FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2168824967> ?p ?o ?g. }

• W2168824967 endingPage "14" @default.
• W2168824967 startingPage "1" @default.
• W2168824967 abstract "Recombinant cytokines are valuable tools for functional studies and candidates for vaccine additives or therapeutic use in various diseases. They can also be used to generate specific antibodies to analyze the roles of different cytokines during immune responses. We generated a mammalian expression system for recombinant cytokines using the equine IgG1 heavy chain constant region as a tag for detection and purification of the expressed cytokine, demonstrated here using equine interferon-gamma (IFN-γ), interleukin-2 (IL-2), interleukin-4 (IL4) and transforming growth factor-β1 (TGF-β1). The resulting IgG1 fusion proteins were composed of the C-terminal heavy chain constant region of the IgG1 (IgGa), and the N-terminal cytokine replacing the immunoglobulin heavy chain variable domain. The fusion proteins were expressed in CHO cells as dimers and their structures had similarity to that of IgG heavy chain antibodies. In contrast to other tags, the IgG1 heavy chain constant region allowed the selection for clones secreting high levels of the recombinant protein by a sensitive ELISA. In addition, the IgG1 heavy chain constant region facilitated identification of stable transfectants by flow cytometry and the secreted recombinant fusion protein by SDS-PAGE and Western blotting. To recover the cytokine from the IgG1 fusion partner, an enterokinase cleavage site was cloned between the cytokine gene and the immunoglobulin heavy chain constant region gene. The purification of the fusion protein by protein G affinity columns, the enterokinase digestion of the cytokine from the IgG1 heavy chain region after or during purification, and the biological activity of the cytokine within the fusion protein or after its isolation was demonstrated in detail for equine IFN-γ/IgG1 by up-regulation of major histocompatibility complex (MHC) class II expression on horse lymphocytes. Biological activity could also be confirmed for the IL-2 and IL-4/IgG1 fusion proteins. To test the crossreactivity and specificity of anti-human TGF-β1, and anti-bovine and anti-canine IFN-γ antibodies to respective horse cytokines, the four cytokine/IgG1 fusion proteins were successfully used in ELISA, flow cytometry and/or Western blotting. In summary, equine IgG1 fusion proteins provide a source of recombinant proteins with high structural and functional homology to their native counterparts, including a convenient system for selection of stable, high expressing transfectants, and a means for monitoring specificity of antibodies to equine cytokines." @default.
• W2168824967 created "2016-06-24" @default.
• W2168824967 creator A5011662443 @default.
• W2168824967 creator A5028807186 @default.
• W2168824967 creator A5057290159 @default.
• W2168824967 creator A5074590275 @default.
• W2168824967 creator A5085721840 @default.
• W2168824967 date "2005-05-01" @default.
• W2168824967 modified "2023-10-16" @default.
• W2168824967 title "Horse cytokine/IgG fusion proteins – mammalian expression of biologically active cytokines and a system to verify antibody specificity to equine cytokines" @default.
• W2168824967 cites W1571941406 @default.
• W2168824967 cites W1576091094 @default.
• W2168824967 cites W1968518622 @default.
• W2168824967 cites W1973358897 @default.
• W2168824967 cites W1974290428 @default.
• W2168824967 cites W1976640611 @default.
• W2168824967 cites W1997798081 @default.
• W2168824967 cites W1998814931 @default.
• W2168824967 cites W2019009579 @default.
• W2168824967 cites W2024473186 @default.
• W2168824967 cites W2039270043 @default.
• W2168824967 cites W2056892026 @default.
• W2168824967 cites W2057113600 @default.
• W2168824967 cites W2061263433 @default.
• W2168824967 cites W2062999041 @default.
• W2168824967 cites W2064652766 @default.
• W2168824967 cites W2066203649 @default.
• W2168824967 cites W2067174690 @default.
• W2168824967 cites W2067910785 @default.
• W2168824967 cites W2070953623 @default.
• W2168824967 cites W2073162544 @default.
• W2168824967 cites W2083582256 @default.
• W2168824967 cites W2095170040 @default.
• W2168824967 cites W2108544021 @default.
• W2168824967 cites W2111929893 @default.
• W2168824967 cites W2140200525 @default.
• W2168824967 cites W2170255591 @default.
• W2168824967 cites W2259672406 @default.
• W2168824967 cites W4249619632 @default.
• W2168824967 cites W4313376861 @default.
• W2168824967 doi "https://doi.org/10.1016/j.vetimm.2004.11.010" @default.
• W2168824967 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15797470" @default.
• W2168824967 hasPublicationYear "2005" @default.
• W2168824967 type Work @default.
• W2168824967 sameAs 2168824967 @default.
• W2168824967 citedByCount "48" @default.
• W2168824967 countsByYear W21688249672012 @default.
• W2168824967 countsByYear W21688249672013 @default.
• W2168824967 countsByYear W21688249672014 @default.
• W2168824967 countsByYear W21688249672015 @default.
• W2168824967 countsByYear W21688249672016 @default.
• W2168824967 countsByYear W21688249672017 @default.
• W2168824967 countsByYear W21688249672018 @default.
• W2168824967 countsByYear W21688249672019 @default.
• W2168824967 countsByYear W21688249672021 @default.
• W2168824967 countsByYear W21688249672022 @default.
• W2168824967 crossrefType "journal-article" @default.
• W2168824967 hasAuthorship W2168824967A5011662443 @default.
• W2168824967 hasAuthorship W2168824967A5028807186 @default.
• W2168824967 hasAuthorship W2168824967A5057290159 @default.
• W2168824967 hasAuthorship W2168824967A5074590275 @default.
• W2168824967 hasAuthorship W2168824967A5085721840 @default.
• W2168824967 hasConcept C104317684 @default.
• W2168824967 hasConcept C123894998 @default.
• W2168824967 hasConcept C153911025 @default.
• W2168824967 hasConcept C159654299 @default.
• W2168824967 hasConcept C203014093 @default.
• W2168824967 hasConcept C2778690821 @default.
• W2168824967 hasConcept C36394416 @default.
• W2168824967 hasConcept C40767141 @default.
• W2168824967 hasConcept C55493867 @default.
• W2168824967 hasConcept C86803240 @default.
• W2168824967 hasConcept C96007430 @default.
• W2168824967 hasConceptScore W2168824967C104317684 @default.
• W2168824967 hasConceptScore W2168824967C123894998 @default.
• W2168824967 hasConceptScore W2168824967C153911025 @default.
• W2168824967 hasConceptScore W2168824967C159654299 @default.
• W2168824967 hasConceptScore W2168824967C203014093 @default.
• W2168824967 hasConceptScore W2168824967C2778690821 @default.
• W2168824967 hasConceptScore W2168824967C36394416 @default.
• W2168824967 hasConceptScore W2168824967C40767141 @default.
• W2168824967 hasConceptScore W2168824967C55493867 @default.
• W2168824967 hasConceptScore W2168824967C86803240 @default.
• W2168824967 hasConceptScore W2168824967C96007430 @default.
• W2168824967 hasIssue "1-2" @default.
• W2168824967 hasLocation W21688249671 @default.
• W2168824967 hasLocation W21688249672 @default.
• W2168824967 hasOpenAccess W2168824967 @default.
• W2168824967 hasPrimaryLocation W21688249671 @default.
• W2168824967 hasRelatedWork W2348169472 @default.
• W2168824967 hasRelatedWork W2348991363 @default.
• W2168824967 hasRelatedWork W2349480482 @default.
• W2168824967 hasRelatedWork W2349682198 @default.
• W2168824967 hasRelatedWork W2356557998 @default.
• W2168824967 hasRelatedWork W2357972350 @default.
• W2168824967 hasRelatedWork W2365212916 @default.
• W2168824967 hasRelatedWork W2381845779 @default.
• W2168824967 hasRelatedWork W2389269354 @default.

• next