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- W2168998506 abstract "Abstract The metacercaria of the heterophyid digenean Cryptocotyle concavum (Creplin, 1825) Lühe 1899, as seen by light and transmission electron microscopy, from the common goby ( Pomatoschistus microps ) is enclosed within a rigid cyst wall composed of four layers of parasite origin surrounded by a host-derived capsule. The encysted parasite contains anterior glands with secretion-filled ducts opening at the anterior extremity of the ‘head’. Excystment in vitro occurred most readily at 40–42°C on treatment with alkaline bile salts following pretreatment with acid pepsin. After 1 min in pepsin, the metacercaria became intermittently active, periods of rotation lasting 1–3 sec alternating with quiescent periods of 20–40 sec. Metacercariae excysted, some in less than 1 min, after transfer to bile salts. The metacercaria emerged through a hole, which appeared in the cyst wall at the site of application of the ‘head’ of the parasite. Some of the anterior gland ducts of excysted metacercariae were empty, indicating that their secretions are involved in penetrating the cyst wall. Following pretreatment in acid pepsin, no excystment occurred in EBSS (Earle's balanced salt solution) ranging in pH from 2.0 to 4.0, but above pH 4.0, excystment increased, reaching a maximum between pH 7.5 and 8.5. Pepsin and bile salts were not essential for excystment and metacercariae emerged, although less readily, in alkaline EBSS with or without acid EBSS pretreatment. Some emerged in distilled water with no pretreatment. Although no excystment occurred in acid pepsin or in EBSS at pH 2.0 and already-excysted metacercariae were rapidly killed by acid (pH 2.0), acid pepsin pretreatment enhanced excystment in alkaline bile salts." @default.
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- W2168998506 title "In vitro excystment of the metacercaria of Cryptocotyle concavum from the common goby Pomatoschistus microps" @default.
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