FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2167232017> ?p ?o ?g. }

• W2167232017 endingPage "1420" @default.
• W2167232017 startingPage "1409" @default.
• W2167232017 abstract "We demonstrate here that CD59, an inhibitor of the membrane attack complex (MAC) of the complement system, is present in cell-free seminal plasma (SP) at a concentration of at least 20 micrograms/ml. Analyses by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, Western blotting, and Edman degradation indicated that this protein, SP CD59, was similar, if not identical, to CD59 isolated from erythrocyte (E) membranes (E CD59). Like purified E CD59, SP CD59 also possesses a glycosyl phosphatidyl inositol (GPI) anchor and incorporates into the membranes of heterologous cells where it inhibits lysis by the human MAC. This phenomenon could be demonstrated not only if cells were incubated with purified SP CD59 but also if unfractionated SP were used. Further, CD59 in unfractionated SP bound to washed spermatozoa, increasing their membrane content of the protein. The mechanism by which this protein retains its GPI anchor while apparently present in the fluid phase is of interest and was further investigated. Using the techniques of high-speed centrifugation, fast performance liquid chromatography fractionation, and electron microscopy, we found that all detectable SP CD59 was associated with vesicular extracellular organelles. These organelles, named prostasomes, were previously known to be present in SP and to interact with spermatozoa, although their function was uncertain. Interaction of heterologous E with prostasomes rendered the cells more resistant to lysis by human MACs. We propose that these organelles represent a pool of CD59 from which protein lost from spermatozoa, perhaps as a result of low level complement attack or of normal membrane turnover, can be replenished." @default.
• W2167232017 created "2016-06-24" @default.
• W2167232017 creator A5000291728 @default.
• W2167232017 creator A5008491017 @default.
• W2167232017 creator A5071126679 @default.
• W2167232017 creator A5071858916 @default.
• W2167232017 creator A5078499434 @default.
• W2167232017 creator A5081728604 @default.
• W2167232017 creator A5090102833 @default.
• W2167232017 date "1993-05-01" @default.
• W2167232017 modified "2023-10-06" @default.
• W2167232017 title "Physiologic relevance of the membrane attack complex inhibitory protein CD59 in human seminal plasma: CD59 is present on extracellular organelles (prostasomes), binds cell membranes, and inhibits complement-mediated lysis." @default.
• W2167232017 cites W137654459 @default.
• W2167232017 cites W142540160 @default.
• W2167232017 cites W1492987806 @default.
• W2167232017 cites W153373365 @default.
• W2167232017 cites W1580446023 @default.
• W2167232017 cites W1915536483 @default.
• W2167232017 cites W1949772429 @default.
• W2167232017 cites W1967853405 @default.
• W2167232017 cites W1977356373 @default.
• W2167232017 cites W1980009977 @default.
• W2167232017 cites W1985333754 @default.
• W2167232017 cites W1995640513 @default.
• W2167232017 cites W2001664656 @default.
• W2167232017 cites W2010096155 @default.
• W2167232017 cites W2012252491 @default.
• W2167232017 cites W2018289835 @default.
• W2167232017 cites W2019906171 @default.
• W2167232017 cites W2040378802 @default.
• W2167232017 cites W2065597712 @default.
• W2167232017 cites W206830091 @default.
• W2167232017 cites W2069444054 @default.
• W2167232017 cites W2081588732 @default.
• W2167232017 cites W2088147479 @default.
• W2167232017 cites W2096436192 @default.
• W2167232017 cites W2100772466 @default.
• W2167232017 cites W2100837269 @default.
• W2167232017 cites W2104199760 @default.
• W2167232017 cites W2133388060 @default.
• W2167232017 cites W2167539457 @default.
• W2167232017 cites W2235739349 @default.
• W2167232017 cites W226817784 @default.
• W2167232017 cites W2271388588 @default.
• W2167232017 cites W2338379496 @default.
• W2167232017 cites W2408577479 @default.
• W2167232017 cites W2410476973 @default.
• W2167232017 doi "https://doi.org/10.1084/jem.177.5.1409" @default.
• W2167232017 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2191001" @default.
• W2167232017 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7683035" @default.
• W2167232017 hasPublicationYear "1993" @default.
• W2167232017 type Work @default.
• W2167232017 sameAs 2167232017 @default.
• W2167232017 citedByCount "187" @default.
• W2167232017 countsByYear W21672320172012 @default.
• W2167232017 countsByYear W21672320172013 @default.
• W2167232017 countsByYear W21672320172014 @default.
• W2167232017 countsByYear W21672320172015 @default.
• W2167232017 countsByYear W21672320172016 @default.
• W2167232017 countsByYear W21672320172017 @default.
• W2167232017 countsByYear W21672320172018 @default.
• W2167232017 countsByYear W21672320172019 @default.
• W2167232017 countsByYear W21672320172020 @default.
• W2167232017 countsByYear W21672320172021 @default.
• W2167232017 countsByYear W21672320172022 @default.
• W2167232017 countsByYear W21672320172023 @default.
• W2167232017 crossrefType "journal-article" @default.
• W2167232017 hasAuthorship W2167232017A5000291728 @default.
• W2167232017 hasAuthorship W2167232017A5008491017 @default.
• W2167232017 hasAuthorship W2167232017A5071126679 @default.
• W2167232017 hasAuthorship W2167232017A5071858916 @default.
• W2167232017 hasAuthorship W2167232017A5078499434 @default.
• W2167232017 hasAuthorship W2167232017A5081728604 @default.
• W2167232017 hasAuthorship W2167232017A5090102833 @default.
• W2167232017 hasBestOaLocation W21672320171 @default.
• W2167232017 hasConcept C108625454 @default.
• W2167232017 hasConcept C111684460 @default.
• W2167232017 hasConcept C144647389 @default.
• W2167232017 hasConcept C153911025 @default.
• W2167232017 hasConcept C168240541 @default.
• W2167232017 hasConcept C184404459 @default.
• W2167232017 hasConcept C203014093 @default.
• W2167232017 hasConcept C2777459641 @default.
• W2167232017 hasConcept C28406088 @default.
• W2167232017 hasConcept C41625074 @default.
• W2167232017 hasConcept C55493867 @default.
• W2167232017 hasConcept C57409179 @default.
• W2167232017 hasConcept C79196540 @default.
• W2167232017 hasConcept C8487232 @default.
• W2167232017 hasConcept C86803240 @default.
• W2167232017 hasConcept C8891405 @default.
• W2167232017 hasConcept C95444343 @default.
• W2167232017 hasConceptScore W2167232017C108625454 @default.
• W2167232017 hasConceptScore W2167232017C111684460 @default.
• W2167232017 hasConceptScore W2167232017C144647389 @default.
• W2167232017 hasConceptScore W2167232017C153911025 @default.
• W2167232017 hasConceptScore W2167232017C168240541 @default.
• W2167232017 hasConceptScore W2167232017C184404459 @default.

• next