FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2167658120> ?p ?o ?g. }

• W2167658120 endingPage "88" @default.
• W2167658120 startingPage "75" @default.
• W2167658120 abstract "In the present study, we have investigated the potential regulation of thyroglobulin (Tg) and extracellular matrix components synthesis by thyroid-stimulating hormone (TSH) and tetradecanoyl phorbol-13-acetate (TPA) on thyroid cells. Porcine thyroid cells isolated by trypsin-EGTA digestion of thyroid glands were maintained in serum containing medium on poly (L-lysine)-coated dishes. Cells differentiated into follicular or vesicular-like structures were distinguished by their ability to organify Na[125l] and to respond to TSH stimulation. After an incubation of the cells with radiolabeled proline or methionine, two major proteins were identified, p450–480 and p290 (so named because of their molecular masses). Tg (p290) synthesis was demonstrated by the synthesis of [131l]-labeled polypeptides with electrophoretic properties identical to those of authentic Tg molecules. P450–480 resolved to Mr 190,000 under reducing sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) conditions. It was identified as thrombospondin by its reactivity with a monoclonal anti-human thrombospondin and by peptide sequencing of some of its tryptic fragments that displayed identity to thrombospondin l. Collagen synthesis was demonstrated by the formation of radioactive hydroxyproline and by the synthesis of pepsin-resistant polypeptides ranging from Mrs 120,000 to 200,000. When the cells were cultured in the presence of 100 nM TPA, the culture medium contents of thrombospondin and collagen were increased by 2.7 and 1.6-fold, respectively, whereas Tg content was decreased by a factor 3.9. In contrast, the acute treatment of control cells with TPA induced a decrease in both Tg and collagen content by factors 3.0 and 1.5, respectively, and an increase in thrombospondin content by a factor 2.5. In the presence of 100 nM TPA, TSH (1 mU/ml) did not counteract the stimulating effect of TPA on extracellular matrix components synthesis. In contrast, when cells were cultured in the presence of TSH alone at concentrations higher than 0.1 mU/ml, collagen and thrombospondin in the medium were decreased by a factor 2.0 and 1.9, respectively, and TSH preferentially activated Tg synthesis. However, no acute response to TSH was observed in cells incubated for 2 days without effectors (control cells). On TSH differentiated cells, TPA decreased both collagen and Tg accumulation by factor 1.2 and 1.8, respectively, whereas it increased the one of thrombospondin by a factor 2. These results, together with the stimulating effect of TPA on TSH mediated cell proliferation, argue for a role of thrombospondin in cell adhesion and migration events within the thyroid epithelium. © 1994 Wiley-Liss, Inc." @default.
• W2167658120 created "2016-06-24" @default.
• W2167658120 creator A5003136625 @default.
• W2167658120 creator A5024627583 @default.
• W2167658120 creator A5049635748 @default.
• W2167658120 creator A5053037768 @default.
• W2167658120 creator A5067919860 @default.
• W2167658120 creator A5070275035 @default.
• W2167658120 creator A5081946568 @default.
• W2167658120 date "1994-07-01" @default.
• W2167658120 modified "2023-09-25" @default.
• W2167658120 title "Differential expression of thrombospondin, collagen, and thyroglobulin by thyroid-stimulating hormone and tumor-promoting phorbol ester in cultured porcine thyroid cells" @default.
• W2167658120 cites W1501863981 @default.
• W2167658120 cites W1523515258 @default.
• W2167658120 cites W1538283518 @default.
• W2167658120 cites W1545711672 @default.
• W2167658120 cites W1555964835 @default.
• W2167658120 cites W1564139422 @default.
• W2167658120 cites W1570298666 @default.
• W2167658120 cites W1577670416 @default.
• W2167658120 cites W1593476150 @default.
• W2167658120 cites W1595343629 @default.
• W2167658120 cites W1598116581 @default.
• W2167658120 cites W1605408848 @default.
• W2167658120 cites W1613957108 @default.
• W2167658120 cites W1634254199 @default.
• W2167658120 cites W1636941026 @default.
• W2167658120 cites W1670794068 @default.
• W2167658120 cites W171910026 @default.
• W2167658120 cites W1775749144 @default.
• W2167658120 cites W1866867806 @default.
• W2167658120 cites W1934065093 @default.
• W2167658120 cites W1966940470 @default.
• W2167658120 cites W1968866935 @default.
• W2167658120 cites W1969014509 @default.
• W2167658120 cites W1971865294 @default.
• W2167658120 cites W1976566546 @default.
• W2167658120 cites W1979644658 @default.
• W2167658120 cites W1990129530 @default.
• W2167658120 cites W1999026704 @default.
• W2167658120 cites W2000098606 @default.
• W2167658120 cites W2001578365 @default.
• W2167658120 cites W2009533573 @default.
• W2167658120 cites W2009856702 @default.
• W2167658120 cites W2010755118 @default.
• W2167658120 cites W2015121214 @default.
• W2167658120 cites W2019012651 @default.
• W2167658120 cites W2022097994 @default.
• W2167658120 cites W2025077320 @default.
• W2167658120 cites W2040328047 @default.
• W2167658120 cites W2042743000 @default.
• W2167658120 cites W2042843602 @default.
• W2167658120 cites W2043890456 @default.
• W2167658120 cites W2046223523 @default.
• W2167658120 cites W2049143741 @default.
• W2167658120 cites W2049901140 @default.
• W2167658120 cites W2057625739 @default.
• W2167658120 cites W2058137404 @default.
• W2167658120 cites W2059408025 @default.
• W2167658120 cites W2062510144 @default.
• W2167658120 cites W2069501076 @default.
• W2167658120 cites W2070445992 @default.
• W2167658120 cites W2073517579 @default.
• W2167658120 cites W2074557086 @default.
• W2167658120 cites W2085931182 @default.
• W2167658120 cites W2086027018 @default.
• W2167658120 cites W2086288191 @default.
• W2167658120 cites W2088310462 @default.
• W2167658120 cites W2089345041 @default.
• W2167658120 cites W2089920690 @default.
• W2167658120 cites W2094071205 @default.
• W2167658120 cites W2100837269 @default.
• W2167658120 cites W2105657469 @default.
• W2167658120 cites W2127440469 @default.
• W2167658120 cites W2129886211 @default.
• W2167658120 cites W2150580488 @default.
• W2167658120 cites W2153365102 @default.
• W2167658120 cites W2400443034 @default.
• W2167658120 cites W4239456555 @default.
• W2167658120 doi "https://doi.org/10.1002/jcp.1041600110" @default.
• W2167658120 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8021301" @default.
• W2167658120 hasPublicationYear "1994" @default.
• W2167658120 type Work @default.
• W2167658120 sameAs 2167658120 @default.
• W2167658120 citedByCount "22" @default.
• W2167658120 crossrefType "journal-article" @default.
• W2167658120 hasAuthorship W2167658120A5003136625 @default.
• W2167658120 hasAuthorship W2167658120A5024627583 @default.
• W2167658120 hasAuthorship W2167658120A5049635748 @default.
• W2167658120 hasAuthorship W2167658120A5053037768 @default.
• W2167658120 hasAuthorship W2167658120A5067919860 @default.
• W2167658120 hasAuthorship W2167658120A5070275035 @default.
• W2167658120 hasAuthorship W2167658120A5081946568 @default.
• W2167658120 hasConcept C126322002 @default.
• W2167658120 hasConcept C134018914 @default.
• W2167658120 hasConcept C153911025 @default.
• W2167658120 hasConcept C167814343 @default.
• W2167658120 hasConcept C181199279 @default.

• next