FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2169013548> ?p ?o ?g. }

• W2169013548 endingPage "817" @default.
• W2169013548 startingPage "811" @default.
• W2169013548 abstract "Mammalian target of rapamycin (mTOR), a large multidomain protein kinase, regulates cell growth and metabolism in response to environmental signals. The FKBP rapamycin-binding (FRB) domain of mTOR is a validated therapeutic target for the development of immunosuppressant and anticancer drugs but is labile and insoluble. Here we designed a fusion protein between FKBP12 and the FRB domain of mTOR. The fusion protein was successfully expressed in Escherichia coli as a soluble form, and was purified by a simple two-step chromatographic procedure. The fusion protein exhibited increased solubility and stability compared with the isolated FRB domain, and facilitated the analysis of rapamycin and FK506 binding using differential scanning calorimetry (DSC) and solution nuclear magnetic resonance (NMR). DSC enabled the rapid observation of protein-drug interactions at the domain level, while NMR gave insights into the protein-drug interactions at the residue level. The use of the FKBP12-FRB fusion protein combined with DSC and NMR provides a useful tool for the efficient screening of FKBP12-dependent as well as -independent inhibitors of the mTOR FRB domain." @default.
• W2169013548 created "2016-06-24" @default.
• W2169013548 creator A5010269762 @default.
• W2169013548 creator A5011739627 @default.
• W2169013548 creator A5020789321 @default.
• W2169013548 creator A5024419745 @default.
• W2169013548 creator A5025281699 @default.
• W2169013548 creator A5027350918 @default.
• W2169013548 creator A5030361679 @default.
• W2169013548 creator A5051048286 @default.
• W2169013548 creator A5073307399 @default.
• W2169013548 date "2011-09-06" @default.
• W2169013548 modified "2023-10-16" @default.
• W2169013548 title "An evaluation tool for FKBP12-dependent and -independent mTOR inhibitors using a combination of FKBP-mTOR fusion protein, DSC and NMR" @default.
• W2169013548 cites W1546565225 @default.
• W2169013548 cites W1970002782 @default.
• W2169013548 cites W1974555973 @default.
• W2169013548 cites W1975107946 @default.
• W2169013548 cites W1984754431 @default.
• W2169013548 cites W1985046929 @default.
• W2169013548 cites W2010488656 @default.
• W2169013548 cites W2013453542 @default.
• W2169013548 cites W2018046436 @default.
• W2169013548 cites W2024511394 @default.
• W2169013548 cites W2028879260 @default.
• W2169013548 cites W2035965301 @default.
• W2169013548 cites W2036217489 @default.
• W2169013548 cites W2040556540 @default.
• W2169013548 cites W2048525679 @default.
• W2169013548 cites W2063013559 @default.
• W2169013548 cites W2065887259 @default.
• W2169013548 cites W2083982117 @default.
• W2169013548 cites W2090308278 @default.
• W2169013548 cites W2119355002 @default.
• W2169013548 cites W2120918747 @default.
• W2169013548 cites W2134138119 @default.
• W2169013548 cites W2149707682 @default.
• W2169013548 cites W2150028503 @default.
• W2169013548 cites W2152289580 @default.
• W2169013548 cites W2329573625 @default.
• W2169013548 cites W2517054293 @default.
• W2169013548 cites W2782175582 @default.
• W2169013548 cites W4238567307 @default.
• W2169013548 doi "https://doi.org/10.1093/protein/gzr045" @default.
• W2169013548 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3196870" @default.
• W2169013548 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21900305" @default.
• W2169013548 hasPublicationYear "2011" @default.
• W2169013548 type Work @default.
• W2169013548 sameAs 2169013548 @default.
• W2169013548 citedByCount "7" @default.
• W2169013548 countsByYear W21690135482013 @default.
• W2169013548 countsByYear W21690135482014 @default.
• W2169013548 countsByYear W21690135482017 @default.
• W2169013548 countsByYear W21690135482018 @default.
• W2169013548 crossrefType "journal-article" @default.
• W2169013548 hasAuthorship W2169013548A5010269762 @default.
• W2169013548 hasAuthorship W2169013548A5011739627 @default.
• W2169013548 hasAuthorship W2169013548A5020789321 @default.
• W2169013548 hasAuthorship W2169013548A5024419745 @default.
• W2169013548 hasAuthorship W2169013548A5025281699 @default.
• W2169013548 hasAuthorship W2169013548A5027350918 @default.
• W2169013548 hasAuthorship W2169013548A5030361679 @default.
• W2169013548 hasAuthorship W2169013548A5051048286 @default.
• W2169013548 hasAuthorship W2169013548A5073307399 @default.
• W2169013548 hasBestOaLocation W21690135482 @default.
• W2169013548 hasConcept C104317684 @default.
• W2169013548 hasConcept C123894998 @default.
• W2169013548 hasConcept C12554922 @default.
• W2169013548 hasConcept C133956753 @default.
• W2169013548 hasConcept C138885662 @default.
• W2169013548 hasConcept C143065580 @default.
• W2169013548 hasConcept C158525013 @default.
• W2169013548 hasConcept C183713625 @default.
• W2169013548 hasConcept C185592680 @default.
• W2169013548 hasConcept C32619005 @default.
• W2169013548 hasConcept C40767141 @default.
• W2169013548 hasConcept C41895202 @default.
• W2169013548 hasConcept C55493867 @default.
• W2169013548 hasConcept C62478195 @default.
• W2169013548 hasConcept C86554907 @default.
• W2169013548 hasConcept C86803240 @default.
• W2169013548 hasConcept C95444343 @default.
• W2169013548 hasConceptScore W2169013548C104317684 @default.
• W2169013548 hasConceptScore W2169013548C123894998 @default.
• W2169013548 hasConceptScore W2169013548C12554922 @default.
• W2169013548 hasConceptScore W2169013548C133956753 @default.
• W2169013548 hasConceptScore W2169013548C138885662 @default.
• W2169013548 hasConceptScore W2169013548C143065580 @default.
• W2169013548 hasConceptScore W2169013548C158525013 @default.
• W2169013548 hasConceptScore W2169013548C183713625 @default.
• W2169013548 hasConceptScore W2169013548C185592680 @default.
• W2169013548 hasConceptScore W2169013548C32619005 @default.
• W2169013548 hasConceptScore W2169013548C40767141 @default.
• W2169013548 hasConceptScore W2169013548C41895202 @default.
• W2169013548 hasConceptScore W2169013548C55493867 @default.
• W2169013548 hasConceptScore W2169013548C62478195 @default.
• W2169013548 hasConceptScore W2169013548C86554907 @default.
• W2169013548 hasConceptScore W2169013548C86803240 @default.

• next