FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2167658045> ?p ?o ?g. }

• W2167658045 endingPage "46" @default.
• W2167658045 startingPage "37" @default.
• W2167658045 abstract "Abstract Introduction Glycoproteomics is undergoing rapid development, largely as a result of advances in technologies for isolating glycoproteins and analyzing glycan structures. However, given the number and diversity of glycans, there is need for new technologies that can more rapidly provide differential carbohydrate–protein structural information on a large scale. We describe a new microarray platform based on a label-free imaging ellipsometry technique, which permits simultaneous detection of multiple glycoprotein–lectin interactions without the need for reporter labels, while still providing high throughput kinetic information at much lower cost. Our results demonstrate the utility of LFIRE™ (Label-Free Internal Reflection Ellipsometry) for the rapid kinetic screening of carbohydrate–lectin recognition. The technology was also used to evaluate the benefits of the lectin immobilization format using multi-lectin affinity chromatography (M-LAC) to capture glycoproteins (with enhanced binding strength or avidity) from biological samples. Using a printed panel of lectins, singly or in combination, we examined the binding characteristics of standard glycoproteins. Results and Discussion Using kinetic measurements, it was observed that the binding strength of lectins to carbohydrates is enhanced using a multi-lectin strategy, suggesting that improved selectivity and specificity can lead to increased functional avidity. The data presented confirm that this label-free technology can be used to effectively screen single or combinations of lectins. Furthermore, the combination of LFIRE™ and M-LAC may permit more rapid and sensitive identification of novel biomarkers based on carbohydrate changes in glycoproteins, and lead to a better understanding of the connections of glycan function in cellular mechanisms of health and disease." @default.
• W2167658045 created "2016-06-24" @default.
• W2167658045 creator A5008968439 @default.
• W2167658045 creator A5016776601 @default.
• W2167658045 creator A5023182037 @default.
• W2167658045 creator A5025109446 @default.
• W2167658045 creator A5039606426 @default.
• W2167658045 creator A5047439288 @default.
• W2167658045 creator A5082742285 @default.
• W2167658045 date "2008-06-01" @default.
• W2167658045 modified "2023-10-14" @default.
• W2167658045 title "Kinetic Analysis of Glycoprotein–Lectin Interactions by Label-Free Internal Reflection Ellipsometry" @default.
• W2167658045 cites W1486835939 @default.
• W2167658045 cites W1553087448 @default.
• W2167658045 cites W1771609665 @default.
• W2167658045 cites W1965013033 @default.
• W2167658045 cites W1975318686 @default.
• W2167658045 cites W1982572429 @default.
• W2167658045 cites W1990783969 @default.
• W2167658045 cites W1994014122 @default.
• W2167658045 cites W1995152766 @default.
• W2167658045 cites W1995243908 @default.
• W2167658045 cites W1995404499 @default.
• W2167658045 cites W2001151182 @default.
• W2167658045 cites W2004125187 @default.
• W2167658045 cites W2004391976 @default.
• W2167658045 cites W2006339350 @default.
• W2167658045 cites W2021342788 @default.
• W2167658045 cites W2024017427 @default.
• W2167658045 cites W2028299127 @default.
• W2167658045 cites W2036650483 @default.
• W2167658045 cites W2043714366 @default.
• W2167658045 cites W2044879576 @default.
• W2167658045 cites W2055237590 @default.
• W2167658045 cites W2066278717 @default.
• W2167658045 cites W2071677970 @default.
• W2167658045 cites W2083077313 @default.
• W2167658045 cites W2083260383 @default.
• W2167658045 cites W2085968705 @default.
• W2167658045 cites W2092602198 @default.
• W2167658045 cites W2093986499 @default.
• W2167658045 cites W2116274140 @default.
• W2167658045 cites W2123631149 @default.
• W2167658045 cites W2125811479 @default.
• W2167658045 cites W2152245174 @default.
• W2167658045 cites W2155385276 @default.
• W2167658045 cites W2155963087 @default.
• W2167658045 cites W2168401402 @default.
• W2167658045 cites W2283917576 @default.
• W2167658045 cites W4244641708 @default.
• W2167658045 doi "https://doi.org/10.1007/s12014-008-9007-y" @default.
• W2167658045 hasPublicationYear "2008" @default.
• W2167658045 type Work @default.
• W2167658045 sameAs 2167658045 @default.
• W2167658045 citedByCount "17" @default.
• W2167658045 countsByYear W21676580452012 @default.
• W2167658045 countsByYear W21676580452013 @default.
• W2167658045 countsByYear W21676580452015 @default.
• W2167658045 countsByYear W21676580452016 @default.
• W2167658045 countsByYear W21676580452021 @default.
• W2167658045 crossrefType "journal-article" @default.
• W2167658045 hasAuthorship W2167658045A5008968439 @default.
• W2167658045 hasAuthorship W2167658045A5016776601 @default.
• W2167658045 hasAuthorship W2167658045A5023182037 @default.
• W2167658045 hasAuthorship W2167658045A5025109446 @default.
• W2167658045 hasAuthorship W2167658045A5039606426 @default.
• W2167658045 hasAuthorship W2167658045A5047439288 @default.
• W2167658045 hasAuthorship W2167658045A5082742285 @default.
• W2167658045 hasBestOaLocation W21676580451 @default.
• W2167658045 hasConcept C104317684 @default.
• W2167658045 hasConcept C108625454 @default.
• W2167658045 hasConcept C147483822 @default.
• W2167658045 hasConcept C156534840 @default.
• W2167658045 hasConcept C179223381 @default.
• W2167658045 hasConcept C185592680 @default.
• W2167658045 hasConcept C203014093 @default.
• W2167658045 hasConcept C206212055 @default.
• W2167658045 hasConcept C2775905019 @default.
• W2167658045 hasConcept C2780758401 @default.
• W2167658045 hasConcept C55493867 @default.
• W2167658045 hasConcept C70721500 @default.
• W2167658045 hasConcept C86803240 @default.
• W2167658045 hasConceptScore W2167658045C104317684 @default.
• W2167658045 hasConceptScore W2167658045C108625454 @default.
• W2167658045 hasConceptScore W2167658045C147483822 @default.
• W2167658045 hasConceptScore W2167658045C156534840 @default.
• W2167658045 hasConceptScore W2167658045C179223381 @default.
• W2167658045 hasConceptScore W2167658045C185592680 @default.
• W2167658045 hasConceptScore W2167658045C203014093 @default.
• W2167658045 hasConceptScore W2167658045C206212055 @default.
• W2167658045 hasConceptScore W2167658045C2775905019 @default.
• W2167658045 hasConceptScore W2167658045C2780758401 @default.
• W2167658045 hasConceptScore W2167658045C55493867 @default.
• W2167658045 hasConceptScore W2167658045C70721500 @default.
• W2167658045 hasConceptScore W2167658045C86803240 @default.
• W2167658045 hasIssue "1-2" @default.
• W2167658045 hasLocation W21676580451 @default.
• W2167658045 hasOpenAccess W2167658045 @default.

• next