FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2168227311> ?p ?o ?g. }

• W2168227311 endingPage "3595" @default.
• W2168227311 startingPage "3584" @default.
• W2168227311 abstract "HIV-1 Gag assembles into virus particles predominantly at the plasma membrane (PM). Previously, we observed that phosphatidylinositol-(4,5)-bisphosphate [PI(4,5)P(2)] is essential for Gag binding to the plasma membrane and virus release in HeLa cells. In the current study, we found that PI(4,5)P(2) also facilitates Gag binding to the PM and efficient virus release in T cells. Notably, serial passage of HIV-1 in an A3.01 clone that expresses polyphosphoinositide 5-phosphatase IV (5ptaseIV), which depletes cellular PI(4,5)P(2), yielded an adapted mutant with a Leu-to-Arg change at matrix residue 74 (74LR). Virus replication in T cells expressing 5ptaseIV was accelerated by the 74LR mutation relative to replication of wild type HIV-1 (WT). This accelerated replication of the 74LR mutant was not due to improved virus release. In control T cells, the 74LR mutant releases virus less efficiently than does the WT, whereas in cells expressing 5ptaseIV, the WT and the 74LR mutant are similarly inefficient in virus release. Unexpectedly, we found that the 74LR mutation increased virus infectivity and compensated for the inefficient virus release. Altogether, these results indicate that PI(4,5)P(2) is essential for Gag-membrane binding, targeting of Gag to the PM, and efficient virus release in T cells, which in turn likely promotes efficient virus spread in T cell cultures. In T cells with low PI(4,5)P(2) levels, however, the reduced virus particle production can be compensated for by a mutation that enhances virus infectivity." @default.
• W2168227311 created "2016-06-24" @default.
• W2168227311 creator A5003159518 @default.
• W2168227311 creator A5026352857 @default.
• W2168227311 creator A5062424208 @default.
• W2168227311 date "2011-04-01" @default.
• W2168227311 modified "2023-09-24" @default.
• W2168227311 title "Assembly and Replication of HIV-1 in T Cells with Low Levels of Phosphatidylinositol-(4,5)-Bisphosphate" @default.
• W2168227311 cites W1508323061 @default.
• W2168227311 cites W1539979903 @default.
• W2168227311 cites W1634675773 @default.
• W2168227311 cites W170446279 @default.
• W2168227311 cites W1806998329 @default.
• W2168227311 cites W1908410208 @default.
• W2168227311 cites W1962465787 @default.
• W2168227311 cites W1963508527 @default.
• W2168227311 cites W1963814857 @default.
• W2168227311 cites W1964422613 @default.
• W2168227311 cites W1965928113 @default.
• W2168227311 cites W1974756832 @default.
• W2168227311 cites W1978461631 @default.
• W2168227311 cites W1979284427 @default.
• W2168227311 cites W1982997055 @default.
• W2168227311 cites W1986881222 @default.
• W2168227311 cites W1987346882 @default.
• W2168227311 cites W1993145046 @default.
• W2168227311 cites W1999551487 @default.
• W2168227311 cites W1999678180 @default.
• W2168227311 cites W2001430278 @default.
• W2168227311 cites W2006771360 @default.
• W2168227311 cites W2010261424 @default.
• W2168227311 cites W2010313133 @default.
• W2168227311 cites W2010458160 @default.
• W2168227311 cites W2010889827 @default.
• W2168227311 cites W2017522157 @default.
• W2168227311 cites W2017980922 @default.
• W2168227311 cites W2021067210 @default.
• W2168227311 cites W2023916724 @default.
• W2168227311 cites W2028156315 @default.
• W2168227311 cites W2034684407 @default.
• W2168227311 cites W2035694643 @default.
• W2168227311 cites W2037721396 @default.
• W2168227311 cites W2043312574 @default.
• W2168227311 cites W2047452261 @default.
• W2168227311 cites W2050483599 @default.
• W2168227311 cites W2052678796 @default.
• W2168227311 cites W2055921223 @default.
• W2168227311 cites W2057712924 @default.
• W2168227311 cites W2060952336 @default.
• W2168227311 cites W2063613016 @default.
• W2168227311 cites W2072683386 @default.
• W2168227311 cites W2081618928 @default.
• W2168227311 cites W2084383843 @default.
• W2168227311 cites W2085355019 @default.
• W2168227311 cites W2096621401 @default.
• W2168227311 cites W2102229122 @default.
• W2168227311 cites W2102239633 @default.
• W2168227311 cites W2104395143 @default.
• W2168227311 cites W2108623121 @default.
• W2168227311 cites W2115644744 @default.
• W2168227311 cites W2120329437 @default.
• W2168227311 cites W2122077692 @default.
• W2168227311 cites W2122519300 @default.
• W2168227311 cites W2123913956 @default.
• W2168227311 cites W2127153680 @default.
• W2168227311 cites W2136958613 @default.
• W2168227311 cites W2137222057 @default.
• W2168227311 cites W2138263779 @default.
• W2168227311 cites W2139675968 @default.
• W2168227311 cites W2143111754 @default.
• W2168227311 cites W2152307759 @default.
• W2168227311 cites W2154140185 @default.
• W2168227311 cites W2155183647 @default.
• W2168227311 cites W2155233217 @default.
• W2168227311 cites W2157055613 @default.
• W2168227311 cites W2161656950 @default.
• W2168227311 cites W2166678307 @default.
• W2168227311 cites W2168606329 @default.
• W2168227311 cites W2172285292 @default.
• W2168227311 cites W4249774679 @default.
• W2168227311 doi "https://doi.org/10.1128/jvi.02266-10" @default.
• W2168227311 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3067840" @default.
• W2168227311 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21270152" @default.
• W2168227311 hasPublicationYear "2011" @default.
• W2168227311 type Work @default.
• W2168227311 sameAs 2168227311 @default.
• W2168227311 citedByCount "33" @default.
• W2168227311 countsByYear W21682273112012 @default.
• W2168227311 countsByYear W21682273112013 @default.
• W2168227311 countsByYear W21682273112014 @default.
• W2168227311 countsByYear W21682273112015 @default.
• W2168227311 countsByYear W21682273112016 @default.
• W2168227311 countsByYear W21682273112017 @default.
• W2168227311 countsByYear W21682273112019 @default.
• W2168227311 countsByYear W21682273112020 @default.
• W2168227311 countsByYear W21682273112021 @default.
• W2168227311 countsByYear W21682273112022 @default.
• W2168227311 crossrefType "journal-article" @default.

• next