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• W2168183931 abstract "The monoclonal antibodies to porin, an outer membrane protein isolated from Salmonella typhimurium and sandwich enzyme linked immunosorbent assay (ELISA) has made possible the detection of porin from sera of S. typhimurium-infected mice. The specificity of the monoclonal antibodies was ascertained based on their cross-reactivity with porins isolated from S. typhi, Shigella flexneri and Escherichia coli and lipopolysaccharide (LPS) of S. typhimurium and E. coli. Serum samples were found to be positive for porin as early as 3 days after intravenous and 5 days after oral infection. In addition, a positive correlation was observed between the bacterial load and the concentration of porin detected in the sera. On the other hand, analysis of sera for anti-porin antibody showed diametrically opposite time kinetics with antigenaemia. These results indicate that porin accumulates in the serum of infected mice much earlier than the appearance of antibodies to porin. Thus detection of porin holds promise for early diagnosis of typhoid." @default.
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• W2168183931 date "1992-02-01" @default.
• W2168183931 modified "2023-10-18" @default.
• W2168183931 title "Detection of porin antigen in serum for early diagnosis of mouse infections withSalmonella typhimurium" @default.
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• W2168183931 doi "https://doi.org/10.1111/j.1574-6968.1992.tb04981.x" @default.
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