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• W2167715010 abstract "The divalent abundant cation Magnesium (Mg2+) has been known to play an important regulatory role in the stimulus-secretion coupling events in a number of epithelial secretory cells including the exocrine pancreas, lachrymal and the parietal cells. Since the salivary glands acinar cells have a similar structure and function as the exocrine pancreas, the stomach and the lachrymal gland, it was decided to investigate specifically the effect of perturbation of extracellular magnesium ([Mg2+]0) on both basal and secretagogue-evoked amylase secretion and total protein output and intracellular free calcium concentrations ([Ca2+]i) in the rat submandibular and parotid glands using spectrofluorimetry and spectroscopic techniques. The results have shown that both zero and elevated (5 and 10 mM) [Mg2+]0 can significantly (P<0.01) inhibit basal amylase or protein output compared to normal (1.1 mM) [Mg2+]0 Either acetylcholine (ACh), noradrenaline (NA) or Phenylephrine (Phe) can elicit marked increases in amylase secretion and total protein output from the isolated parotid and submandibular gland segments respectively in normal [Mg2+]0. In contrast, in the presence of either zero or elevated (5 and 10 mM) [Mg2+]0 the ACh, NA and Phe-evoked amylase secretion and total protein output were markedly attenuated when compared to normal (1.1 mM) Mg2+]0. The inhibitory effect of zero [Mg2+]0 was much more pronounced when compared to 10 mM [Mg2+]0. A perturbation of [Mg2+]0 had little or no effect on the Electrical Field Stimulation (EFS) -evoked amylase responses in the parotid gland. Isoprenaline-evoked amylase secretion revealed a different pattern of secretion in the presence of [Mg2+]0 perturbation. In Fura-2 loaded salivary acinar cells a perturbation of [Mg2+]0 had no significant effect on basal [Ca2+]i in the parotid gland but elevated (5 and 10 mM) [Mg2+]0 attenuated [Ca2+]i in acinar cells taken from submandibular gland. In acinar cells from both glands ACh (105 M), evoked a marked increase in [Ca2+]i above basal level in [Mg2+]0. The response comprises of an initial rise (peak response) followed by a plateau phase (plateau response) before declining back and stabilising a little above control level. In the presence of either zero or elevated (5 and 10 mM) [Mg2+]0 the ACh evoked increases (both peak and plateau phases) in [Ca2+]i was significantly (P<0.05) attenuated compared to normal (1.1 mM) [Mg2+]0. In a nominally free Ca2+ medium containing 1mM Ethyl Glycol Tetracetic Acid (EGTA), ACh evoked only the initial Ca2+ peak, which was sensitive to Mg2+ perturbation. Reperfusion of acinar cells with normal Ca2+ medium resulted in marked increases in [Ca2+]i, which was also sensitive to perturbation of [Mg2+]0. These results indicate that Mg2+ is regulating cellular Ca2+ homeostasis. In Magfura 2-loaded parotid acinar cells an increasing perturbation of [Mg2+]0 resulted in a gradual increase in intracellular free Mg2+ concentrations ([Mg2+])i. Stimulation of the cells with ACh resulted in a significant (P<0.01) decrease in [Mg2+]i. Treatment of Magftira-2 loaded parotid acinar cells with either zero extracellular sodium ([Na+]0) (substituting it with N-Methyl-D-Glucamine (NMDG)), dinitrophenol (DNP) bumetanide, amiloride, quinidine or lidocaine resulted in significant (P<0.01) increases in [Mg2+]i. In the presence of these inhibitors ACh still stimulated a reduction in {Mg2+]i. Taken together, these studies on animal model have demonstrated marked interactions between Ca2+ and Mg2+ signalling during the stimulus-secretion coupling process in the salivary glands. The study also employs male and female human subjects to investigate the rate of salivary secretion and the quality of saliva during normal healthy and such pathophysiolgical conditions such as ageing, diabetes and surgical procedures. The results have demonstrated that either the ageing process or type I and type II diabetes are associated with significant (P<0.01) decreases in salivary secretion. However, both ageing and type I (but not type II diabetes) are associated with increased levels of protein in the saliva. Both ageing and diabetes are associated with significant (P<0.05) increases in Ca2+ levels and significant decreases in Mg2+ and Zn2+ levels in saliva compared to age-matched controls. Ageing and diabetes are also associated with reduced levels of K in the saliva. Although type II diabetic patients presented the same type of salivary changes these were less intense when compared to type I diabetic. Surgical procedures had little or no effect on the various salivary parameters measured except for small increase in resting salivary output and protein concentration. These results indicate that both ageing and diabetes can affect markedly the function of the salivary glands leading to decreased secretion in saliva and to a variation in its composition." @default.
• W2167715010 created "2016-06-24" @default.
• W2167715010 creator A5002582790 @default.
• W2167715010 date "2003-08-01" @default.
• W2167715010 modified "2023-09-27" @default.
• W2167715010 title "Influence of magnesium on salivary gland secretion : physiological and pathophysiological studies" @default.
• W2167715010 cites W1245295209 @default.
• W2167715010 cites W145367462 @default.
• W2167715010 cites W145781537 @default.
• W2167715010 cites W145796029 @default.
• W2167715010 cites W1501383613 @default.
• W2167715010 cites W1537877734 @default.
• W2167715010 cites W1554612460 @default.
• W2167715010 cites W1556314487 @default.
• W2167715010 cites W1558821416 @default.
• W2167715010 cites W1559870773 @default.
• W2167715010 cites W1563063105 @default.
• W2167715010 cites W1570342789 @default.
• W2167715010 cites W1574860654 @default.
• W2167715010 cites W1604520481 @default.
• W2167715010 cites W1617855752 @default.
• W2167715010 cites W168193132 @default.
• W2167715010 cites W1682939406 @default.
• W2167715010 cites W1775749144 @default.
• W2167715010 cites W1781104219 @default.
• W2167715010 cites W1784960910 @default.
• W2167715010 cites W1810878070 @default.
• W2167715010 cites W1820847166 @default.
• W2167715010 cites W1871370962 @default.
• W2167715010 cites W1872309191 @default.
• W2167715010 cites W1909193689 @default.
• W2167715010 cites W1912931109 @default.
• W2167715010 cites W1944669693 @default.
• W2167715010 cites W1956451604 @default.
• W2167715010 cites W1966514705 @default.
• W2167715010 cites W1967457822 @default.
• W2167715010 cites W1972799168 @default.
• W2167715010 cites W1974208320 @default.
• W2167715010 cites W1974790027 @default.
• W2167715010 cites W1975315668 @default.
• W2167715010 cites W1980081860 @default.
• W2167715010 cites W1980166477 @default.
• W2167715010 cites W1980579387 @default.
• W2167715010 cites W1980747493 @default.
• W2167715010 cites W1983898400 @default.
• W2167715010 cites W1983985175 @default.
• W2167715010 cites W1984212762 @default.
• W2167715010 cites W1986315461 @default.
• W2167715010 cites W1990289617 @default.
• W2167715010 cites W1990972018 @default.
• W2167715010 cites W1991326471 @default.
• W2167715010 cites W1991387928 @default.
• W2167715010 cites W1992370195 @default.
• W2167715010 cites W1993282564 @default.
• W2167715010 cites W1993716283 @default.
• W2167715010 cites W1995644134 @default.
• W2167715010 cites W1997058078 @default.
• W2167715010 cites W1997778815 @default.
• W2167715010 cites W1998606603 @default.
• W2167715010 cites W2000135686 @default.
• W2167715010 cites W2001211531 @default.
• W2167715010 cites W2004065791 @default.
• W2167715010 cites W2004349553 @default.
• W2167715010 cites W2006850107 @default.
• W2167715010 cites W2007931463 @default.
• W2167715010 cites W2008095023 @default.
• W2167715010 cites W2008245136 @default.
• W2167715010 cites W2011152653 @default.
• W2167715010 cites W2011516382 @default.
• W2167715010 cites W2011946117 @default.
• W2167715010 cites W2012246710 @default.
• W2167715010 cites W2019783997 @default.
• W2167715010 cites W2022597663 @default.
• W2167715010 cites W2025612821 @default.
• W2167715010 cites W2026537771 @default.
• W2167715010 cites W2026750437 @default.
• W2167715010 cites W2027822231 @default.
• W2167715010 cites W2029822897 @default.
• W2167715010 cites W2032508216 @default.
• W2167715010 cites W2038162808 @default.
• W2167715010 cites W2040110400 @default.
• W2167715010 cites W2041146677 @default.
• W2167715010 cites W2041690080 @default.
• W2167715010 cites W2042111893 @default.
• W2167715010 cites W2042358844 @default.
• W2167715010 cites W2042925862 @default.
• W2167715010 cites W2043963543 @default.
• W2167715010 cites W2047820042 @default.
• W2167715010 cites W2048218470 @default.
• W2167715010 cites W2048482656 @default.
• W2167715010 cites W2054537407 @default.
• W2167715010 cites W2055171082 @default.
• W2167715010 cites W2057530697 @default.
• W2167715010 cites W2057665893 @default.
• W2167715010 cites W2058006543 @default.
• W2167715010 cites W2058450023 @default.
• W2167715010 cites W2059884610 @default.
• W2167715010 cites W2063289493 @default.
• W2167715010 cites W2067193095 @default.
• W2167715010 cites W2068877244 @default.

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