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• W2168389715 endingPage "6696" @default.
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• W2168389715 abstract "ABSTRACT The ligand-gated outer membrane porin FepA serves Escherichia coli as the receptor for the siderophore ferric enterobactin. We characterized the ability of seven analogs of enterobactin to supply iron via FepA by quantitatively measuring the binding and transport of their 59 Fe complexes. The experiments refuted the idea that chirality of the iron complex affects its recognition by FepA and demonstrated the necessity of an unsubstituted catecholate coordination center for binding to the outer membrane protein. Among the compounds we tested, only ferric enantioenterobactin, the synthetic, left-handed isomer of natural enterobactin, and ferric TRENCAM, which substitutes a tertiary amine for the macrocyclic lactone ring of ferric enterobactin but maintains an unsubstituted catecholate iron complex, were recognized by FepA ( K d ≈ 20 nM). Ferric complexes of other analogs (TRENCAM-3,2-HOPO; TREN-Me-3,2-HOPO; MeMEEtTAM; MeME-Me-3,2-HOPO; K 3 MECAMS; agrobactin A) with alterations to the chelating groups and different net charge on the iron center neither adsorbed to nor transported through FepA. We also compared the binding and uptake of ferric enterobactin by homologs of FepA from Bordetella bronchisepticus , Pseudomonas aeruginosa , and Salmonella typhimurium in the native organisms and as plasmid-mediated clones expressed in E. coli . All the transport proteins bound ferric enterobactin with high affinity ( K d ≤ 100 nM) and transported it at comparable rates (≥50 pmol/min/10 9 cells) in their own particular membrane environments. However, the FepA and IroN proteins of S. typhimurium failed to efficiently function in E. coli . For E. coli , S. typhimurium , and P. aeruginosa , the rate of ferric enterobactin uptake was a sigmoidal function of its concentration, indicating a cooperative transport reaction involving multiple interacting binding sites on FepA." @default.
• W2168389715 created "2016-06-24" @default.
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• W2168389715 date "1998-12-15" @default.
• W2168389715 modified "2023-10-17" @default.
• W2168389715 title "Selectivity of Ferric Enterobactin Binding and Cooperativity of Transport in Gram-Negative Bacteria" @default.
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• W2168389715 doi "https://doi.org/10.1128/jb.180.24.6689-6696.1998" @default.
• W2168389715 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/107775" @default.
• W2168389715 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9852016" @default.
• W2168389715 hasPublicationYear "1998" @default.
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