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- W2168381330 abstract "The histone H3 variant CENP-A is normally tightly regulated to ensure only one centromere exists per chromosome. Native CENP-A is often found overexpressed in human cancer cells and a range of human tumors. Consequently, CENP-A misregulation is thought to contribute to genome instability in human cancers. However, the consequences of such overexpression have not been directly elucidated in human cancer cells. To investigate native CENP-A overexpression, we sought to uncover CENP-A-associated defects in human cells. We confirm that CENP-A is innately overexpressed in several colorectal cancer cell lines. In such cells, we report that a subset of structurally distinct CENP-A-containing nucleosomes associate with canonical histone H3, and with the transcription-coupled chaperones ATRX and DAXX. Furthermore, such hybrid CENP-A nucleosomes localize to DNase I hypersensitive and transcription factor binding sites, including at promoters of genes across the human genome. A distinct class of CENP-A hotspots also accumulates at subtelomeric chromosomal locations, including at the 8q24/Myc region long-associated with genomic instability. We show this 8q24 accumulation of CENP-A can also be seen in early stage primary colorectal tumors. Our data demonstrate that excess CENP-A accumulates at noncentromeric locations in the human cancer genome. These findings suggest that ectopic CENP-A nucleosomes could alter the state of the chromatin fiber, potentially impacting gene regulation and chromosome fragility." @default.
- W2168381330 created "2016-06-24" @default.
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- W2168381330 date "2015-01-13" @default.
- W2168381330 modified "2023-10-10" @default.
- W2168381330 title "CENP-A nucleosomes localize to transcription factor hotspots and subtelomeric sites in human cancer cells" @default.
- W2168381330 cites W134483022 @default.
- W2168381330 cites W1532980594 @default.
- W2168381330 cites W1562426635 @default.
- W2168381330 cites W1801126848 @default.
- W2168381330 cites W1957377060 @default.
- W2168381330 cites W1965707365 @default.
- W2168381330 cites W1970549330 @default.
- W2168381330 cites W1970668361 @default.
- W2168381330 cites W1971118090 @default.
- W2168381330 cites W1971651847 @default.
- W2168381330 cites W1972736799 @default.
- W2168381330 cites W1974591830 @default.
- W2168381330 cites W1978631580 @default.
- W2168381330 cites W1979922586 @default.
- W2168381330 cites W1980361305 @default.
- W2168381330 cites W1982519328 @default.
- W2168381330 cites W1992906999 @default.
- W2168381330 cites W1998000745 @default.
- W2168381330 cites W2001269813 @default.
- W2168381330 cites W2002698073 @default.
- W2168381330 cites W2015697863 @default.
- W2168381330 cites W2024133477 @default.
- W2168381330 cites W2027988966 @default.
- W2168381330 cites W2029316600 @default.
- W2168381330 cites W2035458388 @default.
- W2168381330 cites W2038784088 @default.
- W2168381330 cites W2040109903 @default.
- W2168381330 cites W2041808377 @default.
- W2168381330 cites W2041815095 @default.
- W2168381330 cites W2042450235 @default.
- W2168381330 cites W2042491847 @default.
- W2168381330 cites W2045762186 @default.
- W2168381330 cites W2051242796 @default.
- W2168381330 cites W2051390965 @default.
- W2168381330 cites W2055141743 @default.
- W2168381330 cites W2055220220 @default.
- W2168381330 cites W2056107617 @default.
- W2168381330 cites W2059003504 @default.
- W2168381330 cites W2060073273 @default.
- W2168381330 cites W2061540758 @default.
- W2168381330 cites W2063879491 @default.
- W2168381330 cites W2064906213 @default.
- W2168381330 cites W2068599792 @default.
- W2168381330 cites W2068608369 @default.
- W2168381330 cites W2071015129 @default.
- W2168381330 cites W2073493517 @default.
- W2168381330 cites W2073898388 @default.
- W2168381330 cites W2074908472 @default.
- W2168381330 cites W2076170552 @default.
- W2168381330 cites W2081007008 @default.
- W2168381330 cites W2084748434 @default.
- W2168381330 cites W2088118120 @default.
- W2168381330 cites W2088874227 @default.
- W2168381330 cites W2091689158 @default.
- W2168381330 cites W2093207308 @default.
- W2168381330 cites W2094969209 @default.
- W2168381330 cites W2097457499 @default.
- W2168381330 cites W2103089522 @default.
- W2168381330 cites W2104481217 @default.
- W2168381330 cites W2113896971 @default.
- W2168381330 cites W2114175147 @default.
- W2168381330 cites W2117649404 @default.
- W2168381330 cites W2119145839 @default.
- W2168381330 cites W2121989845 @default.
- W2168381330 cites W2124951053 @default.
- W2168381330 cites W2131865749 @default.
- W2168381330 cites W2134989579 @default.
- W2168381330 cites W2136801770 @default.
- W2168381330 cites W2138882380 @default.
- W2168381330 cites W2144792857 @default.
- W2168381330 cites W2146843058 @default.
- W2168381330 cites W2152573465 @default.
- W2168381330 cites W2153056550 @default.
- W2168381330 cites W2158430792 @default.
- W2168381330 cites W2158901355 @default.
- W2168381330 cites W2159079025 @default.
- W2168381330 cites W2166439851 @default.
- W2168381330 cites W2166777583 @default.
- W2168381330 cites W2167869412 @default.
- W2168381330 cites W2171718022 @default.
- W2168381330 cites W2271022063 @default.
- W2168381330 cites W2410128695 @default.
- W2168381330 cites W4230009270 @default.
- W2168381330 cites W4230870835 @default.
- W2168381330 doi "https://doi.org/10.1186/1756-8935-8-2" @default.
- W2168381330 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4363203" @default.