
• W2166696244 cites W2131988453 @default.
• W2166696244 cites W2141212142 @default.
• W2166696244 cites W2146386873 @default.
• W2166696244 cites W2149863305 @default.
• W2166696244 cites W2154998149 @default.
• W2166696244 cites W2159869703 @default.
• W2166696244 cites W2161444534 @default.
• W2166696244 cites W2162957773 @default.
• W2166696244 cites W588500352 @default.
• W2166696244 doi "https://doi.org/10.1128/aem.70.4.2429-2436.2004" @default.
• W2166696244 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/383143" @default.
• W2166696244 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15066841" @default.
• W2166696244 hasPublicationYear "2004" @default.
• W2166696244 type Work @default.
• W2166696244 sameAs 2166696244 @default.
• W2166696244 citedByCount "210" @default.
• W2166696244 countsByYear W21666962442012 @default.
• W2166696244 countsByYear W21666962442013 @default.
• W2166696244 countsByYear W21666962442014 @default.
• W2166696244 countsByYear W21666962442015 @default.
• W2166696244 countsByYear W21666962442016 @default.
• W2166696244 countsByYear W21666962442017 @default.
• W2166696244 countsByYear W21666962442018 @default.
• W2166696244 countsByYear W21666962442019 @default.
• W2166696244 countsByYear W21666962442020 @default.
• W2166696244 countsByYear W21666962442021 @default.
• W2166696244 countsByYear W21666962442022 @default.
• W2166696244 countsByYear W21666962442023 @default.
• W2166696244 crossrefType "journal-article" @default.
• W2166696244 hasAuthorship W2166696244A5000536472 @default.
• W2166696244 hasAuthorship W2166696244A5002956075 @default.
• W2166696244 hasAuthorship W2166696244A5010080835 @default.
• W2166696244 hasAuthorship W2166696244A5012677528 @default.
• W2166696244 hasAuthorship W2166696244A5014459612 @default.
• W2166696244 hasAuthorship W2166696244A5020000818 @default.
• W2166696244 hasAuthorship W2166696244A5025643610 @default.
• W2166696244 hasAuthorship W2166696244A5035423122 @default.
• W2166696244 hasAuthorship W2166696244A5035660803 @default.
• W2166696244 hasAuthorship W2166696244A5040509630 @default.
• W2166696244 hasAuthorship W2166696244A5046474284 @default.
• W2166696244 hasAuthorship W2166696244A5048218996 @default.
• W2166696244 hasAuthorship W2166696244A5054837771 @default.
• W2166696244 hasAuthorship W2166696244A5061736733 @default.
• W2166696244 hasAuthorship W2166696244A5067855504 @default.
• W2166696244 hasAuthorship W2166696244A5069664761 @default.
• W2166696244 hasAuthorship W2166696244A5070845405 @default.
• W2166696244 hasAuthorship W2166696244A5071066961 @default.
• W2166696244 hasAuthorship W2166696244A5072219555 @default.
• W2166696244 hasAuthorship W2166696244A5077749430 @default.
• W2166696244 hasAuthorship W2166696244A5083555185 @default.
• W2166696244 hasBestOaLocation W21666962442 @default.
• W2166696244 hasConcept C104317684 @default.
• W2166696244 hasConcept C130217890 @default.

• next