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• W2169042900 abstract "Reduced coenzyme F420 (F420H2) is an essential intermediate in methanogenesis from CO2. During methanogenesis from H2 and CO2, F420H2 is provided by the action of F420-reducing hydrogenases. However, an alternative pathway has been proposed, where H2-dependent methylenetetrahydromethanopterin dehydrogenase (Hmd) and F420H2-dependent methylenetetrahydromethanopterin dehydrogenase (Mtd) together reduce F420 with H2. Here we report the construction of mutants of Methanococcus maripaludis that are defective in each putative pathway. Their analysis demonstrates that either pathway supports growth on H2 and CO2. Furthermore, we show that during growth on formate instead of H2, where F420H2 is a direct product of formate oxidation, H2 production occurs. H2 presumably arises from the oxidation of F420H2, and the analysis of the mutants during growth on formate suggests that this too can occur by either pathway. We designate the alternative pathway for the interconversion of H2 and F420H2 the Hmd-Mtd cycle." @default.
• W2169042900 created "2016-06-24" @default.
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• W2169042900 date "2008-07-15" @default.
• W2169042900 modified "2023-10-17" @default.
• W2169042900 title "Roles of Coenzyme F ₄₂₀ -Reducing Hydrogenases and Hydrogen- and F ₄₂₀ -Dependent Methylenetetrahydromethanopterin Dehydrogenases in Reduction of F ₄₂₀ and Production of Hydrogen during Methanogenesis" @default.
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• W2169042900 doi "https://doi.org/10.1128/jb.00255-08" @default.
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