FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2168768749> ?p ?o ?g. }

• W2168768749 abstract "Onychomycosis is a fungal infection of nails, leading to the gradual destruction of the nail plate. Treatment of onychomycosis may need long-time oral antifungal therapy that can have potential side effects, thus accurate diagnosis of the disease before treatment is important. Culture for diagnosis of onychomycosis is time-consuming and has high false-negative rates. To expedite the diagnosis, an oligonucleotide array, based on hybridization between immobilized oligonucleotide probes and PCR products, for direct detection of dermatophytes and Candida albicans in clinical specimens was evaluated. Species-specific oligonucleotide probes designed from the internal transcribed spacer (ITS) regions of the rRNA gene were immobilized on a nylon membrane. The assay procedures consisted of PCR amplification of the ITS using universal primers, followed by hybridization of the digoxigenin-labeled amplicons to probes on the array. Thirty two nail samples (29 patients) were analyzed by the array, and the results were compared with those obtained by culture. Array-positive but culture-negative samples were confirmed by cloning and re-sequencing of the amplified ITS and by reviewing patient’s clinical data. The total recovery of culture and confirmed array-positive but culture-negative results was considered 100% and was used for performance evaluation of both methods. Concordant results were obtained in 21 samples (10 positives and 11 negatives) by both methods. Eleven samples were array-positive but culture-negative; among them, 9 samples were considered true positives after discrepant analysis. Comparing with culture, the array had significantly higher sensitivity [100% (95% CI 82.2% −100%) vs 52.6% (28.9% −75.5%), p <0.001] and negative predictive value [100% (71.3% −100%) vs 59.1% (36.4% −79.3%), p <0.05), while no significant differences were observed in specificity (84.6% vs 100%, p =0.48) and positive predictive value (90.5% vs 100%, p =1.0). The whole procedures of the array were about 24 h, whilst results from culture take 1 to 3 weeks. The array offers an accurate and rapid alternative to culture. Rapid diagnosis can expedite appropriate antifungal treatment of onychomycosis. However, the single site nature of this study conducted at a referral hospital invites caution." @default.
• W2168768749 created "2016-06-24" @default.
• W2168768749 creator A5005041892 @default.
• W2168768749 creator A5017089774 @default.
• W2168768749 creator A5019750615 @default.
• W2168768749 creator A5039220000 @default.
• W2168768749 creator A5042448520 @default.
• W2168768749 creator A5045779083 @default.
• W2168768749 creator A5050433974 @default.
• W2168768749 creator A5076712888 @default.
• W2168768749 date "2014-11-07" @default.
• W2168768749 modified "2023-10-09" @default.
• W2168768749 title "Rapid detection of dermatophytes and Candida albicansin onychomycosis specimens by an oligonucleotide array" @default.
• W2168768749 cites W1519726870 @default.
• W2168768749 cites W1550960553 @default.
• W2168768749 cites W1570153192 @default.
• W2168768749 cites W1771582559 @default.
• W2168768749 cites W1855104919 @default.
• W2168768749 cites W1971131008 @default.
• W2168768749 cites W1974644786 @default.
• W2168768749 cites W1985852982 @default.
• W2168768749 cites W2001375758 @default.
• W2168768749 cites W2022329722 @default.
• W2168768749 cites W2028016768 @default.
• W2168768749 cites W2030193722 @default.
• W2168768749 cites W2040423498 @default.
• W2168768749 cites W2042802905 @default.
• W2168768749 cites W2062423779 @default.
• W2168768749 cites W2066853771 @default.
• W2168768749 cites W2068868525 @default.
• W2168768749 cites W2073299470 @default.
• W2168768749 cites W2076460249 @default.
• W2168768749 cites W2076652311 @default.
• W2168768749 cites W2080484532 @default.
• W2168768749 cites W2081793374 @default.
• W2168768749 cites W2082023874 @default.
• W2168768749 cites W2095361954 @default.
• W2168768749 cites W2098671628 @default.
• W2168768749 cites W2099661956 @default.
• W2168768749 cites W2109108201 @default.
• W2168768749 cites W2109533181 @default.
• W2168768749 cites W2111140247 @default.
• W2168768749 cites W2114171414 @default.
• W2168768749 cites W2119979025 @default.
• W2168768749 cites W2124169039 @default.
• W2168768749 cites W2129822772 @default.
• W2168768749 cites W2141293158 @default.
• W2168768749 cites W2143777984 @default.
• W2168768749 cites W2162914138 @default.
• W2168768749 cites W2170871580 @default.
• W2168768749 cites W4211087045 @default.
• W2168768749 cites W4236886121 @default.
• W2168768749 cites W4254242850 @default.
• W2168768749 cites W4376596050 @default.
• W2168768749 doi "https://doi.org/10.1186/s12879-014-0581-5" @default.
• W2168768749 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4234842" @default.
• W2168768749 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25377491" @default.
• W2168768749 hasPublicationYear "2014" @default.
• W2168768749 type Work @default.
• W2168768749 sameAs 2168768749 @default.
• W2168768749 citedByCount "13" @default.
• W2168768749 countsByYear W21687687492015 @default.
• W2168768749 countsByYear W21687687492016 @default.
• W2168768749 countsByYear W21687687492017 @default.
• W2168768749 countsByYear W21687687492018 @default.
• W2168768749 countsByYear W21687687492020 @default.
• W2168768749 countsByYear W21687687492022 @default.
• W2168768749 countsByYear W21687687492023 @default.
• W2168768749 crossrefType "journal-article" @default.
• W2168768749 hasAuthorship W2168768749A5005041892 @default.
• W2168768749 hasAuthorship W2168768749A5017089774 @default.
• W2168768749 hasAuthorship W2168768749A5019750615 @default.
• W2168768749 hasAuthorship W2168768749A5039220000 @default.
• W2168768749 hasAuthorship W2168768749A5042448520 @default.
• W2168768749 hasAuthorship W2168768749A5045779083 @default.
• W2168768749 hasAuthorship W2168768749A5050433974 @default.
• W2168768749 hasAuthorship W2168768749A5076712888 @default.
• W2168768749 hasBestOaLocation W21687687491 @default.
• W2168768749 hasConcept C104317684 @default.
• W2168768749 hasConcept C129312508 @default.
• W2168768749 hasConcept C155794727 @default.
• W2168768749 hasConcept C190139176 @default.
• W2168768749 hasConcept C49105822 @default.
• W2168768749 hasConcept C54355233 @default.
• W2168768749 hasConcept C67905577 @default.
• W2168768749 hasConcept C8185291 @default.
• W2168768749 hasConcept C86803240 @default.
• W2168768749 hasConcept C89423630 @default.
• W2168768749 hasConceptScore W2168768749C104317684 @default.
• W2168768749 hasConceptScore W2168768749C129312508 @default.
• W2168768749 hasConceptScore W2168768749C155794727 @default.
• W2168768749 hasConceptScore W2168768749C190139176 @default.
• W2168768749 hasConceptScore W2168768749C49105822 @default.
• W2168768749 hasConceptScore W2168768749C54355233 @default.
• W2168768749 hasConceptScore W2168768749C67905577 @default.
• W2168768749 hasConceptScore W2168768749C8185291 @default.
• W2168768749 hasConceptScore W2168768749C86803240 @default.
• W2168768749 hasConceptScore W2168768749C89423630 @default.
• W2168768749 hasIssue "1" @default.
• W2168768749 hasLocation W21687687491 @default.

• next