FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2168049252> ?p ?o ?g. }

• W2168049252 endingPage "4135" @default.
• W2168049252 startingPage "4129" @default.
• W2168049252 abstract "Chronic wasting disease (CWD) and sheep scrapie can be transmitted via indirect environmental routes, and it is known that soil can serve as a reservoir of prion infectivity. Given the strong interaction between the prion protein (PrP) and soil, we hypothesized that binding to soil enhances prion resistance to enzymatic digestion, thereby facilitating prion longevity in the environment and providing protection from host degradation. We characterized the performance of a commercially available subtilisin enzyme, Prionzyme, to degrade soil-bound and unbound CWD and HY TME PrP as a function of pH, temperature, and treatment time. The subtilisin enzyme effectively degraded PrP adsorbed to a wide range of soils and soil minerals below the limits of detection. Signal loss occurred rapidly at high pH (12.5) and within 7 days under conditions representative of the natural environment (pH 7.4, 22 °C). We observed no apparent difference in enzyme effectiveness between bound and unbound CWD PrP. Our results show that although adsorbed prions do retain relative resistance to enzymatic digestion compared with other brain homogenate proteins, they can be effectively degraded when bound to soil. Our results also suggest a topical application of a subtilisin enzyme solution may be an effective decontamination method to limit disease transmission via environmental “hot spots” of prion infectivity." @default.
• W2168049252 created "2016-06-24" @default.
• W2168049252 creator A5058589583 @default.
• W2168049252 creator A5072948807 @default.
• W2168049252 creator A5080657235 @default.
• W2168049252 creator A5086302568 @default.
• W2168049252 date "2010-05-07" @default.
• W2168049252 modified "2023-09-25" @default.
• W2168049252 title "Enzymatic Digestion of Chronic Wasting Disease Prions Bound to Soil" @default.
• W2168049252 cites W1938969372 @default.
• W2168049252 cites W1969671971 @default.
• W2168049252 cites W1970898725 @default.
• W2168049252 cites W1972085366 @default.
• W2168049252 cites W1975293129 @default.
• W2168049252 cites W1993391917 @default.
• W2168049252 cites W1997935103 @default.
• W2168049252 cites W2004286784 @default.
• W2168049252 cites W2014142698 @default.
• W2168049252 cites W2018790021 @default.
• W2168049252 cites W2020508121 @default.
• W2168049252 cites W2021544273 @default.
• W2168049252 cites W2024632347 @default.
• W2168049252 cites W2026261607 @default.
• W2168049252 cites W2035703126 @default.
• W2168049252 cites W2050488309 @default.
• W2168049252 cites W2065115078 @default.
• W2168049252 cites W2067540111 @default.
• W2168049252 cites W2072758941 @default.
• W2168049252 cites W2078977214 @default.
• W2168049252 cites W2079843273 @default.
• W2168049252 cites W2080811931 @default.
• W2168049252 cites W2087873094 @default.
• W2168049252 cites W2119481622 @default.
• W2168049252 cites W2124577407 @default.
• W2168049252 cites W2129241235 @default.
• W2168049252 cites W2154622161 @default.
• W2168049252 cites W44129923 @default.
• W2168049252 doi "https://doi.org/10.1021/es903520d" @default.
• W2168049252 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2885836" @default.
• W2168049252 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20450190" @default.
• W2168049252 hasPublicationYear "2010" @default.
• W2168049252 type Work @default.
• W2168049252 sameAs 2168049252 @default.
• W2168049252 citedByCount "33" @default.
• W2168049252 countsByYear W21680492522012 @default.
• W2168049252 countsByYear W21680492522013 @default.
• W2168049252 countsByYear W21680492522014 @default.
• W2168049252 countsByYear W21680492522015 @default.
• W2168049252 countsByYear W21680492522017 @default.
• W2168049252 countsByYear W21680492522018 @default.
• W2168049252 countsByYear W21680492522022 @default.
• W2168049252 countsByYear W21680492522023 @default.
• W2168049252 crossrefType "journal-article" @default.
• W2168049252 hasAuthorship W2168049252A5058589583 @default.
• W2168049252 hasAuthorship W2168049252A5072948807 @default.
• W2168049252 hasAuthorship W2168049252A5080657235 @default.
• W2168049252 hasAuthorship W2168049252A5086302568 @default.
• W2168049252 hasBestOaLocation W21680492522 @default.
• W2168049252 hasConcept C106358424 @default.
• W2168049252 hasConcept C142724271 @default.
• W2168049252 hasConcept C159047783 @default.
• W2168049252 hasConcept C181199279 @default.
• W2168049252 hasConcept C185592680 @default.
• W2168049252 hasConcept C2522874641 @default.
• W2168049252 hasConcept C2776460866 @default.
• W2168049252 hasConcept C2776735649 @default.
• W2168049252 hasConcept C2779134260 @default.
• W2168049252 hasConcept C2779728475 @default.
• W2168049252 hasConcept C2779811187 @default.
• W2168049252 hasConcept C2781357212 @default.
• W2168049252 hasConcept C3019804061 @default.
• W2168049252 hasConcept C43617362 @default.
• W2168049252 hasConcept C55493867 @default.
• W2168049252 hasConcept C71924100 @default.
• W2168049252 hasConcept C86803240 @default.
• W2168049252 hasConcept C89423630 @default.
• W2168049252 hasConceptScore W2168049252C106358424 @default.
• W2168049252 hasConceptScore W2168049252C142724271 @default.
• W2168049252 hasConceptScore W2168049252C159047783 @default.
• W2168049252 hasConceptScore W2168049252C181199279 @default.
• W2168049252 hasConceptScore W2168049252C185592680 @default.
• W2168049252 hasConceptScore W2168049252C2522874641 @default.
• W2168049252 hasConceptScore W2168049252C2776460866 @default.
• W2168049252 hasConceptScore W2168049252C2776735649 @default.
• W2168049252 hasConceptScore W2168049252C2779134260 @default.
• W2168049252 hasConceptScore W2168049252C2779728475 @default.
• W2168049252 hasConceptScore W2168049252C2779811187 @default.
• W2168049252 hasConceptScore W2168049252C2781357212 @default.
• W2168049252 hasConceptScore W2168049252C3019804061 @default.
• W2168049252 hasConceptScore W2168049252C43617362 @default.
• W2168049252 hasConceptScore W2168049252C55493867 @default.
• W2168049252 hasConceptScore W2168049252C71924100 @default.
• W2168049252 hasConceptScore W2168049252C86803240 @default.
• W2168049252 hasConceptScore W2168049252C89423630 @default.
• W2168049252 hasIssue "11" @default.
• W2168049252 hasLocation W21680492521 @default.
• W2168049252 hasLocation W21680492522 @default.
• W2168049252 hasLocation W21680492523 @default.

• next