FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2167440544> ?p ?o ?g. }

• W2167440544 endingPage "2325" @default.
• W2167440544 startingPage "2318" @default.
• W2167440544 abstract "We previously found that the splicing of exon 5 to exon 6 in the rat beta-TM gene required that exon 6 first be joined to the downstream common exon 8 (Helfman et al., Genes and Dev. 2, 1627-1638, 1988). Pre-mRNAs containing exon 5, intron 5 and exon 6 are not normally spliced in vitro. We have carried out a mutational analysis to determine which sequences in the pre-mRNA contribute to the inability of this precursor to be spliced in vitro. We found that mutations in two regions of the pre-mRNA led to activation of the 3'-splice site of exon 6, without first joining exon 6 to exon 8. First, introduction of a nine nucleotide poly U tract upstream of the 3'-splice site of exon 6 results in the splicing of exon 5 to exon 6 with as little as 35 nucleotides of exon 6. Second, introduction of a consensus 5'-splice site in exon 6 led to splicing of exon 5 to exon 6. Thus, three distinct elements can act independently to activate the use of the 3'-splice site of exon 6: (1) the sequences contained within exon 8 when joined to exon 6, (2) a poly U tract in intron 5, and (3) a consensus 5'-splice site in exon 6. Using biochemical assays, we have determined that these sequence elements interact with distinct cellular factors for 3'-splice site utilization. Although HeLa cell nuclear extracts were able to splice all three types of pre-mRNAs mentioned above, a cytoplasmic S100 fraction supplemented with SR proteins was unable to efficiently splice exon 5 to exon 6 using precursors in which exon 6 was joined to exon 8. We also studied how these elements contribute to alternative splice site selection using precursors containing the mutually exclusive, alternatively spliced cassette comprised of exons 5 through 8. Introduction of the poly U tract upstream of exon 6, and changing the 5'-splice site of exon 6 to a consensus sequence, either alone or in combination, facilitated the use of exon 6 in vitro, such that exon 6 was spliced more efficiently to exon 8. These data show that intron sequences upstream of an exon can contribute to the use of the downstream 5'-splice, and that sequences surrounding exon 6 can contribute to tissue-specific alternative splice site selection." @default.
• W2167440544 created "2016-06-24" @default.
• W2167440544 creator A5010824972 @default.
• W2167440544 creator A5039994535 @default.
• W2167440544 creator A5077207309 @default.
• W2167440544 date "1994-01-01" @default.
• W2167440544 modified "2023-10-13" @default.
• W2167440544 title "Alternative splicing of β-tropomyosin pre-mRNA: multiple cis-elements can contribute to the use of the 5′- and 3′-splice sites of the nonmuscle/smooth muscle exon 6" @default.
• W2167440544 cites W11380702 @default.
• W2167440544 cites W1530353793 @default.
• W2167440544 cites W1532050162 @default.
• W2167440544 cites W1567606707 @default.
• W2167440544 cites W1597757171 @default.
• W2167440544 cites W1750913658 @default.
• W2167440544 cites W1767488132 @default.
• W2167440544 cites W1771001171 @default.
• W2167440544 cites W1783342470 @default.
• W2167440544 cites W1813070175 @default.
• W2167440544 cites W1823586796 @default.
• W2167440544 cites W1864764602 @default.
• W2167440544 cites W1865882613 @default.
• W2167440544 cites W1914628750 @default.
• W2167440544 cites W1942623951 @default.
• W2167440544 cites W1968322556 @default.
• W2167440544 cites W1971981203 @default.
• W2167440544 cites W1975580175 @default.
• W2167440544 cites W1979937918 @default.
• W2167440544 cites W1985576809 @default.
• W2167440544 cites W1989109522 @default.
• W2167440544 cites W1991692416 @default.
• W2167440544 cites W1993243592 @default.
• W2167440544 cites W2008932218 @default.
• W2167440544 cites W2013955971 @default.
• W2167440544 cites W2014791263 @default.
• W2167440544 cites W2021054898 @default.
• W2167440544 cites W2023218737 @default.
• W2167440544 cites W2035961932 @default.
• W2167440544 cites W2036369813 @default.
• W2167440544 cites W2036931150 @default.
• W2167440544 cites W2041847911 @default.
• W2167440544 cites W2044147175 @default.
• W2167440544 cites W2044500704 @default.
• W2167440544 cites W2045984686 @default.
• W2167440544 cites W2053220812 @default.
• W2167440544 cites W2054612314 @default.
• W2167440544 cites W2055681276 @default.
• W2167440544 cites W2057479447 @default.
• W2167440544 cites W2065104935 @default.
• W2167440544 cites W2069252364 @default.
• W2167440544 cites W2074427164 @default.
• W2167440544 cites W2082127202 @default.
• W2167440544 cites W2091612946 @default.
• W2167440544 cites W2097531178 @default.
• W2167440544 cites W2103886753 @default.
• W2167440544 cites W2116884417 @default.
• W2167440544 cites W2124947814 @default.
• W2167440544 cites W2132978049 @default.
• W2167440544 cites W2138270253 @default.
• W2167440544 cites W2140409821 @default.
• W2167440544 cites W2145761775 @default.
• W2167440544 cites W2151078655 @default.
• W2167440544 cites W2166724094 @default.
• W2167440544 cites W2170255408 @default.
• W2167440544 cites W4246303891 @default.
• W2167440544 cites W8823967 @default.
• W2167440544 doi "https://doi.org/10.1093/nar/22.12.2318" @default.
• W2167440544 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/523690" @default.
• W2167440544 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8036160" @default.
• W2167440544 hasPublicationYear "1994" @default.
• W2167440544 type Work @default.
• W2167440544 sameAs 2167440544 @default.
• W2167440544 citedByCount "37" @default.
• W2167440544 countsByYear W21674405442014 @default.
• W2167440544 crossrefType "journal-article" @default.
• W2167440544 hasAuthorship W2167440544A5010824972 @default.
• W2167440544 hasAuthorship W2167440544A5039994535 @default.
• W2167440544 hasAuthorship W2167440544A5077207309 @default.
• W2167440544 hasBestOaLocation W21674405442 @default.
• W2167440544 hasConcept C102063271 @default.
• W2167440544 hasConcept C104317684 @default.
• W2167440544 hasConcept C153911025 @default.
• W2167440544 hasConcept C156446351 @default.
• W2167440544 hasConcept C166342232 @default.
• W2167440544 hasConcept C190017056 @default.
• W2167440544 hasConcept C194583182 @default.
• W2167440544 hasConcept C2778776201 @default.
• W2167440544 hasConcept C2780989783 @default.
• W2167440544 hasConcept C36823959 @default.
• W2167440544 hasConcept C39917842 @default.
• W2167440544 hasConcept C42576000 @default.
• W2167440544 hasConcept C54355233 @default.
• W2167440544 hasConcept C54458228 @default.
• W2167440544 hasConcept C62257209 @default.
• W2167440544 hasConcept C67705224 @default.
• W2167440544 hasConcept C72994624 @default.
• W2167440544 hasConcept C86803240 @default.
• W2167440544 hasConcept C94671646 @default.
• W2167440544 hasConceptScore W2167440544C102063271 @default.

• next