FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2168573645> ?p ?o ?g. }

• W2168573645 endingPage "2087" @default.
• W2168573645 startingPage "2075" @default.
• W2168573645 abstract "Abstract Objective Our previous study suggested that growth arrest and DNA damage–inducible protein 45β (GADD45β) prolonged the survival of hypertrophic chondrocytes in the developing mouse embryo. This study was undertaken, therefore, to investigate whether GADD45β plays a role in adult articular cartilage. Methods Gene expression profiles of cartilage from patients with late‐stage osteoarthritis (OA) were compared with those from patients with early OA and normal controls in 2 separate microarray analyses. Histologic features of cartilage were graded using the Mankin scale, and GADD45β was localized by immunohistochemistry. Human chondrocytes were transduced with small interfering RNA (siRNA)–GADD45β or GADD45β‐FLAG. GADD45β and COL2A1 messenger RNA (mRNA) levels were analyzed by real‐time reverse transcriptase–polymerase chain reaction, and promoter activities were analyzed by transient transfection. Cell death was detected by Hoechst 33342 staining of condensed chromatin. Results GADD45β was expressed at higher levels in cartilage from normal donors and patients with early OA than in cartilage from patients with late‐stage OA. All chondrocyte nuclei in normal cartilage immunostained for GADD45β. In early OA cartilage, GADD45β was distributed variably in chondrocyte clusters, in middle and deep zone cells, and in osteophytes. In contrast, COL2A1, other collagen genes, and factors associated with skeletal development were up‐regulated in late OA, compared with early OA or normal cartilage. In overexpression and knockdown experiments, GADD45β down‐regulated COL2A1 mRNA and promoter activity. NF‐κB overexpression increased GADD45β promoter activity, and siRNA‐GADD45β decreased cell survival per se and enhanced tumor necrosis factor α–induced cell death in human articular chondrocytes. Conclusion These observations suggest that GADD45β might play an important role in regulating chondrocyte homeostasis by modulating collagen gene expression and promoting cell survival in normal adult cartilage and in early OA." @default.
• W2168573645 created "2016-06-24" @default.
• W2168573645 creator A5000992292 @default.
• W2168573645 creator A5009342847 @default.
• W2168573645 creator A5018214967 @default.
• W2168573645 creator A5020071691 @default.
• W2168573645 creator A5025221904 @default.
• W2168573645 creator A5028697843 @default.
• W2168573645 creator A5032053408 @default.
• W2168573645 creator A5047465430 @default.
• W2168573645 creator A5054938101 @default.
• W2168573645 creator A5056567815 @default.
• W2168573645 creator A5066271154 @default.
• W2168573645 creator A5068175679 @default.
• W2168573645 creator A5075492712 @default.
• W2168573645 creator A5082987136 @default.
• W2168573645 creator A5088520419 @default.
• W2168573645 date "2008-06-24" @default.
• W2168573645 modified "2023-10-16" @default.
• W2168573645 title "Differential expression of GADD45β in normal and osteoarthritic cartilage: Potential role in homeostasis of articular chondrocytes" @default.
• W2168573645 cites W1534435233 @default.
• W2168573645 cites W1551310212 @default.
• W2168573645 cites W1563090311 @default.
• W2168573645 cites W1608062813 @default.
• W2168573645 cites W1830180739 @default.
• W2168573645 cites W1952701339 @default.
• W2168573645 cites W1965245788 @default.
• W2168573645 cites W1970693983 @default.
• W2168573645 cites W1986442052 @default.
• W2168573645 cites W1990513491 @default.
• W2168573645 cites W1990844960 @default.
• W2168573645 cites W1993949732 @default.
• W2168573645 cites W2000317813 @default.
• W2168573645 cites W2001627351 @default.
• W2168573645 cites W2001790179 @default.
• W2168573645 cites W2003974937 @default.
• W2168573645 cites W2007515325 @default.
• W2168573645 cites W2013064709 @default.
• W2168573645 cites W2015604710 @default.
• W2168573645 cites W2016251547 @default.
• W2168573645 cites W2019233946 @default.
• W2168573645 cites W2031027318 @default.
• W2168573645 cites W2033622832 @default.
• W2168573645 cites W2038437697 @default.
• W2168573645 cites W2043612827 @default.
• W2168573645 cites W2059667269 @default.
• W2168573645 cites W2062522737 @default.
• W2168573645 cites W2064210589 @default.
• W2168573645 cites W2070881814 @default.
• W2168573645 cites W2071497767 @default.
• W2168573645 cites W2072874559 @default.
• W2168573645 cites W2080266387 @default.
• W2168573645 cites W2083555035 @default.
• W2168573645 cites W2084843851 @default.
• W2168573645 cites W2085314940 @default.
• W2168573645 cites W2085871516 @default.
• W2168573645 cites W2088264188 @default.
• W2168573645 cites W2090179549 @default.
• W2168573645 cites W2091235474 @default.
• W2168573645 cites W2093873750 @default.
• W2168573645 cites W2096388167 @default.
• W2168573645 cites W2098115255 @default.
• W2168573645 cites W2098453858 @default.
• W2168573645 cites W2099427592 @default.
• W2168573645 cites W2104500108 @default.
• W2168573645 cites W2109972881 @default.
• W2168573645 cites W2111033589 @default.
• W2168573645 cites W2117058881 @default.
• W2168573645 cites W2117438733 @default.
• W2168573645 cites W2119429559 @default.
• W2168573645 cites W2145684243 @default.
• W2168573645 cites W2167461417 @default.
• W2168573645 cites W2168221175 @default.
• W2168573645 cites W2171409337 @default.
• W2168573645 cites W4292542165 @default.
• W2168573645 doi "https://doi.org/10.1002/art.23504" @default.
• W2168573645 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3950332" @default.
• W2168573645 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18576389" @default.
• W2168573645 hasPublicationYear "2008" @default.
• W2168573645 type Work @default.
• W2168573645 sameAs 2168573645 @default.
• W2168573645 citedByCount "89" @default.
• W2168573645 countsByYear W21685736452012 @default.
• W2168573645 countsByYear W21685736452013 @default.
• W2168573645 countsByYear W21685736452014 @default.
• W2168573645 countsByYear W21685736452015 @default.
• W2168573645 countsByYear W21685736452016 @default.
• W2168573645 countsByYear W21685736452017 @default.
• W2168573645 countsByYear W21685736452018 @default.
• W2168573645 countsByYear W21685736452019 @default.
• W2168573645 countsByYear W21685736452020 @default.
• W2168573645 countsByYear W21685736452021 @default.
• W2168573645 countsByYear W21685736452022 @default.
• W2168573645 countsByYear W21685736452023 @default.
• W2168573645 crossrefType "journal-article" @default.
• W2168573645 hasAuthorship W2168573645A5000992292 @default.
• W2168573645 hasAuthorship W2168573645A5009342847 @default.
• W2168573645 hasAuthorship W2168573645A5018214967 @default.

• next