FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2168489363> ?p ?o ?g. }

• W2168489363 endingPage "545" @default.
• W2168489363 startingPage "536" @default.
• W2168489363 abstract "Natural killer (NK) cells differentiated from hematopoietic stem cells (HSCs) may have significant clinical benefits over NK cells from adult donors, including the ability to choose alloreactive donors and potentially more robust in vivo expansion. Stromal-based methods have been used to study the differentiation of NK cells from HSCs. Stroma and cytokines support NK cell differentiation, but may face considerable regulatory hurdles. A recently reported clinical-grade, heparin-based method could serve as an alternative. How the stromal-based and heparin-based approaches compare in terms of NK cell generating efficiency or function is unknown. We show that compared with heparin-based cultures, stroma significantly increases the yield of HSC-derived NK cells by differentiating less-committed progenitors into the NK lineage. NK cells generated by both approaches were similar for most NK-activating and -inhibiting receptors. Although both approaches resulted in a phenotype consistent with CD56bright stage IV NK cells, heparin-based cultures favored the development of CD56+CD16+ cells, whereas stroma produced more NK cell immunoglobulin-like receptor–expressing NK cells, both of which are markers of terminal maturation. At day 21, stromal-based cultures demonstrated significantly more IL-22 production, and both methods yielded similar amounts of IFN-γ production and cytotoxicity by day 35. These findings suggest that heparin-based cultures are an effective replacement for stroma and may facilitate clinical trials testing HSC-derived NK cells. Natural killer (NK) cells differentiated from hematopoietic stem cells (HSCs) may have significant clinical benefits over NK cells from adult donors, including the ability to choose alloreactive donors and potentially more robust in vivo expansion. Stromal-based methods have been used to study the differentiation of NK cells from HSCs. Stroma and cytokines support NK cell differentiation, but may face considerable regulatory hurdles. A recently reported clinical-grade, heparin-based method could serve as an alternative. How the stromal-based and heparin-based approaches compare in terms of NK cell generating efficiency or function is unknown. We show that compared with heparin-based cultures, stroma significantly increases the yield of HSC-derived NK cells by differentiating less-committed progenitors into the NK lineage. NK cells generated by both approaches were similar for most NK-activating and -inhibiting receptors. Although both approaches resulted in a phenotype consistent with CD56bright stage IV NK cells, heparin-based cultures favored the development of CD56+CD16+ cells, whereas stroma produced more NK cell immunoglobulin-like receptor–expressing NK cells, both of which are markers of terminal maturation. At day 21, stromal-based cultures demonstrated significantly more IL-22 production, and both methods yielded similar amounts of IFN-γ production and cytotoxicity by day 35. These findings suggest that heparin-based cultures are an effective replacement for stroma and may facilitate clinical trials testing HSC-derived NK cells." @default.
• W2168489363 created "2016-06-24" @default.
• W2168489363 creator A5007353795 @default.
• W2168489363 creator A5009609420 @default.
• W2168489363 creator A5027151969 @default.
• W2168489363 creator A5037371687 @default.
• W2168489363 creator A5048956159 @default.
• W2168489363 creator A5051041792 @default.
• W2168489363 creator A5054139421 @default.
• W2168489363 creator A5069298631 @default.
• W2168489363 creator A5084167089 @default.
• W2168489363 creator A5085966907 @default.
• W2168489363 date "2012-04-01" @default.
• W2168489363 modified "2023-10-02" @default.
• W2168489363 title "Natural Killer Cell Differentiation from Hematopoietic Stem Cells: A Comparative Analysis of Heparin- and Stromal Cell–Supported Methods" @default.
• W2168489363 cites W1554690897 @default.
• W2168489363 cites W1828610545 @default.
• W2168489363 cites W1930242451 @default.
• W2168489363 cites W1965897738 @default.
• W2168489363 cites W1966426962 @default.
• W2168489363 cites W1967920980 @default.
• W2168489363 cites W1974372561 @default.
• W2168489363 cites W1974562505 @default.
• W2168489363 cites W1980248785 @default.
• W2168489363 cites W1986260722 @default.
• W2168489363 cites W1997651643 @default.
• W2168489363 cites W2000031079 @default.
• W2168489363 cites W2002221648 @default.
• W2168489363 cites W2006295168 @default.
• W2168489363 cites W2006711580 @default.
• W2168489363 cites W2011574962 @default.
• W2168489363 cites W2014437975 @default.
• W2168489363 cites W2017225287 @default.
• W2168489363 cites W2017678578 @default.
• W2168489363 cites W2026884886 @default.
• W2168489363 cites W2030929004 @default.
• W2168489363 cites W2040538634 @default.
• W2168489363 cites W2043437568 @default.
• W2168489363 cites W2044588902 @default.
• W2168489363 cites W2047431874 @default.
• W2168489363 cites W2054693547 @default.
• W2168489363 cites W2055361585 @default.
• W2168489363 cites W2056036165 @default.
• W2168489363 cites W2057781614 @default.
• W2168489363 cites W2065312261 @default.
• W2168489363 cites W2072094250 @default.
• W2168489363 cites W2074431231 @default.
• W2168489363 cites W2075049419 @default.
• W2168489363 cites W2080826082 @default.
• W2168489363 cites W2089947832 @default.
• W2168489363 cites W2108395794 @default.
• W2168489363 cites W2119532085 @default.
• W2168489363 cites W2119695863 @default.
• W2168489363 cites W2139887220 @default.
• W2168489363 cites W2144464061 @default.
• W2168489363 cites W2161629874 @default.
• W2168489363 cites W2162449424 @default.
• W2168489363 cites W2164296311 @default.
• W2168489363 cites W2165635676 @default.
• W2168489363 cites W2165813814 @default.
• W2168489363 cites W2414608702 @default.
• W2168489363 cites W4234951103 @default.
• W2168489363 cites W4242119968 @default.
• W2168489363 cites W4244579502 @default.
• W2168489363 doi "https://doi.org/10.1016/j.bbmt.2011.11.023" @default.
• W2168489363 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3303970" @default.
• W2168489363 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22155502" @default.
• W2168489363 hasPublicationYear "2012" @default.
• W2168489363 type Work @default.
• W2168489363 sameAs 2168489363 @default.
• W2168489363 citedByCount "29" @default.
• W2168489363 countsByYear W21684893632013 @default.
• W2168489363 countsByYear W21684893632014 @default.
• W2168489363 countsByYear W21684893632015 @default.
• W2168489363 countsByYear W21684893632016 @default.
• W2168489363 countsByYear W21684893632017 @default.
• W2168489363 countsByYear W21684893632019 @default.
• W2168489363 countsByYear W21684893632020 @default.
• W2168489363 countsByYear W21684893632021 @default.
• W2168489363 countsByYear W21684893632022 @default.
• W2168489363 countsByYear W21684893632023 @default.
• W2168489363 crossrefType "journal-article" @default.
• W2168489363 hasAuthorship W2168489363A5007353795 @default.
• W2168489363 hasAuthorship W2168489363A5009609420 @default.
• W2168489363 hasAuthorship W2168489363A5027151969 @default.
• W2168489363 hasAuthorship W2168489363A5037371687 @default.
• W2168489363 hasAuthorship W2168489363A5048956159 @default.
• W2168489363 hasAuthorship W2168489363A5051041792 @default.
• W2168489363 hasAuthorship W2168489363A5054139421 @default.
• W2168489363 hasAuthorship W2168489363A5069298631 @default.
• W2168489363 hasAuthorship W2168489363A5084167089 @default.
• W2168489363 hasAuthorship W2168489363A5085966907 @default.
• W2168489363 hasBestOaLocation W21684893631 @default.
• W2168489363 hasConcept C109159458 @default.
• W2168489363 hasConcept C109316439 @default.
• W2168489363 hasConcept C129374314 @default.
• W2168489363 hasConcept C149532602 @default.
• W2168489363 hasConcept C154317977 @default.

• next