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• W2169010275 abstract "To investigate the effect of human oviductal cell coculture on the incidence of apoptosis in mouse embryos.Experimental laboratory study.University gynecology unit.Fallopian tubes were obtained from patients undergoing hysterectomy.Mouse embryos were cocultured with human oviductal cells.Blastocyst development, allocation of inner cell mass (ICM) and trophectoderm (TE) in blastocyst, and apoptosis in embryos.Oviductal cells significantly enhanced the blastulation (38%) and hatching rate (22%) of the cocultured zygotes. The corresponding values in medium alone culture were 21% and 9%, respectively. The cocultured embryos also had higher blastomere count at blastocyst stage (P<0. 005). This was due to increase in both the cell count of ICM (P<0. 05) and TE (P<0.001). Coculture reduced the incidence of apoptosis in the cultured morula and blastocyst from 38% and 48% to 16% (P<0. 001) and 27% (P<0.05), respectively. The number of apoptotic blastomeres per morula (1.5 +/- 0.6; P<0.005) and blastocyst (2.3 +/- 0.7; P<0.005) after coculture was also significantly lower than that of the corresponding control (morula, 2.1 +/- 0.8; blastocyst, 3.5 +/- 1.1).Human oviductal cells improved mouse embryo development partly by decreasing the incidence of apoptosis." @default.
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• W2169010275 date "2000-12-01" @default.
• W2169010275 modified "2023-10-09" @default.
• W2169010275 title "Human oviductal cells reduce the incidence of apoptosis in cocultured mouse embryos" @default.
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• W2169010275 doi "https://doi.org/10.1016/s0015-0282(00)01618-6" @default.
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