FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2167798145> ?p ?o ?g. }

• W2167798145 endingPage "1225" @default.
• W2167798145 startingPage "1218" @default.
• W2167798145 abstract "ABSTRACT Freezing and lyophilization are common methods used for preservation and storage of microorganisms during the production of concentrated starter cultures destined for industrial fermentations or product formulations. The compatible solute trehalose has been widely reported to protect bacterial, yeast and animal cells against a variety of environmental stresses, particularly freezing and dehydration. Analysis of the Lactobacillus acidophilus NCFM genome revealed a putative trehalose utilization locus consisting of a transcriptional regulator, treR ; a trehalose phosphoenolpyruvate transferase system (PTS) transporter, treB ; and a trehalose-6-phosphate hydrolase, treC . The objective of this study was to characterize the tre locus in L. acidophilus and determine whether or not intracellular uptake of trehalose contributes to cryoprotection. Cells subjected to repeated freezing and thawing cycles were monitored for survival in the presence of various concentrations of trehalose. At 20% trehalose a 2-log increase in survival was observed. The trehalose PTS transporter and trehalose hydrolase were disrupted by targeted plasmid insertions. The resulting mutants were unable to grow on trehalose, indicating that both trehalose transport into the cell via a PTS and hydrolysis via a trehalose-6-phosphate hydrolase were necessary for trehalose fermentation. Trehalose uptake was found to be significantly reduced in the transporter mutant but unaffected in the hydrolase mutant. Additionally, the cryoprotective effect of trehalose was reduced in these mutants, suggesting that intracellular transport and hydrolysis contribute significantly to cryoprotection." @default.
• W2167798145 created "2016-06-24" @default.
• W2167798145 creator A5021105547 @default.
• W2167798145 creator A5023192192 @default.
• W2167798145 creator A5032251844 @default.
• W2167798145 creator A5085166852 @default.
• W2167798145 date "2006-02-01" @default.
• W2167798145 modified "2023-10-05" @default.
• W2167798145 title "Characterization of the <i>tre</i> Locus and Analysis of Trehalose Cryoprotection in <i>Lactobacillus acidophilus</i> NCFM" @default.
• W2167798145 cites W1568417131 @default.
• W2167798145 cites W1607746384 @default.
• W2167798145 cites W1652704082 @default.
• W2167798145 cites W1728723996 @default.
• W2167798145 cites W1816602264 @default.
• W2167798145 cites W1970874044 @default.
• W2167798145 cites W1978529058 @default.
• W2167798145 cites W1983233459 @default.
• W2167798145 cites W1986135828 @default.
• W2167798145 cites W1997370001 @default.
• W2167798145 cites W1998610434 @default.
• W2167798145 cites W2019941436 @default.
• W2167798145 cites W2020306391 @default.
• W2167798145 cites W2026158782 @default.
• W2167798145 cites W2029570927 @default.
• W2167798145 cites W2034601572 @default.
• W2167798145 cites W2034911276 @default.
• W2167798145 cites W2043860814 @default.
• W2167798145 cites W2045754986 @default.
• W2167798145 cites W2081585058 @default.
• W2167798145 cites W2085215593 @default.
• W2167798145 cites W2092041008 @default.
• W2167798145 cites W2097250650 @default.
• W2167798145 cites W2098298535 @default.
• W2167798145 cites W2102672175 @default.
• W2167798145 cites W2108605550 @default.
• W2167798145 cites W2110720514 @default.
• W2167798145 cites W2113151858 @default.
• W2167798145 cites W2116560054 @default.
• W2167798145 cites W2117960293 @default.
• W2167798145 cites W2120629404 @default.
• W2167798145 cites W2123114512 @default.
• W2167798145 cites W2127739924 @default.
• W2167798145 cites W2148913876 @default.
• W2167798145 cites W2149267014 @default.
• W2167798145 cites W2150268018 @default.
• W2167798145 cites W2161266291 @default.
• W2167798145 cites W4239629847 @default.
• W2167798145 cites W2034153976 @default.
• W2167798145 doi "https://doi.org/10.1128/aem.72.2.1218-1225.2006" @default.
• W2167798145 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1392980" @default.
• W2167798145 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16461669" @default.
• W2167798145 hasPublicationYear "2006" @default.
• W2167798145 type Work @default.
• W2167798145 sameAs 2167798145 @default.
• W2167798145 citedByCount "65" @default.
• W2167798145 countsByYear W21677981452012 @default.
• W2167798145 countsByYear W21677981452013 @default.
• W2167798145 countsByYear W21677981452014 @default.
• W2167798145 countsByYear W21677981452015 @default.
• W2167798145 countsByYear W21677981452016 @default.
• W2167798145 countsByYear W21677981452017 @default.
• W2167798145 countsByYear W21677981452018 @default.
• W2167798145 countsByYear W21677981452019 @default.
• W2167798145 countsByYear W21677981452020 @default.
• W2167798145 countsByYear W21677981452021 @default.
• W2167798145 countsByYear W21677981452022 @default.
• W2167798145 countsByYear W21677981452023 @default.
• W2167798145 crossrefType "journal-article" @default.
• W2167798145 hasAuthorship W2167798145A5021105547 @default.
• W2167798145 hasAuthorship W2167798145A5023192192 @default.
• W2167798145 hasAuthorship W2167798145A5032251844 @default.
• W2167798145 hasAuthorship W2167798145A5085166852 @default.
• W2167798145 hasBestOaLocation W21677981451 @default.
• W2167798145 hasConcept C100544194 @default.
• W2167798145 hasConcept C181199279 @default.
• W2167798145 hasConcept C185592680 @default.
• W2167798145 hasConcept C2777221637 @default.
• W2167798145 hasConcept C2778452849 @default.
• W2167798145 hasConcept C2779222958 @default.
• W2167798145 hasConcept C2780120296 @default.
• W2167798145 hasConcept C2780255968 @default.
• W2167798145 hasConcept C523546767 @default.
• W2167798145 hasConcept C54355233 @default.
• W2167798145 hasConcept C55493867 @default.
• W2167798145 hasConcept C86803240 @default.
• W2167798145 hasConceptScore W2167798145C100544194 @default.
• W2167798145 hasConceptScore W2167798145C181199279 @default.
• W2167798145 hasConceptScore W2167798145C185592680 @default.
• W2167798145 hasConceptScore W2167798145C2777221637 @default.
• W2167798145 hasConceptScore W2167798145C2778452849 @default.
• W2167798145 hasConceptScore W2167798145C2779222958 @default.
• W2167798145 hasConceptScore W2167798145C2780120296 @default.
• W2167798145 hasConceptScore W2167798145C2780255968 @default.
• W2167798145 hasConceptScore W2167798145C523546767 @default.
• W2167798145 hasConceptScore W2167798145C54355233 @default.
• W2167798145 hasConceptScore W2167798145C55493867 @default.
• W2167798145 hasConceptScore W2167798145C86803240 @default.
• W2167798145 hasIssue "2" @default.

• next