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• W2168049079 abstract "LETTERS TO THE EDITORLetter to the editor: The use of extracellular, ion-selective microelectrodes to study the function of heterologously expressed transporters in Xenopus oocytesMark D. Parker, Raif Musa-Aziz, and Walter F. BoronMark D. Parker, Raif Musa-Aziz, and Walter F. BoronPublished Online:01 May 2009https://doi.org/10.1152/ajpcell.00044.2009MoreSectionsPDF (31 KB)Download PDF ToolsExport citationAdd to favoritesGet permissionsTrack citations ShareShare onFacebookTwitterLinkedInEmailWeChat to the editor: In a recent issue of American Journal of Physiology-Cell Physiology, Blanchard et al. (1) published an elegant article in which they report that they have “developed the ion-trap technique” that “uses a large ISE [ion-selective electrode] … gently pushed against the surface of an oocyte … [to isolate] a small volume of extracellular solution (the trap) in which transport-dependent changes in ionic concentrations can be detected.”The authors cite others' use of extracellular ISEs, including work from our own laboratory published by Endeward et al. (4), in which we used a large-tipped H+-selective electrode to monitor the depletion of H+ on the surface of a Xenopus oocyte during the influx of CO2 through aquaporin 1, due to the reaction HCO3− + H+ → CO2 + H2O. Blanchard et al. (1) might also have noted the work of Parker et al. (5), in which we used a large-tipped Cl−-selective electrode to measure transporter-mediated efflux of Cl− from oocytes. In both sets of experiments from our laboratory, Dr. Musa-Aziz positioned a 5–15 μM ISE tip at the oocyte surface and then advanced it ∼40–60 μm further to create a dimple. She subsequently monitored the appearance of ions in (or disappearance from) the unstirred layer trapped between the oocyte surface and the abutting electrode tip. This approach is essentially the same as that first described by de Hemptinne and Huguenin (3) in 1984 to measure pH transients (on the surface of muscle) caused by CO2/HCO3− fluxes and then implemented somewhat differently by Chesler (2) in 1986 to monitor pH transients (in the restricted extracellular space near neurons) caused by NH3/NH4+ and CO2/HCO3− fluxes.Blanchard et al. (1) have coined the phrase “ion-trap” and are unique in applying this technology to study transporters under voltage clamp. They attempted to distinguish their work from ours by stating that Endeward et al. (4) used a “simple static [i.e., immobile] extracellular ISE,” which is, in fact, the approach used by all of the aforementioned groups. To our knowledge, the “ion-trap” technique itself was first developed by de Hemptinne and Huguenin (3) and first exploited to study the molecular function of heterologously expressed transporters by our laboratory (4, 5).REFERENCES1 Blanchard MG, Longpre JP, Wallendorff B, Lapointe JY. Measuring ion transport activities in Xenopus oocytes using the ion-trap technique. Am J Physiol Cell Physiol 295: C1464–C1472, 2008.Link | ISI | Google Scholar2 Chesler M. Regulation of intracellular pH in reticulospinal neurones of the lamprey, Petromyzon marinus. J Physiol (Lond) 381: 241–261, 1986.Crossref | Google Scholar3 De Hemptinne A, Huguenin F. The influence of muscle respiration and glycolysis on surface and intracellular pH in fibres of the rat soleus. J Physiol 347: 581–592, 1984.Crossref | PubMed | ISI | Google Scholar4 Endeward V, Musa-Aziz R, Cooper GJ, Chen LM, Pelletier MF, Virkki LV, Supuran CT, King LS, Boron WF, Gros G. Evidence that aquaporin 1 is a major pathway for CO2 transport across the human erythrocyte membrane. FASEB J 20: 1974–1981, 2006.Crossref | PubMed | ISI | Google Scholar5 Parker MD, Musa-Aziz R, Rojas JD, Choi I, Daly CM, Boron WF. Characterization of human SLC4A10 as an electroneutral Na/HCO3 cotransporter (NBCn2) with Cl− self-exchange activity. J Biol Chem 283: 12777–12788, 2008.Crossref | PubMed | ISI | Google ScholarAUTHOR NOTESAddress for reprint requests and other correspondence: M. D. Parker, Dept. of Physiology and Biophysics, Case Western Reserve Univ., Cleveland, OH 44106 (e-mail: [email protected]) Download PDF Previous Back to Top Next FiguresReferencesRelatedInformationCited ByMonitoring Ion Activities In and Around Cells Using Ion-Selective Liquid-Membrane Microelectrodes15 January 2013 | Sensors, Vol. 13, No. 1Reply to “Letter to the editor: ‘The use of extracellular, ion-selective microelectrodes to study the function of heterologously expressed transporters in Xenopus oocytes’”Maxime Blanchard, Jean-Philippe Longpré, and Jean-Yves Lapointe1 May 2009 | American Journal of Physiology-Cell Physiology, Vol. 296, No. 5 More from this issue > Volume 296Issue 5May 2009Pages C1243-C1243 Copyright & PermissionsCopyright © 2009 the American Physiological Societyhttps://doi.org/10.1152/ajpcell.00044.2009PubMed19411590History Published online 1 May 2009 Published in print 1 May 2009 Metrics" @default.
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