FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2166908425> ?p ?o ?g. }

• W2166908425 endingPage "954" @default.
• W2166908425 startingPage "947" @default.
• W2166908425 abstract "Abstract Background: Maternal serum concentrations of pregnancy-associated plasma protein-A (PAPP-A, pappalysin-1, EC 3.4.24.79) are used to predict the occurrence of Down syndrome. In pregnancy, PAPP-A primarily circulates as a covalent 2:2 complex with the proform of eosinophil major basic protein (proMBP), which inhibits the proteolytic activity of PAPP-A. At term, however, ∼1% of PAPP-A exists as an active, uncomplexed dimer with proteolytic activity directed specifically toward insulin-like growth factor binding protein (IGFBP)-4 and IGFBP-5. No assays have been developed that allow quantification of PAPP-A proteolytic activity. Methods: We developed a sensitive and specific immunocapture assay for PAPP-A activity based on intramolecular quenched fluorescence. We used a 26-residue synthetic peptide derived from IGFBP-4 in which specific positions on each side of the PAPP-A cleavage site were substituted with 3-nitrotyrosine and o-aminobenzoic acid. Results: The assay detected the activity of recombinant PAPP-A as well as PAPP-A in serum samples from pregnant women. The limit of detection (mean signal of blank plus 3 SD) of the active PAPP-A subunit was 13 pmol/L, and the intra- and interassay CVs were &lt;10% and &lt;15%, respectively. Interestingly, the fraction of active PAPP-A decreased gradually from week 7 to week 19 of pregnancy. Conclusions: This method allows the measurement of PAPP-A enzymatic activity and because of its specificity it is relevant to the study of the biological function of PAPP-A. The method may also be useful in the diagnosis of pregnancy disorders." @default.
• W2166908425 created "2016-06-24" @default.
• W2166908425 creator A5025202919 @default.
• W2166908425 creator A5041160849 @default.
• W2166908425 creator A5079927114 @default.
• W2166908425 date "2007-05-01" @default.
• W2166908425 modified "2023-09-27" @default.
• W2166908425 title "Quantification of Proteolytically Active Pregnancy-Associated Plasma Protein-A with an Assay Based on Quenched Fluorescence" @default.
• W2166908425 cites W1731510796 @default.
• W2166908425 cites W1967882786 @default.
• W2166908425 cites W1976299678 @default.
• W2166908425 cites W1979741190 @default.
• W2166908425 cites W1989574221 @default.
• W2166908425 cites W2012505775 @default.
• W2166908425 cites W2015840376 @default.
• W2166908425 cites W2018264909 @default.
• W2166908425 cites W2036362928 @default.
• W2166908425 cites W2036997531 @default.
• W2166908425 cites W2040004801 @default.
• W2166908425 cites W2042693437 @default.
• W2166908425 cites W2045353567 @default.
• W2166908425 cites W2047829740 @default.
• W2166908425 cites W2048659949 @default.
• W2166908425 cites W2051839343 @default.
• W2166908425 cites W2061184447 @default.
• W2166908425 cites W2072514327 @default.
• W2166908425 cites W2095334858 @default.
• W2166908425 cites W2107585022 @default.
• W2166908425 cites W2117315526 @default.
• W2166908425 cites W2125459996 @default.
• W2166908425 cites W2134216947 @default.
• W2166908425 cites W2140868727 @default.
• W2166908425 cites W2145894739 @default.
• W2166908425 cites W2157442770 @default.
• W2166908425 cites W2165219224 @default.
• W2166908425 cites W4245595653 @default.
• W2166908425 cites W4253913636 @default.
• W2166908425 cites W62299279 @default.
• W2166908425 doi "https://doi.org/10.1373/clinchem.2006.080614" @default.
• W2166908425 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17363418" @default.
• W2166908425 hasPublicationYear "2007" @default.
• W2166908425 type Work @default.
• W2166908425 sameAs 2166908425 @default.
• W2166908425 citedByCount "33" @default.
• W2166908425 countsByYear W21669084252012 @default.
• W2166908425 countsByYear W21669084252013 @default.
• W2166908425 countsByYear W21669084252014 @default.
• W2166908425 countsByYear W21669084252015 @default.
• W2166908425 countsByYear W21669084252017 @default.
• W2166908425 countsByYear W21669084252018 @default.
• W2166908425 countsByYear W21669084252019 @default.
• W2166908425 countsByYear W21669084252020 @default.
• W2166908425 countsByYear W21669084252021 @default.
• W2166908425 countsByYear W21669084252022 @default.
• W2166908425 countsByYear W21669084252023 @default.
• W2166908425 crossrefType "journal-article" @default.
• W2166908425 hasAuthorship W2166908425A5025202919 @default.
• W2166908425 hasAuthorship W2166908425A5041160849 @default.
• W2166908425 hasAuthorship W2166908425A5079927114 @default.
• W2166908425 hasConcept C104317684 @default.
• W2166908425 hasConcept C153911025 @default.
• W2166908425 hasConcept C172680121 @default.
• W2166908425 hasConcept C181199279 @default.
• W2166908425 hasConcept C185592680 @default.
• W2166908425 hasConcept C2778014424 @default.
• W2166908425 hasConcept C2779234561 @default.
• W2166908425 hasConcept C2779281246 @default.
• W2166908425 hasConcept C3019223276 @default.
• W2166908425 hasConcept C3019714739 @default.
• W2166908425 hasConcept C40767141 @default.
• W2166908425 hasConcept C54355233 @default.
• W2166908425 hasConcept C55493867 @default.
• W2166908425 hasConcept C86803240 @default.
• W2166908425 hasConceptScore W2166908425C104317684 @default.
• W2166908425 hasConceptScore W2166908425C153911025 @default.
• W2166908425 hasConceptScore W2166908425C172680121 @default.
• W2166908425 hasConceptScore W2166908425C181199279 @default.
• W2166908425 hasConceptScore W2166908425C185592680 @default.
• W2166908425 hasConceptScore W2166908425C2778014424 @default.
• W2166908425 hasConceptScore W2166908425C2779234561 @default.
• W2166908425 hasConceptScore W2166908425C2779281246 @default.
• W2166908425 hasConceptScore W2166908425C3019223276 @default.
• W2166908425 hasConceptScore W2166908425C3019714739 @default.
• W2166908425 hasConceptScore W2166908425C40767141 @default.
• W2166908425 hasConceptScore W2166908425C54355233 @default.
• W2166908425 hasConceptScore W2166908425C55493867 @default.
• W2166908425 hasConceptScore W2166908425C86803240 @default.
• W2166908425 hasIssue "5" @default.
• W2166908425 hasLocation W21669084251 @default.
• W2166908425 hasLocation W21669084252 @default.
• W2166908425 hasOpenAccess W2166908425 @default.
• W2166908425 hasPrimaryLocation W21669084251 @default.
• W2166908425 hasRelatedWork W2006771406 @default.
• W2166908425 hasRelatedWork W2016628627 @default.
• W2166908425 hasRelatedWork W2029274884 @default.
• W2166908425 hasRelatedWork W2045482490 @default.
• W2166908425 hasRelatedWork W2057361610 @default.
• W2166908425 hasRelatedWork W2064042198 @default.

• next