Matches in SemOpenAlex for { <https://semopenalex.org/work/W2167322592> ?p ?o ?g. }
- W2167322592 endingPage "568" @default.
- W2167322592 startingPage "557" @default.
- W2167322592 abstract "ABSTRACT Cryptococcus neoformans is a human fungal pathogen that often causes lung and brain infections in immunocompromised patients, with a high fatality rate. Our previous results showed that an F-box protein, Fbp1, is essential for Cryptococcus virulence independent of the classical virulence factors, suggesting a novel virulence control mechanism. In this study, we show that Fbp1 is part of the ubiquitin-proteasome system, and we further investigated the mechanism of Fbp1 function during infection. Time course studies revealed that the fbp1 Δ mutant causes little damage in the infected lung and that the fungal burden in the lung remains at a low but persistent level throughout infection. The fbp1 Δ mutant cannot disseminate to other organs following pulmonary infection in the murine inhalation model of cryptococcosis but still causes brain infection in a murine intravenous injection model, suggesting that the block of dissemination of the fbp1 Δ mutant is due to its inability to leave the lung. The fbp1 Δ mutant showed a defect in intracellular proliferation after phagocytosis in a Cryptococcus -macrophage interaction assay, which likely contributes to its virulence attenuation. To elucidate the molecular basis of the SCF(Fbp1) E3 ligase function, we analyzed potential Fbp1 substrates based on proteomic approaches combined with phenotypic analysis. One substrate, the inositol phosphosphingolipid-phospholipase C1 (Isc1), is required for fungal survival inside macrophage cells, which is consistent with the role of Fbp1 in regulating Cryptococcus -macrophage interaction and fungal virulence. Our results thus reveal a new determinant of fungal virulence that involves the posttranslational regulation of inositol sphingolipid biosynthesis." @default.
- W2167322592 created "2016-06-24" @default.
- W2167322592 creator A5086256968 @default.
- W2167322592 creator A5087137800 @default.
- W2167322592 date "2014-02-01" @default.
- W2167322592 modified "2023-09-30" @default.
- W2167322592 title "Fbp1-Mediated Ubiquitin-Proteasome Pathway Controls Cryptococcus neoformans Virulence by Regulating Fungal Intracellular Growth in Macrophages" @default.
- W2167322592 cites W1598369770 @default.
- W2167322592 cites W1598765630 @default.
- W2167322592 cites W1963530556 @default.
- W2167322592 cites W1964444162 @default.
- W2167322592 cites W1965181237 @default.
- W2167322592 cites W1966034049 @default.
- W2167322592 cites W1970409736 @default.
- W2167322592 cites W1978726512 @default.
- W2167322592 cites W1981849277 @default.
- W2167322592 cites W1982958726 @default.
- W2167322592 cites W1983747336 @default.
- W2167322592 cites W1984649251 @default.
- W2167322592 cites W1996936752 @default.
- W2167322592 cites W1999259744 @default.
- W2167322592 cites W2001430852 @default.
- W2167322592 cites W2009456747 @default.
- W2167322592 cites W2010601153 @default.
- W2167322592 cites W2011498074 @default.
- W2167322592 cites W2018552263 @default.
- W2167322592 cites W2022551715 @default.
- W2167322592 cites W2023100224 @default.
- W2167322592 cites W2042092509 @default.
- W2167322592 cites W2044053799 @default.
- W2167322592 cites W2046208000 @default.
- W2167322592 cites W2059418838 @default.
- W2167322592 cites W2065557130 @default.
- W2167322592 cites W2068100481 @default.
- W2167322592 cites W2070511399 @default.
- W2167322592 cites W2070916151 @default.
- W2167322592 cites W2073897065 @default.
- W2167322592 cites W2075124527 @default.
- W2167322592 cites W2079513681 @default.
- W2167322592 cites W2094067639 @default.
- W2167322592 cites W2095699986 @default.
- W2167322592 cites W2096323533 @default.
- W2167322592 cites W2098195961 @default.
- W2167322592 cites W2098472248 @default.
- W2167322592 cites W2099573346 @default.
- W2167322592 cites W2099993657 @default.
- W2167322592 cites W2106906679 @default.
- W2167322592 cites W2107277218 @default.
- W2167322592 cites W2114778619 @default.
- W2167322592 cites W2115040899 @default.
- W2167322592 cites W2116627896 @default.
- W2167322592 cites W2117474926 @default.
- W2167322592 cites W2117680176 @default.
- W2167322592 cites W2124600371 @default.
- W2167322592 cites W2134481201 @default.
- W2167322592 cites W2136260545 @default.
- W2167322592 cites W2137037523 @default.
- W2167322592 cites W2139015487 @default.
- W2167322592 cites W2142145420 @default.
- W2167322592 cites W2143733560 @default.
- W2167322592 cites W2144114582 @default.
- W2167322592 cites W2144306209 @default.
- W2167322592 cites W2146113706 @default.
- W2167322592 cites W2147478719 @default.
- W2167322592 cites W2151840748 @default.
- W2167322592 cites W2156080242 @default.
- W2167322592 cites W2166751413 @default.
- W2167322592 cites W4210875411 @default.
- W2167322592 cites W4211124143 @default.
- W2167322592 cites W4234195097 @default.
- W2167322592 doi "https://doi.org/10.1128/iai.00994-13" @default.
- W2167322592 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3911387" @default.
- W2167322592 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24478071" @default.
- W2167322592 hasPublicationYear "2014" @default.
- W2167322592 type Work @default.
- W2167322592 sameAs 2167322592 @default.
- W2167322592 citedByCount "39" @default.
- W2167322592 countsByYear W21673225922014 @default.
- W2167322592 countsByYear W21673225922015 @default.
- W2167322592 countsByYear W21673225922016 @default.
- W2167322592 countsByYear W21673225922017 @default.
- W2167322592 countsByYear W21673225922018 @default.
- W2167322592 countsByYear W21673225922019 @default.
- W2167322592 countsByYear W21673225922020 @default.
- W2167322592 countsByYear W21673225922021 @default.
- W2167322592 countsByYear W21673225922022 @default.
- W2167322592 countsByYear W21673225922023 @default.
- W2167322592 crossrefType "journal-article" @default.
- W2167322592 hasAuthorship W2167322592A5086256968 @default.
- W2167322592 hasAuthorship W2167322592A5087137800 @default.
- W2167322592 hasBestOaLocation W21673225921 @default.
- W2167322592 hasConcept C104317684 @default.
- W2167322592 hasConcept C143065580 @default.
- W2167322592 hasConcept C2777893358 @default.
- W2167322592 hasConcept C2778952914 @default.
- W2167322592 hasConcept C2779413141 @default.
- W2167322592 hasConcept C55493867 @default.
- W2167322592 hasConcept C60987743 @default.