FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2168962275> ?p ?o ?g. }

• W2168962275 endingPage "e3117" @default.
• W2168962275 startingPage "e3117" @default.
• W2168962275 abstract "Trypanosoma cruzi, the aetiological agent of Chagas disease possess extensive genetic diversity. This has led to the development of a plethora of molecular typing methods for the identification of both the known major genetic lineages and for more fine scale characterization of different multilocus genotypes within these major lineages. Whole genome sequencing applied to large sample sizes is not currently viable and multilocus enzyme electrophoresis, the previous gold standard for T. cruzi typing, is laborious and time consuming. In the present work, we present an optimized Multilocus Sequence Typing (MLST) scheme, based on the combined analysis of two recently proposed MLST approaches. Here, thirteen concatenated gene fragments were applied to a panel of T. cruzi reference strains encompassing all known genetic lineages. Concatenation of 13 fragments allowed assignment of all strains to the predicted Discrete Typing Units (DTUs), or near-clades, with the exception of one strain that was an outlier for TcV, due to apparent loss of heterozygosity in one fragment. Monophyly for all DTUs, along with robust bootstrap support, was restored when this fragment was subsequently excluded from the analysis. All possible combinations of loci were assessed against predefined criteria with the objective of selecting the most appropriate combination of between two and twelve fragments, for an optimized MLST scheme. The optimum combination consisted of 7 loci and discriminated between all reference strains in the panel, with the majority supported by robust bootstrap values. Additionally, a reduced panel of just 4 gene fragments displayed high bootstrap values for DTU assignment and discriminated 21 out of 25 genotypes. We propose that the seven-fragment MLST scheme could be used as a gold standard for T. cruzi typing, against which other typing approaches, particularly single locus approaches or systematic PCR assays based on amplicon size, could be compared." @default.
• W2168962275 created "2016-06-24" @default.
• W2168962275 creator A5007357728 @default.
• W2168962275 creator A5007777158 @default.
• W2168962275 creator A5008608631 @default.
• W2168962275 creator A5018809366 @default.
• W2168962275 creator A5025854394 @default.
• W2168962275 creator A5043126491 @default.
• W2168962275 creator A5044195708 @default.
• W2168962275 creator A5052988670 @default.
• W2168962275 creator A5057832799 @default.
• W2168962275 creator A5057997250 @default.
• W2168962275 creator A5060778694 @default.
• W2168962275 creator A5070840083 @default.
• W2168962275 creator A5086690561 @default.
• W2168962275 creator A5089648565 @default.
• W2168962275 date "2014-08-28" @default.
• W2168962275 modified "2023-10-06" @default.
• W2168962275 title "Optimized Multilocus Sequence Typing (MLST) Scheme for Trypanosoma cruzi" @default.
• W2168962275 cites W1567221943 @default.
• W2168962275 cites W1965981590 @default.
• W2168962275 cites W1968052557 @default.
• W2168962275 cites W1975261050 @default.
• W2168962275 cites W1979692840 @default.
• W2168962275 cites W1987784191 @default.
• W2168962275 cites W1988325090 @default.
• W2168962275 cites W1994995217 @default.
• W2168962275 cites W1996489389 @default.
• W2168962275 cites W1996687891 @default.
• W2168962275 cites W1998110890 @default.
• W2168962275 cites W1998576967 @default.
• W2168962275 cites W2000549799 @default.
• W2168962275 cites W2008908497 @default.
• W2168962275 cites W2009151113 @default.
• W2168962275 cites W2011873464 @default.
• W2168962275 cites W2021267302 @default.
• W2168962275 cites W2037329917 @default.
• W2168962275 cites W2044388526 @default.
• W2168962275 cites W2045346667 @default.
• W2168962275 cites W2049290286 @default.
• W2168962275 cites W2051329605 @default.
• W2168962275 cites W2052760269 @default.
• W2168962275 cites W2053485796 @default.
• W2168962275 cites W2059134652 @default.
• W2168962275 cites W2061246349 @default.
• W2168962275 cites W2074044001 @default.
• W2168962275 cites W2080926640 @default.
• W2168962275 cites W2085434874 @default.
• W2168962275 cites W2086589284 @default.
• W2168962275 cites W2089290086 @default.
• W2168962275 cites W2089968521 @default.
• W2168962275 cites W2090948051 @default.
• W2168962275 cites W2095101507 @default.
• W2168962275 cites W2096208713 @default.
• W2168962275 cites W2108643808 @default.
• W2168962275 cites W2109695781 @default.
• W2168962275 cites W2112479590 @default.
• W2168962275 cites W2113019075 @default.
• W2168962275 cites W2121757615 @default.
• W2168962275 cites W2125302790 @default.
• W2168962275 cites W2127223602 @default.
• W2168962275 cites W2129392035 @default.
• W2168962275 cites W2130234251 @default.
• W2168962275 cites W2132149924 @default.
• W2168962275 cites W2147830249 @default.
• W2168962275 cites W2151055938 @default.
• W2168962275 cites W2152450834 @default.
• W2168962275 cites W2153164685 @default.
• W2168962275 cites W2155147988 @default.
• W2168962275 cites W2156470173 @default.
• W2168962275 cites W2162122300 @default.
• W2168962275 cites W2163005628 @default.
• W2168962275 cites W2165471800 @default.
• W2168962275 cites W2167385634 @default.
• W2168962275 cites W2169307994 @default.
• W2168962275 cites W3133382011 @default.
• W2168962275 doi "https://doi.org/10.1371/journal.pntd.0003117" @default.
• W2168962275 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4148231" @default.
• W2168962275 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25167160" @default.
• W2168962275 hasPublicationYear "2014" @default.
• W2168962275 type Work @default.
• W2168962275 sameAs 2168962275 @default.
• W2168962275 citedByCount "30" @default.
• W2168962275 countsByYear W21689622752014 @default.
• W2168962275 countsByYear W21689622752015 @default.
• W2168962275 countsByYear W21689622752016 @default.
• W2168962275 countsByYear W21689622752017 @default.
• W2168962275 countsByYear W21689622752018 @default.
• W2168962275 countsByYear W21689622752019 @default.
• W2168962275 countsByYear W21689622752020 @default.
• W2168962275 countsByYear W21689622752021 @default.
• W2168962275 countsByYear W21689622752023 @default.
• W2168962275 crossrefType "journal-article" @default.
• W2168962275 hasAuthorship W2168962275A5007357728 @default.
• W2168962275 hasAuthorship W2168962275A5007777158 @default.
• W2168962275 hasAuthorship W2168962275A5008608631 @default.
• W2168962275 hasAuthorship W2168962275A5018809366 @default.
• W2168962275 hasAuthorship W2168962275A5025854394 @default.

• next