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• W2168857380 abstract "ABSTRACT The binding of bacteria and platelets may play a central role in the pathogenesis of infective endocarditis. Platelet binding by Streptococcus gordonii strain M99 is predominantly mediated by the 286-kDa cell wall-anchored protein GspB. This unusually large protein lacks a typical amino-terminal signal peptide and is translocated from the cytoplasm via a dedicated transport system. A 14-kb segment just downstream of gspB encodes SecA2 and SecY2, two components of the GspB-specific transport system. The downstream segment also encodes several putative glycosyl transferases that may be responsible for the posttranslational modification of GspB. In this study, we compared the abilities of M99 and two GspB − mutant strains to bind various lectins. GspB was found to have affinity for lectins that bind N -acetylglucosamine. We also examined variant forms of GspB that lack a carboxy-terminal cell wall-anchoring domain and thus are free of covalent linkage to cell wall peptidoglycan. Like native GspB, these truncated proteins appear to be heavily glycosylated, as evidenced by migration during sodium dodecyl sulfate-polyacrylamide gel electrophoresis with an apparent molecular mass >100 kDa in excess of the predicted mass, negligible staining with conventional protein stains, and reactivity with hydrazide following periodate oxidation. Furthermore, analysis of the carbohydrate associated with the GspB variants by high-pH anion-exchange chromatography revealed the presence of ∼70 to 100 monosaccharide residues per GspB polypeptide (primarily N -acetylglucosamine and glucose). Analysis of GspB in protoplasts of s ecA2 or secY2 mutant strains, which do not export GspB, indicates that GspB is glycosylated in the cytoplasm of these strains. The combined data suggest that the native GspB is a glycoprotein and that it may be glycosylated prior to export." @default.
• W2168857380 created "2016-06-24" @default.
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• W2168857380 date "2004-02-01" @default.
• W2168857380 modified "2023-10-18" @default.
• W2168857380 title "The <i>Streptococcus gordonii</i> Platelet BindingProtein GspB Undergoes Glycosylation Independently ofExport" @default.
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• W2168857380 doi "https://doi.org/10.1128/jb.186.3.638-645.2004" @default.
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