FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2168610043> ?p ?o ?g. }

• W2168610043 endingPage "2392" @default.
• W2168610043 startingPage "2383" @default.
• W2168610043 abstract "Insulin-like growth factor–binding protein-5 (IGFBP-5) has been shown to bind to fibroblast extracellular matrix (ECM). Extracellular matrix binding of IGFBP-5 leads to a decrease in its affinity for insulin-like growth factor-I (IGF-I), which allows IGF-I to better equilibrate with IGF receptors. When the amount of IGFBP-5 that is bound to ECM is increased by exogenous addition, IGF-I’s effect on fibroblast growth is enhanced. In this study we identified the specific basic residues in IGFBP-5 that mediate its binding to porcine smooth-muscle cell (pSMC) ECM. An IGFBP-5 mutant containing alterations of basic residues at positions 211, 214, 217, and 218 had the greatest reduction in ECM binding, although three other mutants, R214A, R207A/K211N, and K202A/R206N/R207A, also had major decreases. In contrast, three other mutants, R201A/K202N/R206N/R208A, and K217N/R218A and K211N, had only minimal reductions in ECM binding. This suggested that residues R207 and R214 were the most important for binding, whereas alterations in K211 and R218, which align near them, had minimal effects. To determine the effect of a reduction in ECM binding on the cellular replication response to IGF-I, pSMCs were transfected with the mutant cDNAs that encoded the forms of IGFBPs with the greatest changes in ECM binding. The ECM content of IGFBP-5 from cultures expressing the K211N, R214A, R217A/R218A, and K202A/R206N/R207A mutants was reduced by 79.6 and 71.7%, respectively, compared with cells expressing the wild-type protein. In contrast, abundance of the R201A/K202N/R206N/R208A mutant was reduced by only 14%. Cells expressing the two mutants with reduced ECM binding had decreased DNA synthesis responses to IGF-I, but the cells expressing the R201A/K202N/R206N/R208A mutant responded well to IGF-I. The findings suggest that specific basic amino acids at positions 207 and 214 mediate the binding of IGFBP-5 to pSMC/ECM. Smooth-muscle cells that constitutively express the mutants that bind weakly to ECM are less responsive to IGF-I, suggesting that ECM binding of IGFBP-5 is an important variable that determines cellular responsiveness." @default.
• W2168610043 created "2016-06-24" @default.
• W2168610043 creator A5000288481 @default.
• W2168610043 creator A5039368198 @default.
• W2168610043 creator A5042725889 @default.
• W2168610043 creator A5057577889 @default.
• W2168610043 creator A5061686944 @default.
• W2168610043 date "1998-09-01" @default.
• W2168610043 modified "2023-10-16" @default.
• W2168610043 title "Binding of Insulin-like Growth Factor (IGF)–Binding Protein-5 to Smooth-Muscle Cell Extracellular Matrix Is a Major Determinant of the Cellular Response to IGF-I" @default.
• W2168610043 cites W1562216431 @default.
• W2168610043 cites W1605635614 @default.
• W2168610043 cites W1606989141 @default.
• W2168610043 cites W1971563852 @default.
• W2168610043 cites W1972819401 @default.
• W2168610043 cites W1974086084 @default.
• W2168610043 cites W1974873948 @default.
• W2168610043 cites W1975021862 @default.
• W2168610043 cites W1986547865 @default.
• W2168610043 cites W1994560336 @default.
• W2168610043 cites W1998785413 @default.
• W2168610043 cites W1999920704 @default.
• W2168610043 cites W2000394884 @default.
• W2168610043 cites W2003275504 @default.
• W2168610043 cites W2015843482 @default.
• W2168610043 cites W2034967926 @default.
• W2168610043 cites W2039145114 @default.
• W2168610043 cites W2044670939 @default.
• W2168610043 cites W2058870799 @default.
• W2168610043 cites W2059067233 @default.
• W2168610043 cites W2072514327 @default.
• W2168610043 cites W2074747674 @default.
• W2168610043 cites W2083891157 @default.
• W2168610043 cites W2092751827 @default.
• W2168610043 cites W2112692194 @default.
• W2168610043 cites W2133725782 @default.
• W2168610043 cites W2137808717 @default.
• W2168610043 cites W2149724170 @default.
• W2168610043 cites W2158979977 @default.
• W2168610043 cites W2407924808 @default.
• W2168610043 cites W292398390 @default.
• W2168610043 cites W4243642130 @default.
• W2168610043 doi "https://doi.org/10.1091/mbc.9.9.2383" @default.
• W2168610043 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/25505" @default.
• W2168610043 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9725901" @default.
• W2168610043 hasPublicationYear "1998" @default.
• W2168610043 type Work @default.
• W2168610043 sameAs 2168610043 @default.
• W2168610043 citedByCount "73" @default.
• W2168610043 countsByYear W21686100432012 @default.
• W2168610043 countsByYear W21686100432013 @default.
• W2168610043 countsByYear W21686100432015 @default.
• W2168610043 countsByYear W21686100432016 @default.
• W2168610043 countsByYear W21686100432017 @default.
• W2168610043 countsByYear W21686100432018 @default.
• W2168610043 countsByYear W21686100432020 @default.
• W2168610043 crossrefType "journal-article" @default.
• W2168610043 hasAuthorship W2168610043A5000288481 @default.
• W2168610043 hasAuthorship W2168610043A5039368198 @default.
• W2168610043 hasAuthorship W2168610043A5042725889 @default.
• W2168610043 hasAuthorship W2168610043A5057577889 @default.
• W2168610043 hasAuthorship W2168610043A5061686944 @default.
• W2168610043 hasBestOaLocation W21686100432 @default.
• W2168610043 hasConcept C104317684 @default.
• W2168610043 hasConcept C107824862 @default.
• W2168610043 hasConcept C143065580 @default.
• W2168610043 hasConcept C153911025 @default.
• W2168610043 hasConcept C170493617 @default.
• W2168610043 hasConcept C189165786 @default.
• W2168610043 hasConcept C202751555 @default.
• W2168610043 hasConcept C2775960820 @default.
• W2168610043 hasConcept C2780378035 @default.
• W2168610043 hasConcept C2780381497 @default.
• W2168610043 hasConcept C2780689927 @default.
• W2168610043 hasConcept C28406088 @default.
• W2168610043 hasConcept C51639874 @default.
• W2168610043 hasConcept C553089730 @default.
• W2168610043 hasConcept C55493867 @default.
• W2168610043 hasConcept C86803240 @default.
• W2168610043 hasConcept C95444343 @default.
• W2168610043 hasConceptScore W2168610043C104317684 @default.
• W2168610043 hasConceptScore W2168610043C107824862 @default.
• W2168610043 hasConceptScore W2168610043C143065580 @default.
• W2168610043 hasConceptScore W2168610043C153911025 @default.
• W2168610043 hasConceptScore W2168610043C170493617 @default.
• W2168610043 hasConceptScore W2168610043C189165786 @default.
• W2168610043 hasConceptScore W2168610043C202751555 @default.
• W2168610043 hasConceptScore W2168610043C2775960820 @default.
• W2168610043 hasConceptScore W2168610043C2780378035 @default.
• W2168610043 hasConceptScore W2168610043C2780381497 @default.
• W2168610043 hasConceptScore W2168610043C2780689927 @default.
• W2168610043 hasConceptScore W2168610043C28406088 @default.
• W2168610043 hasConceptScore W2168610043C51639874 @default.
• W2168610043 hasConceptScore W2168610043C553089730 @default.
• W2168610043 hasConceptScore W2168610043C55493867 @default.
• W2168610043 hasConceptScore W2168610043C86803240 @default.
• W2168610043 hasConceptScore W2168610043C95444343 @default.
• W2168610043 hasIssue "9" @default.

• next