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• W2167906757 endingPage "5186" @default.
• W2167906757 startingPage "5182" @default.
• W2167906757 abstract "The origin of phage phi X174 progeny replicative form (RF) DNA synthesis has been inserted into the plasmid vector pBR322 and cloned. In direct contrast to pBR322, the recombinant superhelical plasmids can substitute for phi X174 RFI DNA as template in phi X174-specific reactions in vitro. We have shown that the recombinant plasmids: (i) are cleaved by the phi X174 A protein; (ii) support net synthesis of unit-length single-stranded circular DNA in the presence of the phi X174 A protein and Escherichia coli rep protein, DNA-binding protein, and DNA polymerase III elongation system; (iii) support replication of duplexes catalyzed by the phi X174 A protein and extracts of E. coli." @default.
• W2167906757 created "2016-06-24" @default.
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• W2167906757 creator A5030497198 @default.
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• W2167906757 date "1980-09-01" @default.
• W2167906757 modified "2023-09-24" @default.
• W2167906757 title "In vitro DNA replication of recombinant plasmid DNAs containing the origin of progeny replicative form DNA synthesis of phage phi X174." @default.
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• W2167906757 doi "https://doi.org/10.1073/pnas.77.9.5182" @default.
• W2167906757 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/350021" @default.
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