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• W2168737579 abstract "The ribosomal DNA (rDNA) of Rhagoletis pomonella was localized within both salivary gland polytene chromosomes and somatic cell mitotic chromosomes by in situ hybridization using the heterospecific Drosophila melanogaster rDNA clone, Dm238. In situ hybridization analysis of polytene nuclei showed that R. pomonella rDNA is located in the nucleolus and adjacent granular network of chromosome 1. The site of origin of rDNA is within this isomorphic granular network. The preservation of nucleolar ultrastructure in some polytene chromosome preparations allowed light microscope localization of R. pomonella rDNA to the apparent periphery of fibrillar centres within fibrillar complexes. In somatic cell nuclei, DM238 hybridized to the nucleolus organizing region (NOR) located on chromosome 1 at the site of the secondary constriction. The frequency distribution of heteromorphisms for rDNA content, differential appearance of secondary constrictions, non-pairing of the NOR and differences in homologue lengths suggests that the structural differentiation of this region in chromosome 1 is sex linked. This the first published description of the salivary gland polytene chromosomes from R. pomonella, and we include a tentative karyotype description, polytene chromosome maps and comments on their suitability for banding and molecular analysis." @default.
• W2168737579 created "2016-06-24" @default.
• W2168737579 creator A5046499288 @default.
• W2168737579 creator A5051793179 @default.
• W2168737579 date "1993-11-01" @default.
• W2168737579 modified "2023-10-16" @default.
• W2168737579 title "Localization of ribosomal DNA in Rhagoletis pomonella (Diptera: Tephritidae) by in situ hybridization" @default.
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• W2168737579 doi "https://doi.org/10.1111/j.1365-2583.1993.tb00136.x" @default.
• W2168737579 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9087554" @default.
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