FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2166816916> ?p ?o ?g. }

• W2166816916 endingPage "291" @default.
• W2166816916 startingPage "279" @default.
• W2166816916 abstract "The crystal structures of the catalytic domain (DeltaN1-102/DeltaC428-452) of human phenylalanine hydroxylase (hPheOH) in its catalytically competent Fe(II) form and binary complex with the reduced pterin cofactor 6(R)-L-erythro-5,6,7,8-tetrahydrobiopterin (BH4) have been determined to 1.7 and 1.5 A, respectively. When compared with the structures reported for various catalytically inactive Fe(III) forms, several important differences have been observed, notably at the active site. Thus, the non-liganded hPheOH-Fe(II) structure revealed well defined electron density for only one of the three water molecules reported to be coordinated to the iron in the high-spin Fe(III) form, as well as poor electron density for parts of the coordinating side-chain of Glu330. The reduced cofactor (BH4), which adopts the expected half-semi chair conformation, is bound in the second coordination sphere of the catalytic iron with a C4a-iron distance of 5.9 A. BH4 binds at the same site as L-erythro-7,8-dihydrobiopterin (BH2) in the binary hPheOH-Fe(III)-BH2 complex forming an aromatic pi-stacking interaction with Phe254 and a network of hydrogen bonds. However, compared to that structure the pterin ring is displaced about 0.5 A and rotated about 10 degrees, and the torsion angle between the hydroxyl groups of the cofactor in the dihydroxypropyl side-chain has changed by approximately 120 degrees enabling O2' to make a strong hydrogen bond (2.4 A) with the side-chain oxygen of Ser251. Carbon atoms in the dihydroxypropyl side-chain make several hydrophobic contacts with the protein. The iron is six-coordinated in the binary complex, but the overall coordination geometry is slightly different from that of the Fe(III) form. Most important was the finding that the binding of BH4 causes the Glu330 ligand to change its coordination to the iron when comparing with non-liganded hPheOH-Fe(III) and the binary hPheOH-Fe(III)-BH2 complex." @default.
• W2166816916 created "2016-06-24" @default.
• W2166816916 creator A5022592678 @default.
• W2166816916 creator A5046775739 @default.
• W2166816916 creator A5055079299 @default.
• W2166816916 date "2001-11-01" @default.
• W2166816916 modified "2023-09-27" @default.
• W2166816916 title "High resolution crystal structures of the catalytic domain of human phenylalanine hydroxylase in its catalytically active Fe(II) form and binary complex with tetrahydrobiopterin" @default.
• W2166816916 cites W1496084247 @default.
• W2166816916 cites W1518494269 @default.
• W2166816916 cites W1535332154 @default.
• W2166816916 cites W1539796472 @default.
• W2166816916 cites W1545811790 @default.
• W2166816916 cites W1546896179 @default.
• W2166816916 cites W1556966074 @default.
• W2166816916 cites W1631593954 @default.
• W2166816916 cites W1710565104 @default.
• W2166816916 cites W1816125786 @default.
• W2166816916 cites W1868339174 @default.
• W2166816916 cites W1931119182 @default.
• W2166816916 cites W1967070015 @default.
• W2166816916 cites W1974443665 @default.
• W2166816916 cites W1980467950 @default.
• W2166816916 cites W1986191025 @default.
• W2166816916 cites W1987171585 @default.
• W2166816916 cites W1993404146 @default.
• W2166816916 cites W1995017064 @default.
• W2166816916 cites W1999646284 @default.
• W2166816916 cites W2002248339 @default.
• W2166816916 cites W2009000124 @default.
• W2166816916 cites W2013083986 @default.
• W2166816916 cites W2023348316 @default.
• W2166816916 cites W2024610281 @default.
• W2166816916 cites W2025916627 @default.
• W2166816916 cites W2027249579 @default.
• W2166816916 cites W2028231353 @default.
• W2166816916 cites W2030687437 @default.
• W2166816916 cites W2042987185 @default.
• W2166816916 cites W2046036275 @default.
• W2166816916 cites W2046291539 @default.
• W2166816916 cites W2049548535 @default.
• W2166816916 cites W2049972320 @default.
• W2166816916 cites W2055196039 @default.
• W2166816916 cites W2056467903 @default.
• W2166816916 cites W2060426883 @default.
• W2166816916 cites W2064505512 @default.
• W2166816916 cites W2065921399 @default.
• W2166816916 cites W2066589965 @default.
• W2166816916 cites W2067614539 @default.
• W2166816916 cites W2069041654 @default.
• W2166816916 cites W2076727938 @default.
• W2166816916 cites W2081912151 @default.
• W2166816916 cites W2082608486 @default.
• W2166816916 cites W2086957099 @default.
• W2166816916 cites W2087661270 @default.
• W2166816916 cites W2089511067 @default.
• W2166816916 cites W2091086834 @default.
• W2166816916 cites W2106776097 @default.
• W2166816916 cites W2117560166 @default.
• W2166816916 cites W2151207180 @default.
• W2166816916 cites W2322686317 @default.
• W2166816916 cites W2951583208 @default.
• W2166816916 cites W581857 @default.
• W2166816916 doi "https://doi.org/10.1006/jmbi.2001.5061" @default.
• W2166816916 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11718561" @default.
• W2166816916 hasPublicationYear "2001" @default.
• W2166816916 type Work @default.
• W2166816916 sameAs 2166816916 @default.
• W2166816916 citedByCount "100" @default.
• W2166816916 countsByYear W21668169162012 @default.
• W2166816916 countsByYear W21668169162013 @default.
• W2166816916 countsByYear W21668169162014 @default.
• W2166816916 countsByYear W21668169162015 @default.
• W2166816916 countsByYear W21668169162016 @default.
• W2166816916 countsByYear W21668169162017 @default.
• W2166816916 countsByYear W21668169162018 @default.
• W2166816916 countsByYear W21668169162019 @default.
• W2166816916 countsByYear W21668169162020 @default.
• W2166816916 countsByYear W21668169162021 @default.
• W2166816916 countsByYear W21668169162022 @default.
• W2166816916 countsByYear W21668169162023 @default.
• W2166816916 crossrefType "journal-article" @default.
• W2166816916 hasAuthorship W2166816916A5022592678 @default.
• W2166816916 hasAuthorship W2166816916A5046775739 @default.
• W2166816916 hasAuthorship W2166816916A5055079299 @default.
• W2166816916 hasConcept C112887158 @default.
• W2166816916 hasConcept C115624301 @default.
• W2166816916 hasConcept C161790260 @default.
• W2166816916 hasConcept C178790620 @default.
• W2166816916 hasConcept C181199279 @default.
• W2166816916 hasConcept C185592680 @default.
• W2166816916 hasConcept C197957613 @default.
• W2166816916 hasConcept C204921945 @default.
• W2166816916 hasConcept C207009774 @default.
• W2166816916 hasConcept C2776055822 @default.
• W2166816916 hasConcept C2776145790 @default.
• W2166816916 hasConcept C2777431362 @default.
• W2166816916 hasConcept C32909587 @default.

• next