FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2167669991> ?p ?o ?g. }

• W2167669991 endingPage "1049" @default.
• W2167669991 startingPage "1045" @default.
• W2167669991 abstract "ABSTRACT Temperature gradient gel electrophoresis (TGGE) is well suited for fingerprinting bacterial communities by separating PCR-amplified fragments of 16S rRNA genes (16S ribosomal DNA [rDNA]). A strategy was developed and was generally applicable for linking 16S rDNA from community fingerprints to pure culture isolates from the same habitat. For this, digoxigenin-labeled polynucleotide probes were generated by PCR, using bands excised from TGGE community fingerprints as a template, and applied in hybridizations with dot blotted 16S rDNA amplified from bacterial isolates. Within 16S rDNA, the hypervariable V6 region, corresponding to positions 984 to 1047 ( Escherichia coli 16S rDNA sequence), which is a subset of the region used for TGGE (positions 968 to 1401), best met the criteria of high phylogenetic variability, required for sufficient probe specificity, and closely flanking conserved priming sites for amplification. Removal of flanking conserved bases was necessary to enable the differentiation of closely related species. This was achieved by 5′ exonuclease digestion, terminated by phosphorothioate bonds which were synthesized into the primers. The remaining complementary strand was removed by single-strand-specific digestion. Standard hybridization with truncated probes allowed differentiation of bacteria which differed by only two bases within the probe target site and 1.2% within the complete 16S rDNA. However, a truncated probe, derived from an excised TGGE band of a rhizosphere community, hybridized with three phylogenetically related isolates with identical V6 sequences. Only one of the isolates comigrated with the excised band in TGGE, which was shown to be due to identical sequences, demonstrating the utility of a combined TGGE and V6 probe approach." @default.
• W2167669991 created "2016-06-24" @default.
• W2167669991 creator A5040459043 @default.
• W2167669991 creator A5042212209 @default.
• W2167669991 creator A5056432305 @default.
• W2167669991 creator A5073430026 @default.
• W2167669991 creator A5089800878 @default.
• W2167669991 date "1999-03-01" @default.
• W2167669991 modified "2023-09-23" @default.
• W2167669991 title "Polynucleotide Probes That Target a Hypervariable Region of 16S rRNA Genes To Identify Bacterial Isolates Corresponding to Bands of Community Fingerprints" @default.
• W2167669991 cites W1547852780 @default.
• W2167669991 cites W1597698279 @default.
• W2167669991 cites W1598574080 @default.
• W2167669991 cites W1964219271 @default.
• W2167669991 cites W1967184988 @default.
• W2167669991 cites W1992393494 @default.
• W2167669991 cites W2013620980 @default.
• W2167669991 cites W2026476601 @default.
• W2167669991 cites W2034056649 @default.
• W2167669991 cites W2044239065 @default.
• W2167669991 cites W2068687524 @default.
• W2167669991 cites W2069139138 @default.
• W2167669991 cites W2075086069 @default.
• W2167669991 cites W2079446230 @default.
• W2167669991 cites W2089496565 @default.
• W2167669991 cites W2099982193 @default.
• W2167669991 cites W2103170384 @default.
• W2167669991 cites W2114136095 @default.
• W2167669991 cites W2126321303 @default.
• W2167669991 cites W2129867161 @default.
• W2167669991 cites W2131988453 @default.
• W2167669991 cites W2132598047 @default.
• W2167669991 cites W2137090500 @default.
• W2167669991 cites W2156336689 @default.
• W2167669991 cites W2159620478 @default.
• W2167669991 cites W2167180773 @default.
• W2167669991 cites W2170643164 @default.
• W2167669991 cites W2312479496 @default.
• W2167669991 cites W4212864994 @default.
• W2167669991 cites W67115383 @default.
• W2167669991 doi "https://doi.org/10.1128/aem.65.3.1045-1049.1999" @default.
• W2167669991 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/91142" @default.
• W2167669991 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10049861" @default.
• W2167669991 hasPublicationYear "1999" @default.
• W2167669991 type Work @default.
• W2167669991 sameAs 2167669991 @default.
• W2167669991 citedByCount "148" @default.
• W2167669991 countsByYear W21676699912012 @default.
• W2167669991 countsByYear W21676699912013 @default.
• W2167669991 countsByYear W21676699912014 @default.
• W2167669991 countsByYear W21676699912015 @default.
• W2167669991 countsByYear W21676699912016 @default.
• W2167669991 countsByYear W21676699912018 @default.
• W2167669991 countsByYear W21676699912019 @default.
• W2167669991 countsByYear W21676699912020 @default.
• W2167669991 countsByYear W21676699912022 @default.
• W2167669991 countsByYear W21676699912023 @default.
• W2167669991 crossrefType "journal-article" @default.
• W2167669991 hasAuthorship W2167669991A5040459043 @default.
• W2167669991 hasAuthorship W2167669991A5042212209 @default.
• W2167669991 hasAuthorship W2167669991A5056432305 @default.
• W2167669991 hasAuthorship W2167669991A5073430026 @default.
• W2167669991 hasAuthorship W2167669991A5089800878 @default.
• W2167669991 hasBestOaLocation W21676699911 @default.
• W2167669991 hasConcept C104317684 @default.
• W2167669991 hasConcept C134494468 @default.
• W2167669991 hasConcept C145169222 @default.
• W2167669991 hasConcept C153911025 @default.
• W2167669991 hasConcept C178790620 @default.
• W2167669991 hasConcept C185592680 @default.
• W2167669991 hasConcept C193252679 @default.
• W2167669991 hasConcept C207812311 @default.
• W2167669991 hasConcept C2777563447 @default.
• W2167669991 hasConcept C42062724 @default.
• W2167669991 hasConcept C49105822 @default.
• W2167669991 hasConcept C54355233 @default.
• W2167669991 hasConcept C547475151 @default.
• W2167669991 hasConcept C67905577 @default.
• W2167669991 hasConcept C81566733 @default.
• W2167669991 hasConcept C86803240 @default.
• W2167669991 hasConceptScore W2167669991C104317684 @default.
• W2167669991 hasConceptScore W2167669991C134494468 @default.
• W2167669991 hasConceptScore W2167669991C145169222 @default.
• W2167669991 hasConceptScore W2167669991C153911025 @default.
• W2167669991 hasConceptScore W2167669991C178790620 @default.
• W2167669991 hasConceptScore W2167669991C185592680 @default.
• W2167669991 hasConceptScore W2167669991C193252679 @default.
• W2167669991 hasConceptScore W2167669991C207812311 @default.
• W2167669991 hasConceptScore W2167669991C2777563447 @default.
• W2167669991 hasConceptScore W2167669991C42062724 @default.
• W2167669991 hasConceptScore W2167669991C49105822 @default.
• W2167669991 hasConceptScore W2167669991C54355233 @default.
• W2167669991 hasConceptScore W2167669991C547475151 @default.
• W2167669991 hasConceptScore W2167669991C67905577 @default.
• W2167669991 hasConceptScore W2167669991C81566733 @default.
• W2167669991 hasConceptScore W2167669991C86803240 @default.
• W2167669991 hasIssue "3" @default.
• W2167669991 hasLocation W21676699911 @default.

• next