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- W2167746005 abstract "Abstract 4‐Coumarate:CoA ligase (4CL, EC 6.2.1.12) in the phenylpropanoid pathway in plants has attracted interest as a novel target for developing effective plant growth inhibitors (PGIs). In a previous study in which the 4CL inhibitory activity of 28 existing herbicides was investigated using an optimized in vitro screening assay, 4CL activity was found to be strongly inhibited by propanil and swep at 100 µ M . Here, further experimental evidence is provided to substantiate the previous result. Using 4‐coumaric acid as substrate, tobacco 4CL activity was inhibited by propanil or swep in a concentration‐dependent manner, with 50% inhibition concentrations (I 50 ) of 39.6 and 6 µ M respectively. These herbicides also exhibited uncompetitive inhibition towards 4‐coumaric acid. Furthermore, 4CLs from several plant species were inhibited by the herbicides within a range from 1 to 50 µ M . It is proposed that these herbicides have another site of action as a result of the inhibition of 4CL in the phenylpropanoid pathway, and this enzyme represents a new target site for the development of PGI. Copyright © 2007 Society of Chemical Industry" @default.
- W2167746005 created "2016-06-24" @default.
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- W2167746005 date "2007-06-13" @default.
- W2167746005 modified "2023-10-15" @default.
- W2167746005 title "Propanil and swep inhibit 4-coumarate:CoA ligase activityin vitro" @default.
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- W2167746005 doi "https://doi.org/10.1002/ps.1404" @default.
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