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• W2168121009 abstract "Abstract Human visual system is exposed to high levels of natural and artificial lights of different spectra and intensities along lifetime. Light‐emitting diodes ( LED s) are the basic lighting components in screens of PC s, phones and TV sets; hence it is so important to know the implications of LED radiations on the human visual system. The aim of this study was to investigate the effect of LED s radiations on human retinal pigment epithelial cells ( HRPE piC). They were exposed to three light–darkness (12 h/12 h) cycles, using blue‐468 nm, green‐525 nm, red‐616 nm and white light. Cellular viability of HRPE piC was evaluated by labeling all nuclei with DAPI ; Production of reactive oxygen species ( ROS ) was determined by H2 DCFDA staining; mitochondrial membrane potential was quantified by TMRM staining; DNA damage was determined by H2 AX histone activation, and apoptosis was evaluated by caspases‐3,‐7 activation. It is shown that LED radiations decrease 75–99% cellular viability, and increase 66–89% cellular apoptosis. They also increase ROS production and DNA damage. Fluorescence intensity of apoptosis was 3.7% in nonirradiated cells and 88.8%, 86.1%, 83.9% and 65.5% in cells exposed to white, blue, green or red light, respectively. This study indicates three light–darkness (12 h/12 h) cycles of exposure to LED lighting affect in vitro HRPE piC." @default.
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• W2168121009 date "2012-10-09" @default.
• W2168121009 modified "2023-10-16" @default.
• W2168121009 title "Effects of Light-emitting Diode Radiations on Human Retinal Pigment Epithelial Cells<i>In Vitro</i>" @default.
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• W2168121009 doi "https://doi.org/10.1111/j.1751-1097.2012.01237.x" @default.
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