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• W2168996967 abstract "RNA is involved in a variety of chromatin modification events, ranging from large-scale structural rearrangements to subtle local affects. Here, we extend the evidence for RNA–chromatin interactions to the centromere core. The data indicate that maize centromeric retrotransposons (CRMs) and satellite repeats (CentC) are not only transcribed, but that nearly half of the CRM and CentC RNA is tightly bound to centromeric histone H3 (CENH3), a key inner kinetochore protein. RNAs from another tandem repeat (180-bp knob sequence) or an abundant euchromatic retroelement (Opie) are undetectable within the same anti-CENH3 immune complexes. Both sense and antisense strands of CRM and CentC, but not small interfering RNAs homologous to either repeat, were found to coimmunoprecipitate with CENH3. The bulk of the immunoprecipitated RNA ranged in size from 40 to 200 nt. These data provide evidence for a pool of protected, single-stranded centromeric RNA within the centromere/kinetochore complex." @default.
• W2168996967 created "2016-06-24" @default.
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• W2168996967 date "2004-10-28" @default.
• W2168996967 modified "2023-10-01" @default.
• W2168996967 title "Centromere-encoded RNAs are integral components of the maize kinetochore" @default.
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• W2168996967 doi "https://doi.org/10.1073/pnas.0407154101" @default.
• W2168996967 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/528775" @default.
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