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• W2168106093 abstract "BackgroundLeishmania (Viannia) species are the principal cause of mucosal leishmaniasis. The natural history and pathogenesis of mucosal disease are enigmatic. Parasitological evaluation of mucosal tissues has been constrained by the invasiveness of conventional sampling methods MethodsWe evaluated the presence ofLeishmania in the mucosa of 26 patients with cutaneous leishmaniasis and 2 patients with mucocutaneous leishmaniasis. Swab samples of the nasal mucosa, tonsils, and conjunctiva were analyzed using polymerase chain reaction with LV-B1 primers and Southern blot hybridization ResultsTwo patients with mucocutaneous leishmaniasis and 21 (81%) of 26 patients with cutaneous leishmaniasis had Leishmania kinetoplast minicircle DNA (kDNA) in mucosal tissues. kDNA was amplified from swab samples of nasal mucosa from 14 (58%) of 24 patients, tonsils from 13 (46%) of 28 patients, and conjunctiva from 6 (25%) of 24 patients. kDNA was detected in the mucosa of patients with cutaneous disease caused by Leishmania panamensis, Leishmania guyanensis and Leishmania braziliensis ConclusionThe asymptomatic presence of parasites in mucosal tissues may be common in patients with Leishmania (Viannia) infection" @default.
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• W2168106093 date "2009-08-15" @default.
• W2168106093 modified "2023-09-27" @default.
• W2168106093 title "Detection of<i>Leishmania</i>in Unaffected Mucosal Tissues of Patients with Cutaneous Leishmaniasis Caused by<i>Leishmania (Viannia)</i>Species" @default.
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• W2168106093 doi "https://doi.org/10.1086/600109" @default.
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